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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ITGA5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ITGA5 N/A
Sequence Length (AA) Molecular Weight (Da)
1053 114417
Protein Name
Uncharacterized protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRGAGLPEGA AGARGPRGRP APPLPLLPLL LLLLLPPPPR AAGFNLDAQA
60 70 80 90 100
PAVLSGPPRS FFGFAVDFYR PGARGVSVLV GAPKANTSQP GVLQGGAVYL
110 120 130 140 150
CPWGAGRGHC TPIEFDSKGS RVLESSVSSP DGEEPVEYKS LQWFGATVRA
160 170 180 190 200
HGSSILACAP LYSWRTEKEP LSDPVGTCYL STDNFTRILE YAPCRSDFSW
210 220 230 240 250
AAGQGYCQGG FSAEFTKTGR VVLGGPGSYF WQGQILSATQ EQIAESYYPE
260 270 280 290 300
YLINLVQGQL QTRQASSIYD DSYLGYSVAV GEFSGDDTED FVAGVPKGNL
310 320 330 340 350
TYGYVTILNG SDIRSLYNFS GEQMASYFGY AVAATDTNGD GLDDLLVGAP
360 370 380 390 400
LLMERTADGR PQEVGRVYIY LQHPAGMEPT PTLTLTGQDE FGRFGSSLTP
410 420 430 440 450
LGDLDQDGYN DIAIGAPFGG ETQQGVVLIF PGGPGGPGSQ PSQVLLPLWA
460 470 480 490 500
AGHTPHFFGS ALRGGRDLDG NGYPDLIVGS FGVDKAVVYR GRPIVSTSAS
510 520 530 540 550
LTIFPAMFNP EERSCSLEGN PVACINLSFC LNASGKHVPD SIGFTVELQL
560 570 580 590 600
DWQKQKGGVR RALFLASRQA TLTQTLLIQN GAREDCREMK VYLRNESEFR
610 620 630 640 650
DKLSPIHIAL NFSLDPQAPV DAHGLRPVLH YQSKSRIEDK AQILLDCGED
660 670 680 690 700
NICVPDLQLE VFGDQNHVYL GDKNSLNLTF HAQNVGEGGA YEAELRVTAP
710 720 730 740 750
PEAEYSGLVR NPGNFSSLSC DYFAVNQSRL LVCDLGNPMK AGASIWGGLR
760 770 780 790 800
FTVPHLRDTK KTIQFDFQIL SKNLNNSQSE VVSFPLSVEA SAQVSLNGVS
810 820 830 840 850
KPEAVLFPAS DWRPREQPQE EGDVGPAVQH VYELINQGPS SISRGVLELS
860 870 880 890 900
CPRTLEGQQL LYVTRVEGLS NCTSSHPPNP ESLELDPEGP QHHRLQRREA
910 920 930 940 950
PGRGPGSSGP QILKCPEAEC FRLRCELGPL HRQESRSLQL HFRVWAKTFL
960 970 980 990 1000
QREHQPFSLQ CEAVYEALRM PYRILPQQLP RRELQVATAV QWTKAEGSHG
1010 1020 1030 1040 1050
VPLWIIILAI LVGLLLLGLL IYILYKLGFF KRSLPYGTAM EKAQLKPPAT
1053
SDA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3271 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGA5
Peptide Sequence
GNLTYGYVTILNGSDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
298
Peptide End
314
CPTAC ID
CPTAC-3271
Peptide Molecular Mass
1,854.9476
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 8.9 6.4 4.6 13.3 9.1 23.7 16 11.1 24.1 15 15 15
y7 (1+) 21.9 8.9 4.4 25.5 12.8 25.3 33.6 15.6 25.7 15 15 15
y9 (1+) 9.4 4.6 3.6 16 10.8 23 18.6 11.7 23.3 15 15 15
sum 8.7 3.8 3.4 15.8 10 23.7 18 10.7 23.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Assay Details for CPTAC-3272 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGA5
Peptide Modified Sequence
GN[+1.0]LTYGYVTILNGSDIR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
299
Peptide - Site of Modification
2
Peptide Start
298
Peptide End
314
CPTAC ID
CPTAC-3272
Peptide Molecular Mass
1,855.9316
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.4 6.2 3.4 12.9 10.9 21 17.9 12.5 21.3 15 15 15
y7 (1+) 13.7 5.2 5.7 15.7 11.3 20.5 20.8 12.4 21.3 15 15 15
y10 (1+) 14.3 4.6 5.8 23.8 12.5 19.8 27.8 13.3 20.6 15 15 15
sum 6.6 4.8 3.8 14.8 11.1 20.3 16.2 12.1 20.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3276 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGA5
Peptide Sequence
GNLTYGYVTILNGSDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
298
Peptide End
314
CPTAC ID
CPTAC-3276
Peptide Molecular Mass
1,854.9476
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.3 6 8.6 15.9 11.1 27.5 20.7 12.6 28.8 15 15 15
y9 (1+) 8.9 5.3 10.6 11.8 12.8 27.7 14.8 13.9 29.7 15 15 15
y7 (1+) 13.2 6.2 9.8 13.5 11.1 25.8 18.9 12.7 27.6 15 15 15
sum 7.3 4.1 9.1 10.8 11.3 26.7 13 12 28.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3277 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGA5
Peptide Modified Sequence
GN[+1.0]LTYGYVTILNGSDIR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
299
Peptide - Site of Modification
2
Peptide Start
298
Peptide End
314
CPTAC ID
CPTAC-3277
Peptide Molecular Mass
1,855.9316
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7.8 7.2 7.9 14.1 19.7 32.1 16.1 21 33.1 15 15 15
y9 (1+) 4.3 6.7 6.4 14 19.9 32 14.6 21 32.6 15 15 15
y7 (1+) 8.3 7.3 8.8 16 18.7 32.3 18 20.1 33.5 15 15 15
sum 3.8 6.3 7.1 13.7 19.3 32.1 14.2 20.3 32.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments