BGN, CPTAC-3278 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BGN	N/A

Sequence Length (AA)	Molecular Weight (Da)
369	41566

Protein Name
Biglycan

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWPLWLVASL	LALSQALPFE	QKGFWDFTLD	DGLPMLNDEE	ASGAETTSGV
60	70	80	90	100
PDLDALTPTY	SAMCPFGCHC	HLRVVQCSDL	GLKAVPKEIS	PDTMLLDLQN
110	120	130	140	150
NDISELRADD	FKGLHHLYAL	VLVNNKISKI	HEKAFSPLRK	LQKLYISKNH
160	170	180	190	200
LVEIPPNLPS	SLVELRIHDN	RIRKVPKGVF	SGLRNMNCIE	MGGNPLENSG
210	220	230	240	250
FEPGAFDGLK	LNYLRISEAK	LTGIPKDLPE	TLNELHLDHN	KIQAIELEDL
260	270	280	290	300
LRYSKLYRLG	LGHNQIRMIE	NGSLSFLPTL	RELHLDNNKL	SRVPSGLPDL
310	320	330	340	350
KLLQVVYLHT	NNITKVGVND	FCPVGFGVKR	AYYNGISLFN	NPVPYWEVQP
360	369
ATFRCVTDRL	AIQFGNYKK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3273 Collapse assay details

Data source: Panorama

Official Gene Symbol: BGN
Peptide Modified Sequence: M[+16.0]IENGSLSFLPTLR
Modification Type: Oxidation (M)
Protein - Site of Modification: 268
Peptide - Site of Modification: 1
Peptide Start: 268
Peptide End: 281

CPTAC ID: CPTAC-3273
Peptide Molecular Mass: 1,592.8232
Species: Canis lupus familiaris (Dog)
Assay Type: Direct MRM
Matrix: Serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Isotope labeled peptide
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low pump
Column Packing: C18 3 µm 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	3.2	4.9	3	14.5	11.1	12.3	14.8	12.1	12.7	15	15	15
y5 (1+)	13.3	7.2	5.1	17.5	10.8	12.1	22	13	13.1	15	15	15
y7 (1+)	9	3.3	4.6	19.8	9.7	15.5	21.7	10.2	16.2	15	15	15
sum	3.1	3	2.7	14.6	9.9	12.8	14.9	10.3	13.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3278 Collapse assay details

Data source: Panorama

Official Gene Symbol: BGN
Peptide Modified Sequence: M[+16.0]IENGSLSFLPTLR
Modification Type: Oxidation (M)
Protein - Site of Modification: 268
Peptide - Site of Modification: 1
Peptide Start: 268
Peptide End: 281

CPTAC ID: CPTAC-3278
Peptide Molecular Mass: 1,592.8232
Species: Canis lupus familiaris (Dog)
Assay Type: Direct MRM
Matrix: Tissue
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang