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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BGN N/A
Sequence Length (AA) Molecular Weight (Da)
369 41566
Protein Name
Biglycan
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWPLWLVASL LALSQALPFE QKGFWDFTLD DGLPMLNDEE ASGAETTSGV
60 70 80 90 100
PDLDALTPTY SAMCPFGCHC HLRVVQCSDL GLKAVPKEIS PDTMLLDLQN
110 120 130 140 150
NDISELRADD FKGLHHLYAL VLVNNKISKI HEKAFSPLRK LQKLYISKNH
160 170 180 190 200
LVEIPPNLPS SLVELRIHDN RIRKVPKGVF SGLRNMNCIE MGGNPLENSG
210 220 230 240 250
FEPGAFDGLK LNYLRISEAK LTGIPKDLPE TLNELHLDHN KIQAIELEDL
260 270 280 290 300
LRYSKLYRLG LGHNQIRMIE NGSLSFLPTL RELHLDNNKL SRVPSGLPDL
310 320 330 340 350
KLLQVVYLHT NNITKVGVND FCPVGFGVKR AYYNGISLFN NPVPYWEVQP
360 369
ATFRCVTDRL AIQFGNYKK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3273 Collapse assay details

Data source: Panorama

Official Gene Symbol
BGN
Peptide Modified Sequence
M[+16.0]IENGSLSFLPTLR
Modification Type
Oxidation (M)
Protein - Site of Modification
268
Peptide - Site of Modification
1
Peptide Start
268
Peptide End
281
CPTAC ID
CPTAC-3273
Peptide Molecular Mass
1,592.8232
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 3.2 4.9 3 14.5 11.1 12.3 14.8 12.1 12.7 15 15 15
y5 (1+) 13.3 7.2 5.1 17.5 10.8 12.1 22 13 13.1 15 15 15
y7 (1+) 9 3.3 4.6 19.8 9.7 15.5 21.7 10.2 16.2 15 15 15
sum 3.1 3 2.7 14.6 9.9 12.8 14.9 10.3 13.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3278 Collapse assay details

Data source: Panorama

Official Gene Symbol
BGN
Peptide Modified Sequence
M[+16.0]IENGSLSFLPTLR
Modification Type
Oxidation (M)
Protein - Site of Modification
268
Peptide - Site of Modification
1
Peptide Start
268
Peptide End
281
CPTAC ID
CPTAC-3278
Peptide Molecular Mass
1,592.8232
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.8 3.3 4.4 4.5 3.5 7.4 5.9 4.8 8.6 15 15 15
y5 (1+) 10.1 4.9 6.1 8.4 4.7 6.1 13.1 6.8 8.6 15 15 15
y4 (1+) 4.4 2.8 3.5 5.3 3.3 4.7 6.9 4.3 5.9 15 15 15
sum 3.7 1.8 1.4 4.1 2.6 3.8 5.5 3.2 4 15 15 15


Additional Resources and Comments