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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PEBP1 PBP, PEBP
Sequence Length (AA) Molecular Weight (Da)
187 21057
Protein Name
Phosphatidylethanolamine-binding protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP
60 70 80 90 100
TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK
160 170 180 187
VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-423 Collapse assay details

Data source: Panorama

Official Gene Symbol
PEBP1
Peptide Sequence
NRPTSISWDGLDSGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
48
Peptide End
62
CPTAC ID
CPTAC-423
Peptide Molecular Mass
1,631.7903
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.7 8 4.6 12.6 7.5 13.8 14.8 11 14.5 15 25 15
y7 (1+) 5.9 7.1 5.3 8 7.7 6.3 9.9 10.5 8.2 15 25 15
y6 (1+) 7.1 6.9 5.2 4.5 7.4 8.4 8.4 10.1 9.9 15 25 15
sum 5.5 6.5 4.7 6.1 7 8.1 8.2 9.6 9.4 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-424 Collapse assay details

Data source: Panorama

Official Gene Symbol
PEBP1
Peptide Sequence
YVWLVYEQDRPLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
120
Peptide End
132
CPTAC ID
CPTAC-424
Peptide Molecular Mass
1,707.8984
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 28.7 32.7 24.7 63.3 65.5 38.1 69.5 73.2 45.4 15 25 15
y11 (2+) 10.8 10.9 7.4 22.2 17.1 8.4 24.7 20.3 11.2 15 25 15
y10 (2+) 9 10.5 6.1 23.1 21.7 7.9 24.8 24.1 10 15 25 15
sum 9.1 10.3 7 20.3 17.6 7.4 22.2 20.4 10.2 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-1568 Collapse assay details

Data source: Panorama

Official Gene Symbol
PEBP1
Peptide Sequence
VLTPTQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
40
Peptide End
47
CPTAC ID
CPTAC-1568
Peptide Molecular Mass
884.5331
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.9 2.4 1.9 9.2 3.2 6.7 12.8 4 7 15 15 15
y5 (1+) 9.7 4.2 2.6 11.6 4.9 6.2 15.1 6.5 6.7 15 15 15
y6 (2+) 16.3 3.9 3.3 13.6 7 6.2 21.2 8 7 15 15 15
b2 (1+) 14 3.8 3.3 13.8 6.9 5.2 19.7 7.9 6.2 15 15 15
sum 3.9 2.7 1.8 4.4 4.6 5.8 5.9 5.3 6.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3995 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pebp1
Peptide Sequence
LYEQLSGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
180
Peptide End
187
CPTAC ID
non-CPTAC-3995
Peptide Molecular Mass
936.4916
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.7 3.7 5 10.5 4.8 3.8 15 6.1 6.3 15 15 15
y2 (1+) 18 7.7 7.5 21.5 7.8 8.6 28 11 11.4 15 15 15
y3 (1+) 11.3 6.5 3.4 21 10.9 4.8 23.8 12.7 5.9 15 15 15
y6 (1+) 8.9 5.1 3.1 12.1 6.3 4.4 15 8.1 5.4 15 15 15
y7 (1+) 9.3 4.8 4.3 18.3 8.9 6.5 20.5 10.1 7.8 15 15 15
sum 5.8 2.7 2 9.8 5.3 3.5 11.4 5.9 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3731 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pebp1
Peptide Sequence
VDYAGVTVDELGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
27
Peptide End
39
CPTAC ID
non-CPTAC-3731
Peptide Molecular Mass
1,364.6824
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 21.2 6.7 6.9 20.5 8.2 7.7 29.5 10.6 10.3 15 15 15
b3 (1+) 8 7 4.8 12.7 8.3 10.6 15 10.9 11.6 15 15 15
y11 (2+) 9.4 5.1 4.4 11.4 5.7 8.1 14.8 7.6 9.2 15 15 15
y7 (1+) 8.6 6.7 8.4 11.6 9.3 6.5 14.4 11.5 10.6 15 15 15
y9 (1+) 9.4 8.5 4.8 15.9 8.8 12.2 18.5 12.2 13.1 15 15 15
sum 6.1 2.2 2.4 10 3.3 6.8 11.7 4 7.2 15 15 15


Additional Resources and Comments