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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ARG1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ARG1 N/A
Sequence Length (AA) Molecular Weight (Da)
322 34735
Protein Name
Arginase-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY
60 70 80 90 100
GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD
110 120 130 140 150
HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK
160 170 180 190 200
ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM
210 220 230 240 250
TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG
260 270 280 290 300
GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT
310 320 322
LACFGLAREG NHKPIDYLNP PK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-6041 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARG1
Peptide Sequence
DVDPGEHYILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
181
Peptide End
191
CPTAC ID
CPTAC-6041
Peptide Molecular Mass
1,284.6350
Species
Human
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE lung tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.075 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 32.7 22.3 18 40.3 27.6 26.2 51.9 35.5 31.8 15 15 15
y5 (1+) 50.3 36.6 28 54.7 42.4 27.7 74.3 56 39.4 15 15 15
y7 (1+) 33 18.3 17.4 44.3 33.8 26.8 55.2 38.4 32 15 15 15
y8 (2+) 25.5 12.8 11 28.7 22.8 15.7 38.4 26.1 19.2 15 15 15
y9 (2+) 17.5 17.1 15.4 25.1 24.9 18.4 30.6 30.2 24 15 15 15
sum 20.5 12.8 11.7 25.2 22.3 16.1 32.5 25.7 19.9 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3893 Collapse assay details

Data source: Panorama

Official Gene Symbol
Arg1
Peptide Sequence
ANEELAGVVAEVQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
76
Peptide End
89
CPTAC ID
non-CPTAC-3893
Peptide Molecular Mass
1,455.7569
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 8.5 4.2 5.7 15.2 9.5 6.3 17.4 10.4 8.5 15 15 15
y8 (1+) 11.4 5.4 4.6 19.7 8.4 6.8 22.8 10 8.2 15 15 15
y6 (1+) 14.2 9.8 6.1 17.4 11.4 7.1 22.5 15 9.4 15 15 15
b4 (1+) 24.8 8.7 11.3 25.4 12.5 11.6 35.5 15.2 16.2 15 15 15
b5 (1+) 11.9 12.4 4.5 22 12.8 7.7 25 17.8 8.9 15 15 15
sum 8.9 3.5 3.3 16.3 7.6 4.4 18.6 8.4 5.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments