CDK1, CPTAC-797 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK1	CDC2,CDC28A,CDKN1,P34CDC2

Sequence Length (AA)	Molecular Weight (Da)
297	34095

Protein Name
Cyclin-dependent kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEDYTKIEKI	GEGTYGVVYK	GRHKTTGQVV	AMKKIRLESE	EEGVPSTAIR
60	70	80	90	100
EISLLKELRH	PNIVSLQDVL	MQDSRLYLIF	EFLSMDLKKY	LDSIPPGQYM
110	120	130	140	150
DSSLVKSYLY	QILQGIVFCH	SRRVLHRDLK	PQNLLIDDKG	TIKLADFGLA
160	170	180	190	200
RAFGIPIRVY	THEVVTLWYR	SPEVLLGSAR	YSTPVDIWSI	GTIFAELATK
210	220	230	240	250
KPLFHGDSEI	DQLFRIFRAL	GTPNNEVWPE	VESLQDYKNT	FPKWKPGSLA
260	270	280	290	297
SHVKNLDENG	LDLLSKMLIY	DPAKRISGKM	ALNHPYFNDL	DNQIKKM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1200 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Sequence: LESEEEGVPSTAIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 37
Peptide End: 50

CPTAC ID: CPTAC-1200
Peptide Molecular Mass: 1,515.7417
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	18	6.7	4.4	22	7.1	5.6	28.4	9.8	7.1	15	15	15
y8 (1+)	27.4	8.4	3.3	30.3	13.3	3.6	40.9	15.7	4.9	15	15	15
y9 (1+)	35.3	12.9	6.6	48.6	13.3	9.5	60.1	18.5	11.6	15	15	15
sum	14.3	5.1	3.3	20.9	7.1	4.2	25.3	8.7	5.3	15	15	15

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Assay Details for CPTAC-797 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Modified Sequence: IGEGTY[+80.0]GVVYK
Modification Type: Phospho (Y)
Protein - Site of Modification: 15
Peptide - Site of Modification: 6
Peptide Start: 10
Peptide End: 20

CPTAC ID: CPTAC-797
Peptide Molecular Mass: 1,264.5741
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	14.8	8.2	10.4	16.1	12.1	10.2	21.9	14.6	14.6	15	15	15
y5 (1+)	15.7	15.6	7.5	18.9	15.3	7.6	24.6	21.9	10.7	15	15	15
y6 (1+)	15.2	12.9	15	15.9	12.6	13.3	22	18	20	15	15	15
y7 (1+)	32.8	18.3	9.5	31.7	18.7	14.7	45.6	26.2	17.5	15	15	15
y8 (1+)	16.1	6.7	6.6	11.4	13	7.4	19.7	14.6	9.9	15	15	15
y10 (1+)	19.3	10.4	5.8	21.1	10.5	7.2	28.6	14.8	9.2	15	15	15
sum	7.4	4.1	5.6	7.7	5.6	6.1	10.7	6.9	8.3	15	15	15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-798 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Sequence: IGEGTYGVVYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 10
Peptide End: 20

CPTAC ID: CPTAC-798
Peptide Molecular Mass: 1,184.6077
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	11.7	4.8	3.8	10.4	4.4	3.6	15.7	6.5	5.2	15	15	15
y4 (1+)	18.1	11.4	10.2	23.6	13.5	12.4	29.7	17.7	16.1	15	15	15
y5 (1+)	10.7	4.2	5.7	10.6	4.6	5.3	15.1	6.2	7.8	15	15	15
y6 (1+)	10.5	9.9	8.7	11.4	12.8	9	15.5	16.2	12.5	15	15	15
y7 (1+)	21.6	11.6	19.3	24.7	11.9	14.9	32.8	16.6	24.4	15	15	15
y8 (1+)	11.9	5.2	8.2	12.9	6.4	8.3	17.6	8.2	11.7	15	15	15
y9 (1+)	11.7	8.7	9.4	12.6	10.5	9.4	17.2	13.6	13.3	15	15	15
y10 (1+)	9.6	5.4	3.3	12.2	4.9	3.2	15.5	7.3	4.6	15	15	15
sum	5.3	3.1	3.3	6.6	2.4	3.1	8.5	3.9	4.5	15	15	15

Additional Resources and Comments

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Assay Details for CPTAC-1040 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Modified Sequence: IGEGT[+80.0]YGVVYK
Modification Type: Phospho (ST)
Protein - Site of Modification: 14
Peptide - Site of Modification: 5
Peptide Start: 10
Peptide End: 20

CPTAC ID: CPTAC-1040
Peptide Molecular Mass: 1,264.5741
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	37.4	40.1	55.9	51.1	37.4	83	63.3	54.8	100.1	15	15	15
y5 (1+)	19.1	9.8	6.8	20.7	11.1	7.8	28.2	14.8	10.3	15	15	15
y4 (1+)	25.1	15.9	7.5	27.2	16	15.9	37	22.6	17.6	15	15	15
sum	14.3	8.8	4.8	14.8	9.3	7.1	20.6	12.8	8.6	15	15	15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-801 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Sequence: VYTHEVVTLWYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 159
Peptide End: 170

CPTAC ID: CPTAC-801
Peptide Molecular Mass: 1,564.8038
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	12.3	4.4	4	10.3	4.7	4	16	6.4	5.7	15	15	15
y6 (1+)	10.5	12.1	11.1	9.2	12.8	11.5	14	17.6	16	15	15	15
y7 (1+)	11.6	5.9	9.2	16.3	6.9	10.9	20	9.1	14.3	15	15	15
y8 (1+)	9.4	6.5	11.4	10.5	9.6	11.3	14.1	11.6	16.1	15	15	15
b6 (1+)	8.4	9	8.6	15.6	9.6	9.8	17.7	13.2	13	15	15	15
b5 (1+)	12.4	4.8	5.7	12.4	4.9	6.1	17.5	6.9	8.3	15	15	15
sum	5.7	3.3	2.2	5	3	2.1	7.6	4.5	3	15	15	15

Additional Resources and Comments

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Assay Details for CPTAC-1041 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Modified Sequence: VY[+80.0]THEVVTLWYR
Modification Type: Phospho (Y)
Protein - Site of Modification: 160
Peptide - Site of Modification: 2
Peptide Start: 159
Peptide End: 170

CPTAC ID: CPTAC-1041
Peptide Molecular Mass: 1,644.7701
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	44.3	9.7	12.6	56.2	5.7	11.2	71.6	11.3	16.9	15	15	15
y4 (1+)	34.5	16.6	16.6	41.1	15.3	12.5	53.7	22.6	20.8	15	15	15
y3 (1+)	19	14.5	14.8	32.3	13.5	14.8	37.5	19.8	20.9	15	15	15
sum	23.1	8.6	7.1	28.7	7.4	7.2	36.8	11.3	10.1	15	15	15

Additional Resources and Comments

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Assay Details for CPTAC-1042 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Modified Sequence: VYT[+80.0]HEVVTLWYR
Modification Type: Phospho (ST)
Protein - Site of Modification: 161
Peptide - Site of Modification: 3
Peptide Start: 159
Peptide End: 170

CPTAC ID: CPTAC-1042
Peptide Molecular Mass: 1,644.7701
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	39.6	11.5	13.3	45.8	14	18.3	60.5	18.1	22.6	15	15	15
y4 (1+)	36.1	33.7	12.7	55.1	33.9	12.2	65.9	47.8	17.6	15	15	15
y3 (1+)	48	17.5	14.3	45.8	20.1	16.2	66.3	26.7	21.6	15	15	15
sum	18.2	12.2	7.8	27.2	12.6	11.7	32.7	17.5	14.1	15	15	15

Additional Resources and Comments

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