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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK1 CDC2, CDC28A, CDKN1, P34CDC2
Sequence Length (AA) Molecular Weight (Da)
297 34095
Protein Name
Cyclin-dependent kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR
60 70 80 90 100
EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM
110 120 130 140 150
DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA
160 170 180 190 200
RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA
260 270 280 290 297
SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5897 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Sequence
VYTHEVVTLWYR
Modification Type
unmodified
Protein - Site of Modification
159
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-5897
Peptide Molecular Mass
1,564.8038
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 24.9 11.3 7.9 24.6 21.2 7.5 35 24 10.9 15 27 15
b7 (1+) 25.3 13.3 5.9 31.5 24.9 9.1 40.4 28.2 10.8 15 27 15
b6 (1+) 13.9 15 9 30.7 21.9 9.1 33.7 26.5 12.8 15 27 15
y10 (2+) 31.5 14.7 9.8 37.3 25.8 6.5 48.8 29.7 11.8 15 27 15
y11 (2+) 20.5 13.4 12 24.6 22.1 8.5 32 25.8 14.7 15 27 15
y3 (1+) 18.2 9.3 8 17.6 18.7 8.4 25.3 20.9 11.6 15 27 15
y5 (1+) 20.7 12.5 8 20 18.4 8.7 28.8 22.2 11.8 15 27 15
sum 11.8 9.3 7.7 13.7 19.8 6.9 18.1 21.9 10.3 15 27 15


Additional Resources and Comments


Assay Details for CPTAC-5898 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Modified Sequence
VYT[+79.966331]HEVVTLWYR
Modification Type
Phospho (ST)
Protein - Site of Modification
162
Peptide - Site of Modification
3
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-5898
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (2+) 15.3 8.7 5.4 24.1 19.6 5.7 28.5 21.4 7.9 15 27 15
b7 (1+) 18.8 9.4 5.1 19.2 19.2 7.1 26.9 21.4 8.7 15 27 15
b6 (1+) 17.9 8.6 4 17 21.2 6.3 24.7 22.9 7.5 15 27 15
y3 (1+) 10.9 6.6 4.5 14.1 19.4 10 17.8 20.5 11 15 27 15
y4 (1+) 16.8 9.9 5.2 18.9 19 8 25.3 21.4 9.5 15 27 15
y5 (1+) 14.4 6.9 4.1 17.3 17.1 9.4 22.5 18.4 10.3 15 27 15
y6 (1+) 23.9 8.9 4.4 23.9 14.8 7.7 33.8 17.3 8.9 15 27 15
sum 7.4 5.5 2.8 10.1 17.9 7 12.5 18.7 7.5 15 27 15


Additional Resources and Comments


Assay Details for CPTAC-1200 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Sequence
LESEEEGVPSTAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
37
Peptide End
50
CPTAC ID
CPTAC-1200
Peptide Molecular Mass
1,515.7417
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 18 6.7 4.4 22 7.1 5.6 28.4 9.8 7.1 15 15 15
y8 (1+) 27.4 8.4 3.3 30.3 13.3 3.6 40.9 15.7 4.9 15 15 15
y9 (1+) 35.3 12.9 6.6 48.6 13.3 9.5 60.1 18.5 11.6 15 15 15
sum 14.3 5.1 3.3 20.9 7.1 4.2 25.3 8.7 5.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-797 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Modified Sequence
IGEGTY[+80.0]GVVYK
Modification Type
Phospho (Y)
Protein - Site of Modification
15
Peptide - Site of Modification
6
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-797
Peptide Molecular Mass
1,264.5741
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.8 8.2 10.4 16.1 12.1 10.2 21.9 14.6 14.6 15 15 15
y5 (1+) 15.7 15.6 7.5 18.9 15.3 7.6 24.6 21.9 10.7 15 15 15
y6 (1+) 15.2 12.9 15 15.9 12.6 13.3 22 18 20 15 15 15
y7 (1+) 32.8 18.3 9.5 31.7 18.7 14.7 45.6 26.2 17.5 15 15 15
y8 (1+) 16.1 6.7 6.6 11.4 13 7.4 19.7 14.6 9.9 15 15 15
y10 (1+) 19.3 10.4 5.8 21.1 10.5 7.2 28.6 14.8 9.2 15 15 15
sum 7.4 4.1 5.6 7.7 5.6 6.1 10.7 6.9 8.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-798 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Sequence
IGEGTYGVVYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-798
Peptide Molecular Mass
1,184.6077
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 11.7 4.8 3.8 10.4 4.4 3.6 15.7 6.5 5.2 15 15 15
y4 (1+) 18.1 11.4 10.2 23.6 13.5 12.4 29.7 17.7 16.1 15 15 15
y5 (1+) 10.7 4.2 5.7 10.6 4.6 5.3 15.1 6.2 7.8 15 15 15
y6 (1+) 10.5 9.9 8.7 11.4 12.8 9 15.5 16.2 12.5 15 15 15
y7 (1+) 21.6 11.6 19.3 24.7 11.9 14.9 32.8 16.6 24.4 15 15 15
y8 (1+) 11.9 5.2 8.2 12.9 6.4 8.3 17.6 8.2 11.7 15 15 15
y9 (1+) 11.7 8.7 9.4 12.6 10.5 9.4 17.2 13.6 13.3 15 15 15
y10 (1+) 9.6 5.4 3.3 12.2 4.9 3.2 15.5 7.3 4.6 15 15 15
sum 5.3 3.1 3.3 6.6 2.4 3.1 8.5 3.9 4.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1040 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Modified Sequence
IGEGT[+80.0]YGVVYK
Modification Type
Phospho (ST)
Protein - Site of Modification
14
Peptide - Site of Modification
5
Peptide Start
10
Peptide End
20
CPTAC ID
CPTAC-1040
Peptide Molecular Mass
1,264.5741
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 37.4 40.1 55.9 51.1 37.4 83 63.3 54.8 100.1 15 15 15
y5 (1+) 19.1 9.8 6.8 20.7 11.1 7.8 28.2 14.8 10.3 15 15 15
y4 (1+) 25.1 15.9 7.5 27.2 16 15.9 37 22.6 17.6 15 15 15
sum 14.3 8.8 4.8 14.8 9.3 7.1 20.6 12.8 8.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3054 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Sequence
LESEEEGVPSTAIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
37
Peptide End
50
CPTAC ID
CPTAC-3054
Peptide Molecular Mass
1,515.7417
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.9 2.4 1.2 4.6 2.6 1.9 6.7 3.5 2.2 15 15 15
y8 (1+) 3.7 2.6 2.2 4.3 2.7 2.3 5.7 3.7 3.2 15 15 15
y9 (1+) 6 2.5 2.3 6.3 3.1 2.9 8.7 4 3.7 15 15 15
sum 4.6 2.4 1.3 4.5 2.6 1.9 6.4 3.5 2.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-801 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Sequence
VYTHEVVTLWYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-801
Peptide Molecular Mass
1,564.8038
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.3 4.4 4 10.3 4.7 4 16 6.4 5.7 15 15 15
y6 (1+) 10.5 12.1 11.1 9.2 12.8 11.5 14 17.6 16 15 15 15
y7 (1+) 11.6 5.9 9.2 16.3 6.9 10.9 20 9.1 14.3 15 15 15
y8 (1+) 9.4 6.5 11.4 10.5 9.6 11.3 14.1 11.6 16.1 15 15 15
b6 (1+) 8.4 9 8.6 15.6 9.6 9.8 17.7 13.2 13 15 15 15
b5 (1+) 12.4 4.8 5.7 12.4 4.9 6.1 17.5 6.9 8.3 15 15 15
sum 5.7 3.3 2.2 5 3 2.1 7.6 4.5 3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1041 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Modified Sequence
VY[+80.0]THEVVTLWYR
Modification Type
Phospho (Y)
Protein - Site of Modification
160
Peptide - Site of Modification
2
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-1041
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 44.3 9.7 12.6 56.2 5.7 11.2 71.6 11.3 16.9 15 15 15
y4 (1+) 34.5 16.6 16.6 41.1 15.3 12.5 53.7 22.6 20.8 15 15 15
y3 (1+) 19 14.5 14.8 32.3 13.5 14.8 37.5 19.8 20.9 15 15 15
sum 23.1 8.6 7.1 28.7 7.4 7.2 36.8 11.3 10.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1042 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK1
Peptide Modified Sequence
VYT[+80.0]HEVVTLWYR
Modification Type
Phospho (ST)
Protein - Site of Modification
161
Peptide - Site of Modification
3
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-1042
Peptide Molecular Mass
1,644.7701
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 39.6 11.5 13.3 45.8 14 18.3 60.5 18.1 22.6 15 15 15
y4 (1+) 36.1 33.7 12.7 55.1 33.9 12.2 65.9 47.8 17.6 15 15 15
y3 (1+) 48 17.5 14.3 45.8 20.1 16.2 66.3 26.7 21.6 15 15 15
sum 18.2 12.2 7.8 27.2 12.6 11.7 32.7 17.5 14.1 15 15 15


Additional Resources and Comments