CDK1, CPTAC-797 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK1	CDC2, CDC28A, CDKN1, P34CDC2

Sequence Length (AA)	Molecular Weight (Da)
297	34095

Protein Name
Cyclin-dependent kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEDYTKIEKI	GEGTYGVVYK	GRHKTTGQVV	AMKKIRLESE	EEGVPSTAIR
60	70	80	90	100
EISLLKELRH	PNIVSLQDVL	MQDSRLYLIF	EFLSMDLKKY	LDSIPPGQYM
110	120	130	140	150
DSSLVKSYLY	QILQGIVFCH	SRRVLHRDLK	PQNLLIDDKG	TIKLADFGLA
160	170	180	190	200
RAFGIPIRVY	THEVVTLWYR	SPEVLLGSAR	YSTPVDIWSI	GTIFAELATK
210	220	230	240	250
KPLFHGDSEI	DQLFRIFRAL	GTPNNEVWPE	VESLQDYKNT	FPKWKPGSLA
260	270	280	290	297
SHVKNLDENG	LDLLSKMLIY	DPAKRISGKM	ALNHPYFNDL	DNQIKKM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-5897 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK1
Peptide Sequence: VYTHEVVTLWYR
Modification Type: unmodified
Protein - Site of Modification: 159
Peptide - Site of Modification: N/A
Peptide Start: 159
Peptide End: 170

CPTAC ID: CPTAC-5897
Peptide Molecular Mass: 1,564.8038
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: N/A
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Sciex 5500 QTRAP
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent 425
Column Packing: Reprosil
Column Dimensions: 75um x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	24.9	11.3	7.9	24.6	21.2	7.5	35	24	10.9	15	27	15
b7 (1+)	25.3	13.3	5.9	31.5	24.9	9.1	40.4	28.2	10.8	15	27	15
b6 (1+)	13.9	15	9	30.7	21.9	9.1	33.7	26.5	12.8	15	27	15
y10 (2+)	31.5	14.7	9.8	37.3	25.8	6.5	48.8	29.7	11.8	15	27	15
y11 (2+)	20.5	13.4	12	24.6	22.1	8.5	32	25.8	14.7	15	27	15
y3 (1+)	18.2	9.3	8	17.6	18.7	8.4	25.3	20.9	11.6	15	27	15
y5 (1+)	20.7	12.5	8	20	18.4	8.7	28.8	22.2	11.8	15	27	15
sum	11.8	9.3	7.7	13.7	19.8	6.9	18.1	21.9	10.3	15	27	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (2+)	15.3	8.7	5.4	24.1	19.6	5.7	28.5	21.4	7.9	15	27	15
b7 (1+)	18.8	9.4	5.1	19.2	19.2	7.1	26.9	21.4	8.7	15	27	15
b6 (1+)	17.9	8.6	4	17	21.2	6.3	24.7	22.9	7.5	15	27	15
y3 (1+)	10.9	6.6	4.5	14.1	19.4	10	17.8	20.5	11	15	27	15
y4 (1+)	16.8	9.9	5.2	18.9	19	8	25.3	21.4	9.5	15	27	15
y5 (1+)	14.4	6.9	4.1	17.3	17.1	9.4	22.5	18.4	10.3	15	27	15
y6 (1+)	23.9	8.9	4.4	23.9	14.8	7.7	33.8	17.3	8.9	15	27	15
sum	7.4	5.5	2.8	10.1	17.9	7	12.5	18.7	7.5	15	27	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	14.8	8.2	10.4	16.1	12.1	10.2	21.9	14.6	14.6	15	15	15
y5 (1+)	15.7	15.6	7.5	18.9	15.3	7.6	24.6	21.9	10.7	15	15	15
y6 (1+)	15.2	12.9	15	15.9	12.6	13.3	22	18	20	15	15	15
y7 (1+)	32.8	18.3	9.5	31.7	18.7	14.7	45.6	26.2	17.5	15	15	15
y8 (1+)	16.1	6.7	6.6	11.4	13	7.4	19.7	14.6	9.9	15	15	15
y10 (1+)	19.3	10.4	5.8	21.1	10.5	7.2	28.6	14.8	9.2	15	15	15
sum	7.4	4.1	5.6	7.7	5.6	6.1	10.7	6.9	8.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	11.7	4.8	3.8	10.4	4.4	3.6	15.7	6.5	5.2	15	15	15
y4 (1+)	18.1	11.4	10.2	23.6	13.5	12.4	29.7	17.7	16.1	15	15	15
y5 (1+)	10.7	4.2	5.7	10.6	4.6	5.3	15.1	6.2	7.8	15	15	15
y6 (1+)	10.5	9.9	8.7	11.4	12.8	9	15.5	16.2	12.5	15	15	15
y7 (1+)	21.6	11.6	19.3	24.7	11.9	14.9	32.8	16.6	24.4	15	15	15
y8 (1+)	11.9	5.2	8.2	12.9	6.4	8.3	17.6	8.2	11.7	15	15	15
y9 (1+)	11.7	8.7	9.4	12.6	10.5	9.4	17.2	13.6	13.3	15	15	15
y10 (1+)	9.6	5.4	3.3	12.2	4.9	3.2	15.5	7.3	4.6	15	15	15
sum	5.3	3.1	3.3	6.6	2.4	3.1	8.5	3.9	4.5	15	15	15

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CDK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5897 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-5898 Collapse assay details

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Assay Details for CPTAC-1200 Collapse assay details

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Assay Details for CPTAC-797 Collapse assay details

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Assay Details for CPTAC-798 Collapse assay details

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Assay Details for CPTAC-1040 Collapse assay details

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Assay Details for CPTAC-801 Collapse assay details

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Assay Details for CPTAC-1041 Collapse assay details

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Assay Details for CPTAC-1042 Collapse assay details

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