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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ERBB2 HER2, MLN19, NEU, NGL
Sequence Length (AA) Molecular Weight (Da)
1255 137910
Protein Name
Receptor tyrosine-protein kinase erbB-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MELAALCRWG LLLALLPPGA ASTQVCTGTD MKLRLPASPE THLDMLRHLY
60 70 80 90 100
QGCQVVQGNL ELTYLPTNAS LSFLQDIQEV QGYVLIAHNQ VRQVPLQRLR
110 120 130 140 150
IVRGTQLFED NYALAVLDNG DPLNNTTPVT GASPGGLREL QLRSLTEILK
160 170 180 190 200
GGVLIQRNPQ LCYQDTILWK DIFHKNNQLA LTLIDTNRSR ACHPCSPMCK
210 220 230 240 250
GSRCWGESSE DCQSLTRTVC AGGCARCKGP LPTDCCHEQC AAGCTGPKHS
260 270 280 290 300
DCLACLHFNH SGICELHCPA LVTYNTDTFE SMPNPEGRYT FGASCVTACP
310 320 330 340 350
YNYLSTDVGS CTLVCPLHNQ EVTAEDGTQR CEKCSKPCAR VCYGLGMEHL
360 370 380 390 400
REVRAVTSAN IQEFAGCKKI FGSLAFLPES FDGDPASNTA PLQPEQLQVF
410 420 430 440 450
ETLEEITGYL YISAWPDSLP DLSVFQNLQV IRGRILHNGA YSLTLQGLGI
460 470 480 490 500
SWLGLRSLRE LGSGLALIHH NTHLCFVHTV PWDQLFRNPH QALLHTANRP
510 520 530 540 550
EDECVGEGLA CHQLCARGHC WGPGPTQCVN CSQFLRGQEC VEECRVLQGL
560 570 580 590 600
PREYVNARHC LPCHPECQPQ NGSVTCFGPE ADQCVACAHY KDPPFCVARC
610 620 630 640 650
PSGVKPDLSY MPIWKFPDEE GACQPCPINC THSCVDLDDK GCPAEQRASP
660 670 680 690 700
LTSIISAVVG ILLVVVLGVV FGILIKRRQQ KIRKYTMRRL LQETELVEPL
710 720 730 740 750
TPSGAMPNQA QMRILKETEL RKVKVLGSGA FGTVYKGIWI PDGENVKIPV
760 770 780 790 800
AIKVLRENTS PKANKEILDE AYVMAGVGSP YVSRLLGICL TSTVQLVTQL
810 820 830 840 850
MPYGCLLDHV RENRGRLGSQ DLLNWCMQIA KGMSYLEDVR LVHRDLAARN
860 870 880 890 900
VLVKSPNHVK ITDFGLARLL DIDETEYHAD GGKVPIKWMA LESILRRRFT
910 920 930 940 950
HQSDVWSYGV TVWELMTFGA KPYDGIPARE IPDLLEKGER LPQPPICTID
960 970 980 990 1000
VYMIMVKCWM IDSECRPRFR ELVSEFSRMA RDPQRFVVIQ NEDLGPASPL
1010 1020 1030 1040 1050
DSTFYRSLLE DDDMGDLVDA EEYLVPQQGF FCPDPAPGAG GMVHHRHRSS
1060 1070 1080 1090 1100
STRSGGGDLT LGLEPSEEEA PRSPLAPSEG AGSDVFDGDL GMGAAKGLQS
1110 1120 1130 1140 1150
LPTHDPSPLQ RYSEDPTVPL PSETDGYVAP LTCSPQPEYV NQPDVRPQPP
1160 1170 1180 1190 1200
SPREGPLPAA RPAGATLERP KTLSPGKNGV VKDVFAFGGA VENPEYLTPQ
1210 1220 1230 1240 1250
GGAAPQPHPP PAFSPAFDNL YYWDQDPPER GAPPSTFKGT PTAENPEYLG
1255
LDVPV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-194 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
ELVSEFSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
971
Peptide End
978
CPTAC ID
CPTAC-194
Peptide Molecular Mass
965.4818
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 7.8 6.6 6.8 9.4 15 12.8 12.2 16.4 14.5 15 25 15
y5 (1+) 10.9 5 5.7 15.3 10.8 11.2 18.8 11.9 12.6 15 25 15
y4 (1+) 9.7 5.1 8 13.6 10.5 13.5 16.7 11.7 15.7 15 25 15
sum 8.5 5.2 6.2 12.9 11.7 11.8 15.4 12.8 13.3 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-195 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GLQSLPTHDPSPLQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1097
Peptide End
1111
CPTAC ID
CPTAC-195
Peptide Molecular Mass
1,644.8584
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 8 4.2 3.6 8.8 6.9 4.8 11.9 8.1 6 15 25 15
y12 (2+) 3.3 4.4 3.7 6.9 5.9 4.3 7.6 7.4 5.7 15 25 15
y10 (2+) 3.7 5.3 3.2 6.7 6.1 4.4 7.7 8.1 5.4 15 25 15
sum 3.5 4.2 3.1 6.8 5.8 4.2 7.6 7.2 5.2 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-693 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GLQSLPTHDPSPLQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1097
Peptide End
1111
CPTAC ID
CPTAC-693
Peptide Molecular Mass
1,644.8584
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 15.4 3.5 1.9 21.9 4.1 4.4 26.8 5.4 4.8 15 15 15
y4 (1+) 22.6 5.5 2.4 26.3 6.8 4.9 34.7 8.7 5.5 15 15 15
y6 (1+) 16.8 4.9 2.8 18 6.9 5 24.6 8.5 5.7 15 15 15
sum 13.6 2.8 1.4 18.5 4.2 4.3 23 5 4.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5839 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GLQSLPTHDPSPLQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
1097
Peptide End
1111
CPTAC ID
CPTAC-5839
Peptide Molecular Mass
1,644.8584
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 14.6 4.6 3.3 15 4.9 4.1 20.9 6.7 5.3 15 17 15
y13 (2+) 12.8 6.8 3.5 16.9 8.1 5.3 21.2 10.6 6.4 15 17 15
y12 (2+) 11.1 3.8 3.7 8.9 5.1 4.1 14.2 6.4 5.5 15 17 15
y10 (2+) 17.4 4.4 3.7 16.6 4.9 4.8 24 6.6 6.1 15 17 15
sum 11.5 3.3 2.7 11.5 4.6 4.1 16.3 5.7 4.9 15 17 15


Additional Resources and Comments


Assay Details for CPTAC-5864 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GLQSLPTHDPSPLQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
1097
Peptide End
1111
CPTAC ID
CPTAC-5864
Peptide Molecular Mass
1,644.8584
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 16.2 5.2 3.9 17.2 9.3 4.3 23.6 10.7 5.8 12 12 12
y13 (2+) 22.2 9.7 3.8 16.8 12.8 3.6 27.8 16.1 5.2 12 12 12
y6 (1+) 17.3 5.9 3.6 13.4 6.2 3.6 21.9 8.6 5.1 12 12 12
y10 (2+) 16 3.8 5.5 13.2 5.9 4.3 20.7 7 7 12 12 12
sum 16.7 4.2 3.6 13.5 7 3 21.5 8.2 4.7 12 12 12


Additional Resources and Comments


Assay Details for CPTAC-193 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
LLDIDETEYHADGGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
869
Peptide End
883
CPTAC ID
CPTAC-193
Peptide Molecular Mass
1,674.7737
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 29.9 22.1 10.9 21.6 28.9 18.4 36.9 36.4 21.4 15 25 15
y13 (2+) 8.5 6.1 7.3 21.4 18.2 22.9 23 19.2 24 15 25 15
y12 (2+) 20.7 15.2 15.4 23.7 23.5 25.6 31.5 28 29.9 15 25 15
sum 8.3 6.8 7.9 20.4 18 22.3 22 19.2 23.7 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-192 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Modified Sequence
NPQLC[+57.0]YQDTILWK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
162
Peptide - Site of Modification
5
Peptide Start
158
Peptide End
170
CPTAC ID
CPTAC-192
Peptide Molecular Mass
1,677.8185
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 15.2 9.4 10.7 15.3 10.7 15.6 21.6 14.2 18.9 25 15 15
y8 (1+) 15.5 8.7 5.7 17.3 7.1 9.2 23.2 11.2 10.8 25 15 15
y9 (1+) 11.6 8 4.4 12.4 7.2 11 17 10.8 11.8 25 15 15
sum 9.3 7.3 5.4 10.1 5.9 9.9 13.7 9.4 11.3 25 15 15


Additional Resources and Comments


Assay Details for CPTAC-5777 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GIWIPDGENVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
737
Peptide End
747
CPTAC ID
CPTAC-5777
Peptide Molecular Mass
1,226.6295
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 40.7 12 14.8 39.2 26.6 19 56.5 29.2 24.1 15 15 15
y7 (1+) 29.1 10.5 13.7 23.9 28.1 19.3 37.7 30 23.7 15 15 15
y5 (1+) 57 15.1 16.4 74 30.1 18.7 93.4 33.7 24.9 15 15 15
sum 38.2 9.8 14.1 34.4 27.4 19.1 51.4 29.1 23.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5827 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Modified Sequence
GTPTAENPEY[+79.966331]LGLDVPV
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1239
Peptide End
1255
CPTAC ID
CPTAC-5827
Peptide Molecular Mass
1,850.8339
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (1+) 17.2 13.7 6.1 21.1 15.7 6.7 27.2 20.8 9.1 16 15 13
b7 (1+) 16.8 13.7 9.3 20.2 15.5 7.1 26.3 20.7 11.7 16 15 13
sum 16.4 13.4 7.6 18.1 15.5 6.8 24.4 20.5 10.2 16 15 13


Additional Resources and Comments


Assay Details for non-CPTAC-5385 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
ELVSEFSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
971
Peptide End
978
CPTAC ID
non-CPTAC-5385
Peptide Molecular Mass
965.4818
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 3.9 1.6 3.5 4.5 2.9 3.2 6 3.3 4.7 15 15 15
y4 (1+) 9.2 3 3 8.7 5.7 3.6 12.7 6.4 4.7 15 15 15
y5 (1+) 3.1 1.8 2.8 3 3.1 2.7 4.3 3.6 3.9 15 15 15
y6 (1+) 7.2 3.7 2.4 7.5 3.8 3 10.4 5.3 3.8 15 15 15
sum 2.9 1.5 2.1 3 3 2.2 4.2 3.4 3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5547 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GIWIPDGENVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
737
Peptide End
747
CPTAC ID
non-CPTAC-5547
Peptide Molecular Mass
1,226.6295
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 44.7 13 12.1 39.2 32.4 19.4 59.5 34.9 22.9 11 11 11
y5 (1+) 57 13.3 14.4 67.7 37.5 20.2 88.5 39.8 24.8 11 11 11
y7 (1+) 32.8 12.5 11.4 23.3 34.4 19.2 40.2 36.6 22.3 11 11 11
y8 (1+) 41.1 13.8 10.3 37.1 33.2 17.1 55.4 36 20 11 11 11
y9 (1+) 40.5 15.6 12.7 35.8 35.5 18.7 54.1 38.8 22.6 11 11 11
sum 42.8 11.1 11.7 34.7 33.8 19.1 55.1 35.6 22.4 11 11 11


Additional Resources and Comments


Assay Details for non-CPTAC-5386 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
GTPTAENPEYLGLDVPV
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1239
Peptide End
1255
CPTAC ID
non-CPTAC-5386
Peptide Molecular Mass
1,770.8676
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b14 (1+) 45.2 14.4 70.4 45.3 16.4 76.4 64 21.8 103.9 15 15 15
b7 (1+) 38.5 14.8 11 39.1 15 11.4 54.9 21.1 15.8 15 15 15
b6 (1+) 23.9 18.5 7 29.3 22 4.9 37.8 28.7 8.5 15 15 15
sum 15.9 13.8 9.9 17.5 13.2 9.8 23.6 19.1 13.9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5732 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Modified Sequence
GTPTAENPEY[+79.966331]LGLDVPV
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1239
Peptide End
1255
CPTAC ID
non-CPTAC-5732
Peptide Molecular Mass
1,850.8339
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 6.9 5.8 2.7 8.2 5.7 2.7 10.7 8.1 3.8 15 15 15
b6 (1+) 4.8 4.2 2.8 5.6 4.7 3 7.4 6.3 4.1 15 15 15
y3 (1+) 3.9 2.4 1.7 3.4 3.8 3.2 5.2 4.5 3.6 15 15 15
sum 3.1 1.8 1.3 2.6 2.3 1.7 4 2.9 2.1 15 15 15
b11 (1+) 5.8 4.9 2.4 5.8 3.9 3.6 8.2 6.3 4.3 15 15 15
b10 (1+) 6.3 5.3 3 6.1 5.2 3.5 8.8 7.4 4.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5384 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Sequence
LLDIDETEYHADGGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
869
Peptide End
883
CPTAC ID
non-CPTAC-5384
Peptide Molecular Mass
1,674.7737
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.1 6.2 2 11.3 7.5 3 15.8 9.7 3.6 15 15 15
y8 (1+) 17.6 6.4 3.3 18.9 8.2 3.5 25.8 10.4 4.8 15 15 15
y9 (1+) 7.5 5.1 7.1 8.2 6 7.9 11.1 7.9 10.6 15 15 15
y5 (1+) 10.6 5.2 4.7 12.8 4.1 4.1 16.6 6.6 6.2 15 15 15
y6 (1+) 17.4 5.1 4 15 4.4 4.1 23 6.7 5.7 15 15 15
sum 6.2 4.6 3 7.1 4.1 3.2 9.4 6.2 4.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5733 Collapse assay details

Data source: Panorama

Official Gene Symbol
ERBB2
Peptide Modified Sequence
LLDIDETEY[+79.966331]HADGGK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
869
Peptide End
883
CPTAC ID
non-CPTAC-5733
Peptide Molecular Mass
1,754.7400
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 2.8 3.5 2.7 4.7 3.9 3.9 5.5 5.2 4.7 15 15 15
y13 (2+) 10.9 2.6 1.8 17 5 2.2 20.2 5.6 2.8 15 15 15
y7 (1+) 8.5 5.5 3.9 10 6 5.7 13.1 8.1 6.9 15 15 15
y9 (1+) 6.9 3.3 3 6.1 5.9 4 9.2 6.8 5 15 15 15
sum 9.3 2.3 1.3 14.3 4.4 2.2 17.1 5 2.6 15 15 15


Additional Resources and Comments