AKT3, CPTAC-2980 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AKT3	PKBG

Sequence Length (AA)	Molecular Weight (Da)
479	55775

Protein Name
RAC-gamma serine/threonine-protein kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSDVTIVKEG	WVQKRGEYIK	NWRPRYFLLK	TDGSFIGYKE	KPQDVDLPYP
60	70	80	90	100
LNNFSVAKCQ	LMKTERPKPN	TFIIRCLQWT	TVIERTFHVD	TPEEREEWTE
110	120	130	140	150
AIQAVADRLQ	RQEEERMNCS	PTSQIDNIGE	EEMDASTTHH	KRKTMNDFDY
160	170	180	190	200
LKLLGKGTFG	KVILVREKAS	GKYYAMKILK	KEVIIAKDEV	AHTLTESRVL
210	220	230	240	250
KNTRHPFLTS	LKYSFQTKDR	LCFVMEYVNG	GELFFHLSRE	RVFSEDRTRF
260	270	280	290	300
YGAEIVSALD	YLHSGKIVYR	DLKLENLMLD	KDGHIKITDF	GLCKEGITDA
310	320	330	340	350
ATMKTFCGTP	EYLAPEVLED	NDYGRAVDWW	GLGVVMYEMM	CGRLPFYNQD
360	370	380	390	400
HEKLFELILM	EDIKFPRTLS	SDAKSLLSGL	LIKDPNKRLG	GGPDDAKEIM
410	420	430	440	450
RHSFFSGVNW	QDVYDKKLVP	PFKPQVTSET	DTRYFDEEFT	AQTITITPPE
460	470	479
KYDEDGMDCM	DNERRPHFPQ	FSYSASGRE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5742 Collapse assay details

Data source: Panorama

Official Gene Symbol: AKT3
Peptide Sequence: EGITDAATMK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 295
Peptide End: 304

CPTAC ID: CPTAC-5742
Peptide Molecular Mass: 1,035.4907
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: N/A
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI: Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: SCIEX 5500 / Thermo Quantiva
Internal Standard: Stable Isotope Labeled Standard
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex Ultimate 3000
Column Packing: C18 PepMap 100
Column Dimensions: 75 micron ID x 25 cm length
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	35.7	19.1	11.7	44.5	36.1	14.7	57.1	40.8	18.8	14	15	15
y6 (1+)	63.3	11.2	26.3	50.1	40.4	27.2	80.7	41.9	37.8	14	15	15
y4 (1+)	51.4	31.1	34.6	68.6	41.5	36	85.7	51.9	49.9	14	15	15
y5 (1+)	59.4	23.3	20.1	51.3	40.8	23.9	78.5	47	31.2	14	15	15
sum	41.6	12.1	18.1	32.7	34.7	18.9	52.9	36.7	26.2	14	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	48.1	24.9	32	66.9	33.6	23.5	82.4	41.8	39.7	14	14	14
y6 (1+)	67.6	7.5	8.4	57.8	35.1	16.4	88.9	35.9	18.4	14	14	14
y7 (1+)	54.2	32.3	19	42.7	53.7	24	69	62.7	30.6	14	14	14
y8 (1+)	105.4	39.1	36.4	114.6	47.9	43.1	155.7	61.8	56.4	14	14	14
sum	32.8	9.9	5.8	41.9	30.8	13.8	53.2	32.4	15	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	49.3	23.6	20.1	32.7	37.3	15.4	59.2	44.1	25.3	10	11	11
y5 (1+)	39.3	24.1	17.5	22.3	42.8	26.5	45.2	49.1	31.8	10	11	11
y6 (1+)	33.8	9.8	27	25.7	43.6	30.8	42.5	44.7	41	10	11	11
y7 (1+)	21.3	16.9	7.2	27.5	39.8	16.1	34.8	43.2	17.6	10	11	11
sum	27.2	10.7	15.6	13.5	41.2	21.4	30.4	42.6	26.5	10	11	11

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	13.3	5.9	4.4	18.7	13.6	5.7	22.9	14.8	7.2	15	15	15
y15 (2+)	7	6.3	2.9	11.2	12.8	3.8	13.2	14.3	4.8	15	15	15
y6 (1+)	15.5	7.5	4.8	18.2	9.8	6.3	23.9	12.3	7.9	15	15	15
y7 (1+)	12.4	6.4	2.8	14.6	13.4	4.9	19.2	14.8	5.6	15	15	15
sum	6	5.7	2.5	10.7	12.6	3.5	12.3	13.8	4.3	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	2.7	3.1	2.2	4.1	3.3	2.9	4.9	4.5	3.6	13	13	12
b4 (1+)	3.8	1.5	2.1	3.6	1.8	3.1	5.2	2.3	3.7	13	13	12
b6 (2+)	4.9	3.8	3.8	6.6	3.2	3.6	8.2	5	5.2	13	13	12
y8-98 (1+)	5.5	2.1	1.6	4.9	2.6	1.4	7.4	3.3	2.1	13	13	12
y5 (1+)	4.5	2	2.1	3.9	2.6	2.2	6	3.3	3	13	13	12
sum	1.4	1.4	1.7	2.3	1.3	2.1	2.7	1.9	2.7	13	13	12

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AKT3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5742 Collapse assay details

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Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

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Assay Details for CPTAC-5789 Collapse assay details

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Assay Details for non-CPTAC-5531 Collapse assay details

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Assay Details for non-CPTAC-5342 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5344 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5693 Collapse assay details

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