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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT3 PKBG
Sequence Length (AA) Molecular Weight (Da)
479 55775
Protein Name
RAC-gamma serine/threonine-protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSDVTIVKEG WVQKRGEYIK NWRPRYFLLK TDGSFIGYKE KPQDVDLPYP
60 70 80 90 100
LNNFSVAKCQ LMKTERPKPN TFIIRCLQWT TVIERTFHVD TPEEREEWTE
110 120 130 140 150
AIQAVADRLQ RQEEERMNCS PTSQIDNIGE EEMDASTTHH KRKTMNDFDY
160 170 180 190 200
LKLLGKGTFG KVILVREKAS GKYYAMKILK KEVIIAKDEV AHTLTESRVL
210 220 230 240 250
KNTRHPFLTS LKYSFQTKDR LCFVMEYVNG GELFFHLSRE RVFSEDRTRF
260 270 280 290 300
YGAEIVSALD YLHSGKIVYR DLKLENLMLD KDGHIKITDF GLCKEGITDA
310 320 330 340 350
ATMKTFCGTP EYLAPEVLED NDYGRAVDWW GLGVVMYEMM CGRLPFYNQD
360 370 380 390 400
HEKLFELILM EDIKFPRTLS SDAKSLLSGL LIKDPNKRLG GGPDDAKEIM
410 420 430 440 450
RHSFFSGVNW QDVYDKKLVP PFKPQVTSET DTRYFDEEFT AQTITITPPE
460 470 479
KYDEDGMDCM DNERRPHFPQ FSYSASGRE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5742 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
EGITDAATMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
295
Peptide End
304
CPTAC ID
CPTAC-5742
Peptide Molecular Mass
1,035.4907
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 35.7 19.1 11.7 44.5 36.1 14.7 57.1 40.8 18.8 14 15 15
y6 (1+) 63.3 11.2 26.3 50.1 40.4 27.2 80.7 41.9 37.8 14 15 15
y4 (1+) 51.4 31.1 34.6 68.6 41.5 36 85.7 51.9 49.9 14 15 15
y5 (1+) 59.4 23.3 20.1 51.3 40.8 23.9 78.5 47 31.2 14 15 15
sum 41.6 12.1 18.1 32.7 34.7 18.9 52.9 36.7 26.2 14 15 15


Additional Resources and Comments


Assay Details for CPTAC-5788 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
RPHFPQFSYSASGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
478
CPTAC ID
CPTAC-5788
Peptide Molecular Mass
1,635.7906
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 14.4 7.5 15.5 14.1 7.5 19.5 20.2 10.6 24.9 16 15 17
y4 (1+) 14.5 8.3 15.8 13 8.9 20.2 19.5 12.2 25.6 16 15 17
sum 14.3 7.7 15.5 13.2 8 19.8 19.5 11.1 25.1 16 15 17


Additional Resources and Comments


Assay Details for CPTAC-5789 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Modified Sequence
RPHFPQFS[+79.966331]YSASGR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
478
CPTAC ID
CPTAC-5789
Peptide Molecular Mass
1,715.7570
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 8.5 10.3 21.5 15.2 14.8 27 17.4 18 34.5 16 15 16
y12 (2+) 6.9 10.2 23.2 15.1 15.3 26 16.6 18.4 34.8 16 15 16
sum 7 9.9 22.6 14.3 14.9 26.5 15.9 17.9 34.8 16 15 16


Additional Resources and Comments


Assay Details for CPTAC-5786 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
RPHFPQFSYSASGRE
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
479
CPTAC ID
CPTAC-5786
Peptide Molecular Mass
1,764.8332
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 18.6 9.7 11.8 18.6 14 16.1 26.3 17 20 16 15 17
b6 (2+) 15.1 8.8 11 17 14.5 14.8 22.7 17 18.4 16 15 17
sum 16.8 9.1 11.5 17.6 14 15.6 24.3 16.7 19.4 16 15 17


Additional Resources and Comments


Assay Details for CPTAC-5787 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Modified Sequence
RPHFPQFS[+79.966331]YSASGRE
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
479
CPTAC ID
CPTAC-5787
Peptide Molecular Mass
1,844.7995
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 9.1 11.3 8.9 15 19.1 18.1 17.5 22.2 20.2 16 15 16
b4 (1+) 9.3 10.8 9.4 17.2 18.3 17.3 19.6 21.2 19.7 16 15 16
sum 8.3 10.5 8.6 16 18.5 17 18 21.3 19.1 16 15 16


Additional Resources and Comments


Assay Details for non-CPTAC-5530 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
DEVAHTLTESR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
188
Peptide End
198
CPTAC ID
non-CPTAC-5530
Peptide Molecular Mass
1,256.5997
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 48.1 24.9 32 66.9 33.6 23.5 82.4 41.8 39.7 14 14 14
y6 (1+) 67.6 7.5 8.4 57.8 35.1 16.4 88.9 35.9 18.4 14 14 14
y7 (1+) 54.2 32.3 19 42.7 53.7 24 69 62.7 30.6 14 14 14
y8 (1+) 105.4 39.1 36.4 114.6 47.9 43.1 155.7 61.8 56.4 14 14 14
sum 32.8 9.9 5.8 41.9 30.8 13.8 53.2 32.4 15 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5531 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
EGITDAATMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
295
Peptide End
304
CPTAC ID
non-CPTAC-5531
Peptide Molecular Mass
1,035.4907
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 49.3 23.6 20.1 32.7 37.3 15.4 59.2 44.1 25.3 10 11 11
y5 (1+) 39.3 24.1 17.5 22.3 42.8 26.5 45.2 49.1 31.8 10 11 11
y6 (1+) 33.8 9.8 27 25.7 43.6 30.8 42.5 44.7 41 10 11 11
y7 (1+) 21.3 16.9 7.2 27.5 39.8 16.1 34.8 43.2 17.6 10 11 11
sum 27.2 10.7 15.6 13.5 41.2 21.4 30.4 42.6 26.5 10 11 11


Additional Resources and Comments


Assay Details for non-CPTAC-5342 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
FYGAEIVSALDYLHSGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
250
Peptide End
266
CPTAC ID
non-CPTAC-5342
Peptide Molecular Mass
1,868.9309
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.3 5.9 4.4 18.7 13.6 5.7 22.9 14.8 7.2 15 15 15
y15 (2+) 7 6.3 2.9 11.2 12.8 3.8 13.2 14.3 4.8 15 15 15
y6 (1+) 15.5 7.5 4.8 18.2 9.8 6.3 23.9 12.3 7.9 15 15 15
y7 (1+) 12.4 6.4 2.8 14.6 13.4 4.9 19.2 14.8 5.6 15 15 15
sum 6 5.7 2.5 10.7 12.6 3.5 12.3 13.8 4.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5344 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
RPHFPQFSYSASGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
465
Peptide End
478
CPTAC ID
non-CPTAC-5344
Peptide Molecular Mass
1,635.7906
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.2 4.3 2.9 12.6 5 3.2 16.2 6.6 4.3 15 15 15
y6 (1+) 12 4.8 3.3 9 7.5 4 15 8.9 5.2 15 15 15
y7 (1+) 7.5 4 2.2 7.7 4 3.6 10.7 5.7 4.2 15 15 15
sum 5.7 2.6 1.5 6.7 4.2 2.4 8.8 4.9 2.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5693 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Modified Sequence
RPHFPQFS[+79.966331]YSASGR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
478
CPTAC ID
non-CPTAC-5693
Peptide Molecular Mass
1,715.7570
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 2.7 3.1 2.2 4.1 3.3 2.9 4.9 4.5 3.6 13 13 12
b4 (1+) 3.8 1.5 2.1 3.6 1.8 3.1 5.2 2.3 3.7 13 13 12
b6 (2+) 4.9 3.8 3.8 6.6 3.2 3.6 8.2 5 5.2 13 13 12
y8-98 (1+) 5.5 2.1 1.6 4.9 2.6 1.4 7.4 3.3 2.1 13 13 12
y5 (1+) 4.5 2 2.1 3.9 2.6 2.2 6 3.3 3 13 13 12
sum 1.4 1.4 1.7 2.3 1.3 2.1 2.7 1.9 2.7 13 13 12


Additional Resources and Comments


Assay Details for non-CPTAC-5345 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
RPHFPQFSYSASGRE
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
465
Peptide End
479
CPTAC ID
non-CPTAC-5345
Peptide Molecular Mass
1,764.8332
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 16.9 10.1 5.6 15.8 9.5 5.5 23.1 13.9 7.8 15 15 15
y6 (1+) 11.3 3.4 2.8 10.8 3.5 3.4 15.6 4.9 4.4 15 15 15
y7 (1+) 17.4 4 3 19.3 5.2 4.3 26 6.6 5.2 15 15 15
y8 (1+) 11 4.1 3.2 17 4.9 3.6 20.2 6.4 4.8 15 15 15
sum 8.7 1.5 1 8.5 2.7 2.2 12.2 3.1 2.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5692 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Modified Sequence
RPHFPQFS[+79.966331]YSASGRE
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
465
Peptide End
479
CPTAC ID
non-CPTAC-5692
Peptide Molecular Mass
1,844.7995
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9-98 (1+) 5.4 3.3 1.8 5 2.7 2 7.4 4.3 2.7 13 13 13
y6 (1+) 3.1 1.9 0.9 3.7 2.2 2.3 4.8 2.9 2.5 13 13 13
y5 (1+) 4.5 1.8 2.3 5.1 2.6 1.9 6.8 3.2 3 13 13 13
y4 (1+) 6.7 3.7 2.3 6.6 3.8 2.6 9.4 5.3 3.5 13 13 13
b4 (1+) 3 3.6 1.6 3.1 3.8 2.6 4.3 5.2 3.1 13 13 13
sum 2 1 0.9 1.4 1.2 1.6 2.4 1.6 1.8 13 13 13


Additional Resources and Comments


Assay Details for non-CPTAC-5340 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT3
Peptide Sequence
TDGSFIGYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
39
CPTAC ID
non-CPTAC-5340
Peptide Molecular Mass
986.4709
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.1 3 1.4 8.7 3.2 2.8 12.6 4.4 3.1 15 15 15
y4 (1+) 14.1 3.3 3.4 16.8 5 3.4 21.9 6 4.8 15 15 15
y5 (1+) 7.8 4.6 2.8 5.9 5.2 2.3 9.8 6.9 3.6 15 15 15
y6 (1+) 13 5.2 3.6 12 6.8 4.6 17.7 8.6 5.8 15 15 15
y7 (1+) 5.4 2.7 2.2 7.3 2.8 3.6 9.1 3.9 4.2 15 15 15
sum 3.6 2.1 1.5 5 2.6 2.4 6.2 3.3 2.8 15 15 15


Additional Resources and Comments