Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Fgfr1

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fgfr1 Flg
Sequence Length (AA) Molecular Weight (Da)
822 91981
Protein Name
Fibroblast growth factor receptor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWGWKCLLFW AVLVTATLCT ARPAPTLPEQ AQPWGVPVEV ESLLVHPGDL
60 70 80 90 100
LQLRCRLRDD VQSINWLRDG VQLVESNRTR ITGEEVEVRD SIPADSGLYA
110 120 130 140 150
CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE TDNTKPNRRP
160 170 180 190 200
VAPYWTSPEK MEKKLHAVPA AKTVKFKCPS SGTPNPTLRW LKNGKEFKPD
210 220 230 240 250
HRIGGYKVRY ATWSIIMDSV VPSDKGNYTC IVENEYGSIN HTYQLDVVER
260 270 280 290 300
SPHRPILQAG LPANKTVALG SNVEFMCKVY SDPQPHIQWL KHIEVNGSKI
310 320 330 340 350
GPDNLPYVQI LKTAGVNTTD KEMEVLHLRN VSFEDAGEYT CLAGNSIGLS
360 370 380 390 400
HHSAWLTVLE ALEERPAVMT SPLYLEIIIY CTGAFLISCM LGSVIIYKMK
410 420 430 440 450
SGTKKSDFHS QMAVHKLAKS IPLRRQVTVS ADSSASMNSG VLLVRPSRLS
460 470 480 490 500
SSGTPMLAGV SEYELPEDPR WELPRDRLVL GKPLGEGCFG QVVLAEAIGL
510 520 530 540 550
DKDKPNRVTK VAVKMLKSDA TEKDLSDLIS EMEMMKMIGK HKNIINLLGA
560 570 580 590 600
CTQDGPLYVI VEYASKGNLR EYLQARRPPG LEYCYNPSHN PEEQLSSKDL
610 620 630 640 650
VSCAYQVARG MEYLASKKCI HRDLAARNVL VTEDNVMKIA DFGLARDIHH
660 670 680 690 700
IDYYKKTTNG RLPVKWMAPE ALFDRIYTHQ SDVWSFGVLL WEIFTLGGSP
710 720 730 740 750
YPGVPVEELF KLLKEGHRMD KPSNCTNELY MMMRDCWHAV PSQRPTFKQL
760 770 780 790 800
VEDLDRIVAL TSNQEYLDLS IPLDQYSPSF PDTRSSTCSS GEDSVFSHEP
810 820 822
LPEEPCLPRH PTQLANSGLK RR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1773 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGFR1
Peptide Modified Sequence
DIHHIDY[+80.0]YK
Modification Type
Phospho (Y)
Protein - Site of Modification
653
Peptide - Site of Modification
7
Peptide Start
647
Peptide End
655
CPTAC ID
CPTAC-1773
Peptide Molecular Mass
1,282.5384
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 10.8 10.2 6.9 9.2 8.6 11.3 14.2 13.3 13.2 15 15 15
b3 (1+) 8.8 6.9 7.6 10 6.5 12.8 13.3 9.5 14.9 15 15 15
y3 (1+) 10.8 6.2 9 13.1 6.3 12.2 17 8.8 15.2 15 15 15
y4 (1+) 9.6 6.8 8.5 10.4 7.2 14.4 14.2 9.9 16.7 15 15 15
y5 (1+) 9.1 7.2 9.7 9.2 6.3 14 12.9 9.6 17 15 15 15
y6 (1+) 8.5 5.8 6.5 10.3 5.7 13 13.4 8.1 14.5 15 15 15
sum 7.7 6.2 7.1 9.7 5.6 12.3 12.4 8.4 14.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1780 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGFR1
Peptide Modified Sequence
DLVSC[+57.0]AY[+80.0]QVAR
Modification Type
Carbamidomethyl (C), Phospho (Y)
Protein - Site of Modification
603, 605
Peptide - Site of Modification
5, 7
Peptide Start
599
Peptide End
609
CPTAC ID
CPTAC-1780
Peptide Molecular Mass
1,360.5847
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 80 21.8 5.3 85.2 26.2 5.4 116.9 34.1 7.6 14 15 15
y8 (1+) 22.8 4.7 2.5 27.6 6.7 2.4 35.8 8.2 3.5 15 15 15
y7 (1+) 35.5 11 4.2 50.5 21.1 5 61.7 23.8 6.5 15 15 15
y6 (1+) 33.1 7.6 1.6 44.5 13.3 3.6 55.5 15.3 3.9 15 15 15
y5 (1+) 54.4 12.1 5 55 21.2 4.3 77.4 24.4 6.6 14 15 15
b2 (1+) 23.5 3.4 3 46.3 16.6 4.5 51.9 16.9 5.4 15 15 15
b3 (1+) 86.8 26.9 5.1 102.5 49.5 7.4 134.3 56.3 9 14 15 15
sum 25.7 3.9 1.7 37.7 12.8 1.9 45.6 13.4 2.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-4034 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fgfr1
Peptide Sequence
HPTQLANSGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
810
Peptide End
820
CPTAC ID
non-CPTAC-4034
Peptide Molecular Mass
1,164.6251
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 8.9 5.5 5.6 11.1 13.4 9.3 14.2 14.5 10.9 15 15 15
b4 (1+) 7.6 5.4 4.9 8 6.8 6.4 11 8.7 8.1 15 15 15
b5 (1+) 16.8 13.8 6.7 19.1 30.8 9.3 25.4 33.8 11.5 15 15 15
y5 (1+) 7.8 3.5 6.1 8.5 7.7 9 11.5 8.5 10.9 15 15 15
y6 (1+) 11.4 7.4 3.6 13.6 8.8 6.9 17.7 11.5 7.8 15 15 15
sum 6.2 4.4 2.3 5.4 8 4.2 8.2 9.1 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2818 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGFR1
Peptide Sequence
HPAQLANGGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
810
Peptide End
820
CPTAC ID
CPTAC-2818
Peptide Molecular Mass
1,104.6040
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4 3.6 4.8 6.3 4.7 5 7.5 5.9 6.9 15 15 15
y6 (1+) 5.2 3.2 4.7 6.3 3.4 4.5 8.2 4.7 6.5 15 15 15
y5 (1+) 11 6.2 4.7 17 6.2 4.1 20.2 8.8 6.2 15 15 15
sum 3.4 3.1 2.8 5.6 3.4 2.2 6.6 4.6 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments