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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SCYL3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SCYL3 PACE1
Sequence Length (AA) Molecular Weight (Da)
742 82857
Protein Name
Protein-associating with the carboxyl-terminal domain of ezrin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV
60 70 80 90 100
NKAAKHLKTL RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV
110 120 130 140 150
CAGIYDILLA LIFLHDRGHL THNNVCLSSV FVSEDGHWKL GGMETVCKVS
160 170 180 190 200
QATPEFLRSI QSIRDPASIP PEEMSPEFTT LPECHGHARD AFSFGTLVES
210 220 230 240 250
LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL SHDFFRNDFL
260 270 280 290 300
EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF
310 320 330 340 350
AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE
360 370 380 390 400
EHVRMVLLSH IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL
410 420 430 440 450
AVLVSLLGPE VVVGGERTKI FKRTAPSFTK NTDLSLEDSP MCVVCSHHSQ
460 470 480 490 500
ISPILENPFS SIFPKCFFSG STPINSKKHI QRDYYNTLLQ TGDPFSQPIK
510 520 530 540 550
FPINGLSDVK NTSEDSENFP SSSKKSEEWP DWSEPEEPEN QTVNIQIWPR
560 570 580 590 600
EPCDDVKSQC TTLDVEESSW DDCEPSSLDT KVNPGGGITA TKPVTSGEQK
610 620 630 640 650
PIPALLSLTE ESMPWKSSLP QKISLVQRGD DADQIEPPKV SSQERPLKVP
660 670 680 690 700
SELGLGEEFT IQVKKKPVKD PEMDWFADMI PEIKPSAAFL ILPELRTEMV
710 720 730 740 742
PKKDDVSPVM QFSSKFAAAE ITEGEAEGWE EEGELNWEDN NW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5625 Collapse assay details

Data source: Panorama

Official Gene Symbol
SCYL3
Peptide Sequence
LVPLLLNQLVFAEPVAVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
290
Peptide End
307
CPTAC ID
non-CPTAC-5625
Peptide Molecular Mass
1,962.1918
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 6.9 6 8.6 15.6 6.4 9.8 17.1 8.8 13 15 15 15
y5 (1+) 5.3 6.5 9.7 13.3 6.6 11.4 14.3 9.3 15 15 15 15
y8 (1+) 6.8 5.9 8.6 15.4 6.4 11 16.8 8.7 14 15 15 15
sum 5.6 6.2 9.1 14.2 6.5 10.9 15.3 9 14.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments