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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CSNK1G3 N/A
Sequence Length (AA) Molecular Weight (Da)
447 51389
Protein Name
Casein kinase I isoform gamma-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG
60 70 80 90 100
CGNFGELRLG KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY KQLGSGDGIP
110 120 130 140 150
QVYYFGPCGK YNAMVLELLG PSLEDLFDLC DRTFSLKTVL MIAIQLISRM
160 170 180 190 200
EYVHSKNLIY RDVKPENFLI GRPGNKTQQV IHIIDFGLAK EYIDPETKKH
210 220 230 240 250
IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM YFLRGSLPWQ
260 270 280 290 300
GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD
310 320 330 340 350
YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH
360 370 380 390 400
QHRDKMQQSK NQSADHRAAW DSQQANPHHL RAHLAADRHG GSVQVVSSTN
410 420 430 440 447
GELNTDDPTA GRSNAPITAP TEVEVMDETK CCCFFKRRKR KTIQRHK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5627 Collapse assay details

Data source: Panorama

Official Gene Symbol
CSNK1G3
Peptide Sequence
GTGSSSSGVLMVGPNFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
28
Peptide End
44
CPTAC ID
non-CPTAC-5627
Peptide Molecular Mass
1,651.7988
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 2.3 2.6 6.4 3 2.6 5.7 3.8 3.7 8.6 15 15 15
y5 (1+) 2.4 3 3.3 2.4 3.1 3.6 3.4 4.3 4.9 15 15 15
y8 (1+) 1.9 2.1 4.7 2.5 2.6 6.2 3.1 3.3 7.8 15 15 15
sum 1.9 1.9 2.4 2.2 2.2 3.6 2.9 2.9 4.3 15 15 15


Additional Resources and Comments