STK32B, CPTAC-5633 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK32B	YANK2

Sequence Length (AA)	Molecular Weight (Da)
414	47883

Protein Name
Serine/threonine-protein kinase 32B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGGNHSHKPP	VFDENEEVNF	DHFQILRAIG	KGSFGKVCIV	QKRDTKKMYA
60	70	80	90	100
MKYMNKQKCI	ERDEVRNVFR	ELQIMQGLEH	PFLVNLWYSF	QDEEDMFMVV
110	120	130	140	150
DLLLGGDLRY	HLQQNVHFTE	GTVKLYICEL	ALALEYLQRY	HIIHRDIKPD
160	170	180	190	200
NILLDEHGHV	HITDFNIATV	VKGAERASSM	AGTKPYMAPE	VFQVYMDRGP
210	220	230	240	250
GYSYPVDWWS	LGITAYELLR	GWRPYEIHSV	TPIDEILNMF	KVERVHYSST
260	270	280	290	300
WCKGMVALLR	KLLTKDPESR	VSSLHDIQSV	PYLADMNWDA	VFKKALMPGF
310	320	330	340	350
VPNKGRLNCD	PTFELEEMIL	ESKPLHKKKK	RLAKNRSRDG	TKDSCPLNGH
360	370	380	390	400
LQHCLETVRE	EFIIFNREKL	RRQQGQGSQL	LDTDSRGGGQ	AQSKLQDGCN
410	414
NNLLTHTCTR	GCSS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5633 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK32B
Peptide Sequence: EEFIIFNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 360
Peptide End: 367

CPTAC ID: non-CPTAC-5633
Peptide Molecular Mass: 1,066.5447
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	2.4	2	2.8	3.1	2.8	4.5	3.9	3.4	5.3	15	15	15
y4 (1+)	1.7	1.2	3.7	2.3	2.5	5	2.9	2.8	6.2	15	15	15
y5 (1+)	2.2	1.7	3.8	2.2	2.1	5.6	3.1	2.7	6.8	15	15	15
sum	1.8	1	2.5	2.3	2.2	4.5	2.9	2.4	5.1	15	15	15

STK32B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5633 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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STK32B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5633 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments