DYRK2, CPTAC-5646 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DYRK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
601	66652

Protein Name
Dual specificity tyrosine-phosphorylation-regulated kinase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLTRKPSAAA	PAAYPTGRGG	DSAVRQLQAS	PGLGAGATRS	GVGTGPPSPI
60	70	80	90	100
ALPPLRASNA	AAAAHTIGGS	KHTMNDHLHV	GSHAHGQIQV	QQLFEDNSNK
110	120	130	140	150
RTVLTTQPNG	LTTVGKTGLP	VVPERQLDSI	HRRQGSSTSL	KSMEGMGKVK
160	170	180	190	200
ATPMTPEQAM	KQYMQKLTAF	EHHEIFSYPE	IYFLGLNAKK	RQGMTGGPNN
210	220	230	240	250
GGYDDDQGSY	VQVPHDHVAY	RYEVLKVIGK	GSFGQVVKAY	DHKVHQHVAL
260	270	280	290	300
KMVRNEKRFH	RQAAEEIRIL	EHLRKQDKDN	TMNVIHMLEN	FTFRNHICMT
310	320	330	340	350
FELLSMNLYE	LIKKNKFQGF	SLPLVRKFAH	SILQCLDALH	KNRIIHCDLK
360	370	380	390	400
PENILLKQQG	RSGIKVIDFG	SSCYEHQRVY	TYIQSRFYRA	PEVILGARYG
410	420	430	440	450
MPIDMWSLGC	ILAELLTGYP	LLPGEDEGDQ	LACMIELLGM	PSQKLLDASK
460	470	480	490	500
RAKNFVSSKG	YPRYCTVTTL	SDGSVVLNGG	RSRRGKLRGP	PESREWGNAL
510	520	530	540	550
KGCDDPLFLD	FLKQCLEWDP	AVRMTPGQAL	RHPWLRRRLP	KPPTGEKTSV
560	570	580	590	600
KRITESTGAI	TSISKLPPPS	SSASKLRTNL	AQMTDANGNI	QQRTVLPKLV
601
S

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5646 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK2
Peptide Sequence: APEVILGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 390
Peptide End: 398

CPTAC ID: non-CPTAC-5646
Peptide Molecular Mass: 924.5393
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	1	1.5	2.3	1.8	2.3	2.8	2.1	2.7	3.6	15	15	15
y4 (1+)	1.3	1.7	1.8	2.2	2.8	2.6	2.6	3.3	3.2	15	15	15
y5 (1+)	1.3	1.7	2.5	1.9	1.6	2.9	2.3	2.3	3.8	15	15	15
sum	0.8	0.9	1.7	1.2	1.3	2.1	1.4	1.6	2.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5645 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK2
Peptide Sequence: ITESTGAITSISK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 553
Peptide End: 565

CPTAC ID: non-CPTAC-5645
Peptide Molecular Mass: 1,306.6980
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend