NEK11, CPTAC-5649 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NEK11	N/A

Sequence Length (AA)	Molecular Weight (Da)
645	74192

Protein Name
Serine/threonine-protein kinase Nek11

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLKFQEAAKC	VSGSTAISTY	PKTLIARRYV	LQQKLGSGSF	GTVYLVSDKK
60	70	80	90	100
AKRGEELKVL	KEISVGELNP	NETVQANLEA	QLLSKLDHPA	IVKFHASFVE
110	120	130	140	150
QDNFCIITEY	CEGRDLDDKI	QEYKQAGKIF	PENQIIEWFI	QLLLGVDYMH
160	170	180	190	200
ERRILHRDLK	SKNVFLKNNL	LKIGDFGVSR	LLMGSCDLAT	TLTGTPHYMS
210	220	230	240	250
PEALKHQGYD	TKSDIWSLAC	ILYEMCCMNH	AFAGSNFLSI	VLKIVEGDTP
260	270	280	290	300
SLPERYPKEL	NAIMESMLNK	NPSLRPSAIE	ILKIPYLDEQ	LQNLMCRYSE
310	320	330	340	350
MTLEDKNLDC	QKEAAHIINA	MQKRIHLQTL	RALSEVQKMT	PRERMRLRKL
360	370	380	390	400
QAADEKARKL	KKIVEEKYEE	NSKRMQELRS	RNFQQLSVDV	LHEKTHLKGM
410	420	430	440	450
EEKEEQPEGR	LSCSPQDEDE	ERWQGREEES	DEPTLENLPE	SQPIPSMDLH
460	470	480	490	500
ELESIVEDAT	SDLGYHEIPE	DPLVAEEYYA	DAFDSYCEES	DEEEEEIALE
510	520	530	540	550
RPEKEIRNEG	SQPAYRTNQQ	DSDIEALARC	LENVLGCTSL	DTKTITTMAE
560	570	580	590	600
DMSPGPPIFN	SVMARTKMKR	MRESAMQKLG	TEVFEEVYNY	LKRARHQNAS
610	620	630	640	645
EAEIRECLEK	VVPQASDCFE	VDQLLYFEEQ	LLITMGKEPT	LQNHL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5648 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK11
Peptide Sequence: IVEGDTPSLPER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 244
Peptide End: 255

CPTAC ID: non-CPTAC-5648
Peptide Molecular Mass: 1,311.6670
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	1.5	1.4	2.5	1.9	1.9	4.7	2.4	2.4	5.3	15	15	15
y7 (1+)	1.7	1.3	2.1	1.8	2.4	4.3	2.5	2.7	4.8	15	15	15
y6 (1+)	1.2	1	1.4	3.5	2.8	3.4	3.7	3	3.7	15	15	15
sum	1.3	0.9	1.3	1.6	1.3	3.6	2.1	1.6	3.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5649 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK11
Peptide Sequence: LGSGSFGTVYLVSDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 35
Peptide End: 49

CPTAC ID: non-CPTAC-5649
Peptide Molecular Mass: 1,528.7773
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend