CSNK2A2, CPTAC-5669 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CSNK2A2	CK2A2

Sequence Length (AA)	Molecular Weight (Da)
350	41213

Protein Name
Casein kinase II subunit alpha'

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPGPAAGSRA	RVYAEVNSLR	SREYWDYEAH	VPSWGNQDDY	QLVRKLGRGK
60	70	80	90	100
YSEVFEAINI	TNNERVVVKI	LKPVKKKKIK	REVKILENLR	GGTNIIKLID
110	120	130	140	150
TVKDPVSKTP	ALVFEYINNT	DFKQLYQILT	DFDIRFYMYE	LLKALDYCHS
160	170	180	190	200
KGIMHRDVKP	HNVMIDHQQK	KLRLIDWGLA	EFYHPAQEYN	VRVASRYFKG
210	220	230	240	250
PELLVDYQMY	DYSLDMWSLG	CMLASMIFRR	EPFFHGQDNY	DQLVRIAKVL
260	270	280	290	300
GTEELYGYLK	KYHIDLDPHF	NDILGQHSRK	RWENFIHSEN	RHLVSPEALD
310	320	330	340	350
LLDKLLRYDH	QQRLTAKEAM	EHPYFYPVVK	EQSQPCADNA	VLSSGLTAAR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5669 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK2A2
Peptide Sequence: VLGTEELYGYLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 249
Peptide End: 260

CPTAC ID: non-CPTAC-5669
Peptide Molecular Mass: 1,383.7286
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	6.3	4.9	4.5	9	7.9	5.5	11	9.3	7.1	15	15	15
y10 (1+)	5.3	4	3.3	7.6	5.3	5.2	9.3	6.6	6.2	15	15	15
y4 (1+)	6	4.7	4.8	9	7.7	5.6	10.8	9	7.4	15	15	15
sum	5.4	4.3	2.9	8.1	6.6	4.4	9.7	7.9	5.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5670 Collapse assay details

Data source: Panorama

Official Gene Symbol: CSNK2A2
Peptide Sequence: VYAEVNSLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 12
Peptide End: 20

CPTAC ID: non-CPTAC-5670
Peptide Molecular Mass: 1,049.5506
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend