PIK3C2B, CPTAC-5672 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PIK3C2B	N/A

Sequence Length (AA)	Molecular Weight (Da)
1634	184768

Protein Name
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSTQGNGEH	WKSLESVGIS	RKELAMAEAL	QMEYDALSRL	RHDKEENRAK
60	70	80	90	100
QNADPSLISW	DEPGVDFYSK	PAGRRTDLKL	LRGLSGSDPT	LNYNSLSPQE
110	120	130	140	150
GPPNHSTSQG	PQPGSDPWPK	GSLSGDYLYI	FDGSDGGVSS	SPGPGDIEGS
160	170	180	190	200
CKKLSPPPLP	PRASIWDTPP	LPPRKGSPSS	SKISQPSDIN	TFSLVEQLPG
210	220	230	240	250
KLLEHRILEE	EEVLGGGGQG	RLLGSVDYDG	INDAITRLNL	KSTYDAEMLR
260	270	280	290	300
DATRGWKEGR	GPLDFSKDTS	GKPVARSKTM	PPQVPPRTYA	SRYGNRKNAT
310	320	330	340	350
PGKNRRISAA	PVGSRPHTVA	NGHELFEVSE	ERDEEVAAFC	HMLDILRSGS
360	370	380	390	400
DIQDYFLTGY	VWSAVTPSPE	HLGDEVNLKV	TVLCDRLQEA	LTFTCNCSST
410	420	430	440	450
VDLLIYQTLC	YTHDDLRNVD	VGDFVLKPCG	LEEFLQNKHA	LGSHEYIQYC
460	470	480	490	500
RKFDIDIRLQ	LMEQKVVRSD	LARTVNDDQS	PSTLNYLVHL	QERPVKQTIS
510	520	530	540	550
RQALSLLFDT	YHNEVDAFLL	ADGDFPLKAD	RVVQSVKAIC	NALAAVETPE
560	570	580	590	600
ITSALNQLPP	CPSRMQPKIQ	KDPSVLAVRE	NREKVVEALT	AAILDLVELY
610	620	630	640	650
CNTFNADFQT	AVPGSRKHDL	VQEACHFARS	LAFTVYATHR	IPIIWATSYE
660	670	680	690	700
DFYLSCSLSH	GGKELCSPLQ	TRRAHFSKYL	FHLIVWDQQI	CFPVQVNRLP
710	720	730	740	750
RETLLCATLY	ALPIPPPGSS	SEANKQRRVP	EALGWVTTPL	FNFRQVLTCG
760	770	780	790	800
RKLLGLWPAT	QENPSARWSA	PNFHQPDSVI	LQIDFPTSAF	DIKFTSPPGD
810	820	830	840	850
KFSPRYEFGS	LREEDQRKLK	DIMQKESLYW	LTDADKKRLW	EKRYYCHSEV
860	870	880	890	900
SSLPLVLASA	PSWEWACLPD	IYVLLKQWTH	MNHQDALGLL	HATFPDQEVR
910	920	930	940	950
RMAVQWIGSL	SDAELLDYLP	QLVQALKYEC	YLDSPLVRFL	LKRAVSDLRV
960	970	980	990	1000
THYFFWLLKD	GLKDSQFSIR	YQYLLAALLC	CCGKGLREEF	NRQCWLVNAL
1010	1020	1030	1040	1050
AKLAQQVREA	APSARQGILR	TGLEEVKQFF	ALNGSCRLPL	SPSLLVKGIV
1060	1070	1080	1090	1100
PRDCSYFNSN	AVPLKLSFQN	VDPLGENIRV	IFKCGDDLRQ	DMLTLQMIRI
1110	1120	1130	1140	1150
MSKIWVQEGL	DMRMVIFRCF	STGRGRGMVE	MIPNAETLRK	IQVEHGVTGS
1160	1170	1180	1190	1200
FKDRPLADWL	QKHNPGEDEY	EKAVENFIYS	CAGCCVATYV	LGICDRHNDN
1210	1220	1230	1240	1250
IMLKTTGHMF	HIDFGRFLGH	AQMFGNIKRD	RAPFVFTSDM	AYVINGGDKP
1260	1270	1280	1290	1300
SSRFHDFVDL	CCQAYNLIRK	HTHLFLNLLG	LMLSCGIPEL	SDLEDLKYVY
1310	1320	1330	1340	1350
DALRPQDTEA	NATTYFTRLI	ESSLGSVATK	LNFFIHNLAQ	MKFTGSDDRL
1360	1370	1380	1390	1400
TLSFASRTHT	LKSSGRISDV	FLCRHEKIFH	PNKGYIYVVK	VMRENTHEAT
1410	1420	1430	1440	1450
YIQRTFEEFQ	ELHNKLRLLF	PSSHLPSFPS	RFVIGRSRGE	AVAERRREEL
1460	1470	1480	1490	1500
NGYIWHLIHA	PPEVAECDLV	YTFFHPLPRD	EKAMGTSPAP	KSSDGTWARP
1510	1520	1530	1540	1550
VGKVGGEVKL	SISYKNNKLF	IMVMHIRGLQ	LLQDGNDPDP	YVKIYLLPDP
1560	1570	1580	1590	1600
QKTTKRKTKV	ARKTCNPTYN	EMLVYDGIPK	GDLQQRELQL	SVLSEQGFWE
1610	1620	1630	1634
NVLLGEVNIR	LRELDLAQEK	TGWFALGSRS	HGTL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5672 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3C2B
Peptide Modified Sequence: ISDVFLC[+57.021464]R
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1367
Peptide End: 1374

CPTAC ID: non-CPTAC-5672
Peptide Molecular Mass: 1,008.5063
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	2	5.6	6.2	1.8	5.8	10.2	2.7	8.1	11.9	15	15	15
y5 (1+)	2.8	3.7	6.2	3.3	4.1	7.5	4.3	5.5	9.7	15	15	15
y4 (1+)	3	5.3	5.6	5.2	5.7	8.8	6	7.8	10.4	15	15	15
sum	2.3	4.3	2.8	2.7	4.5	7.5	3.5	6.2	8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5673 Collapse assay details

Data source: Panorama

Official Gene Symbol: PIK3C2B
Peptide Sequence: LIESSLGSVATK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1319
Peptide End: 1330

CPTAC ID: non-CPTAC-5673
Peptide Molecular Mass: 1,203.6711
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend