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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
NRBP2 N/A
Sequence Length (AA) Molecular Weight (Da)
501 57803
Protein Name
Nuclear receptor-binding protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPEPAPRR ARERERERED ESEDESDILE ESPCGRWQKR REQVNQGNMP
60 70 80 90 100
GLQSTFLAMD TEEGVEVVWN ELHFGDRKAF AAHEEKIQTV FEQLVLVDHP
110 120 130 140 150
NIVKLHKYWL DTSEACARVI FITEYVSSGS LKQFLKKTKK NHKAMNARAW
160 170 180 190 200
KRWCTQILSA LSFLHACSPP IIHGNLTSDT IFIQHNGLIK IGSVWHRIFS
210 220 230 240 250
NALPDDLRSP IRAEREELRN LHFFPPEYGE VADGTAVDIF SFGMCALEMA
260 270 280 290 300
VLEIQTNGDT RVTEEAIARA RHSLSDPNMR EFILCCLARD PARRPSAHSL
310 320 330 340 350
LFHRVLFEVH SLKLLAAHCF IQHQYLMPEN VVEEKTKAMD LHAVLAELPR
360 370 380 390 400
PRRPPLQWRY SEVSFMELDK FLEDVRNGIY PLMNFAATRP LGLPRVLAPP
410 420 430 440 450
PEEVQKAKTP TPEPFDSETR KVIQMQCNLE RSEDKARWHL TLLLVLEDRL
460 470 480 490 500
HRQLTYDLLP TDSAQDLASE LVHYGFLHED DRMKLAAFLE STFLKYRGTQ
501
A

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5674 Collapse assay details

Data source: Panorama

Official Gene Symbol
NRBP2
Peptide Sequence
LAAFLESTFLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
485
Peptide End
495
CPTAC ID
non-CPTAC-5674
Peptide Molecular Mass
1,238.6911
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13 8.9 5.8 14.4 9.9 8.5 19.4 13.3 10.3 15 15 15
y5 (1+) 13.5 8.8 6.2 14.4 9.6 9.6 19.7 13 11.4 15 15 15
y7 (1+) 12.4 8.6 5.2 13.5 8.8 7.6 18.3 12.3 9.2 15 15 15
sum 12.8 8.7 5.4 13.9 9.3 8.2 18.9 12.7 9.8 15 15 15


Additional Resources and Comments