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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CDC42BPA

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDC42BPA KIAA0451
Sequence Length (AA) Molecular Weight (Da)
1732 197307
Protein Name
Serine/threonine-protein kinase MRCK alpha
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE
60 70 80 90 100
KNILEYLEWA KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD
110 120 130 140 150
KVFAMKILNK WEMLKRAETA CFREERDVLV NGDNKWITTL HYAFQDDNNL
160 170 180 190 200
YLVMDYYVGG DLLTLLSKFE DRLPEDMARF YLAEMVIAID SVHQLHYVHR
210 220 230 240 250
DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT PDYISPEILQ
260 270 280 290 300
AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER
310 320 330 340 350
FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN
360 370 380 390 400
IRNCEAPYIP EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV
410 420 430 440 450
GFTYTSSCVL SDRSCLRVTA GPTSLDLDVN VQRTLDNNLA TEAYERRIKR
460 470 480 490 500
LEQEKLELSR KLQESTQTVQ ALQYSTVDGP LTASKDLEIK NLKEEIEKLR
510 520 530 540 550
KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL QQEREDLNKE
560 570 580 590 600
LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV
610 620 630 640 650
RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE
660 670 680 690 700
QSEHYSKQLE NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY
710 720 730 740 750
EEELSKREGI HANEIKNLKK ELHDSEGQQL ALNKEIMILK DKLEKTRRES
760 770 780 790 800
QSEREEFESE FKQQYEREKV LLTEENKKLT SELDKLTTLY ENLSIHNQQL
810 820 830 840 850
EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA LASKMTEELE
860 870 880 890 900
ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL
910 920 930 940 950
NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ
960 970 980 990 1000
HSFLAFLNTP TDALDQFERS PSCTPASKGR RTVDSTPLSV HTPTLRKKGC
1010 1020 1030 1040 1050
PGSTGFPPKR KTHQFFVKSF TTPTKCHQCT SLMVGLIRQG CSCEVCGFSC
1060 1070 1080 1090 1100
HITCVNKAPT TCPVPPEQTK GPLGIDPQKG IGTAYEGHVR IPKPAGVKKG
1110 1120 1130 1140 1150
WQRALAIVCD FKLFLYDIAE GKASQPSVVI SQVIDMRDEE FSVSSVLASD
1160 1170 1180 1190 1200
VIHASRKDIP CIFRVTASQL SASNNKCSIL MLADTENEKN KWVGVLSELH
1210 1220 1230 1240 1250
KILKKNKFRD RSVYVPKEAY DSTLPLIKTT QAAAIIDHER IALGNEEGLF
1260 1270 1280 1290 1300
VVHVTKDEII RVGDNKKIHQ IELIPNDQLV AVISGRNRHV RLFPMSALDG
1310 1320 1330 1340 1350
RETDFYKLSE TKGCQTVTSG KVRHGALTCL CVAMKRQVLC YELFQSKTRH
1360 1370 1380 1390 1400
RKFKEIQVPY NVQWMAIFSE QLCVGFQSGF LRYPLNGEGN PYSMLHSNDH
1410 1420 1430 1440 1450
TLSFIAHQPM DAICAVEISS KEYLLCFNSI GIYTDCQGRR SRQQELMWPA
1460 1470 1480 1490 1500
NPSSCCYNAP YLSVYSENAV DIFDVNSMEW IQTLPLKKVR PLNNEGSLNL
1510 1520 1530 1540 1550
LGLETIRLIY FKNKMAEGDE LVVPETSDNS RKQMVRNINN KRRYSFRVPE
1560 1570 1580 1590 1600
EERMQQRREM LRDPEMRNKL ISNPTNFNHI AHMGPGDGIQ ILKDLPMNPR
1610 1620 1630 1640 1650
PQESRTVFSG SVSIPSITKS RPEPGRSMSA SSGLSARSSA QNGSALKREF
1660 1670 1680 1690 1700
SGGSYSAKRQ PMPSPSEGSL SSGGMDQGSD APARDFDGED SDSPRHSTAS
1710 1720 1730 1732
NSSNLSSPPS PASPRKTKSL SLESTDRGSW DP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5684 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC42BPA
Peptide Sequence
LFLYDIAEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1113
Peptide End
1122
CPTAC ID
non-CPTAC-5684
Peptide Molecular Mass
1,167.6176
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 3.7 3.2 3.7 3.3 3.6 4.1 5 4.8 5.5 15 15 15
y4 (1+) 3.8 4.7 6.8 5.5 6.6 9.5 6.7 8.1 11.7 15 15 15
y7 (1+) 3.6 4.1 6.8 4.7 4.6 8.2 5.9 6.2 10.7 15 15 15
sum 3.6 3.8 4.3 4.2 4 5.6 5.5 5.5 7.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments