DYRK3, CPTAC-5689 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DYRK3	N/A

Sequence Length (AA)	Molecular Weight (Da)
588	65714

Protein Name
Dual specificity tyrosine-phosphorylation-regulated kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGGTARGPGR	KDAGPPGAGL	PPQQRRLGDG	VYDTFMMIDE	TKCPPCSNVL
60	70	80	90	100
CNPSEPPPPR	RLNMTTEQFT	GDHTQHFLDG	GEMKVEQLFQ	EFGNRKSNTI
110	120	130	140	150
QSDGISDSEK	CSPTVSQGKS	SDCLNTVKSN	SSSKAPKVVP	LTPEQALKQY
160	170	180	190	200
KHHLTAYEKL	EIINYPEIYF	VGPNAKKRHG	VIGGPNNGGY	DDADGAYIHV
210	220	230	240	250
PRDHLAYRYE	VLKIIGKGSF	GQVARVYDHK	LRQYVALKMV	RNEKRFHRQA
260	270	280	290	300
AEEIRILEHL	KKQDKTGSMN	VIHMLESFTF	RNHVCMAFEL	LSIDLYELIK
310	320	330	340	350
KNKFQGFSVQ	LVRKFAQSIL	QSLDALHKNK	IIHCDLKPEN	ILLKHHGRSS
360	370	380	390	400
TKVIDFGSSC	FEYQKLYTYI	QSRFYRAPEI	ILGSRYSTPI	DIWSFGCILA
410	420	430	440	450
ELLTGQPLFP	GEDEGDQLAC	MMELLGMPPP	KLLEQSKRAK	YFINSKGIPR
460	470	480	490	500
YCSVTTQADG	RVVLVGGRSR	RGKKRGPPGS	KDWGTALKGC	DDYLFIEFLK
510	520	530	540	550
RCLHWDPSAR	LTPAQALRHP	WISKSVPRPL	TTIDKVSGKR	VVNPASAFQG
560	570	580	588
LGSKLPPVVG	IANKLKANLM	SETNGSIPLC	SVLPKLIS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5689 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK3
Peptide Sequence: VVNPASAFQGLGSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 541
Peptide End: 554

CPTAC ID: non-CPTAC-5689
Peptide Molecular Mass: 1,373.7303
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	2.4	2.2	3.9	2.7	2.3	6.5	3.6	3.2	7.6	15	15	15
y9 (1+)	3.5	1.5	6.4	3.5	1.7	9	4.9	2.3	11	15	15	15
y7 (1+)	2.8	1.4	5.8	3.3	2.4	6	4.3	2.8	8.3	15	15	15
sum	2.7	1.3	2.4	2.7	1.7	5.7	3.8	2.1	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5690 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK3
Peptide Sequence: VVPLTPEQALK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 138
Peptide End: 148

CPTAC ID: non-CPTAC-5690
Peptide Molecular Mass: 1,193.7020
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend