CDC42BPG, CPTAC-5992 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDC42BPG	DMPK2

Sequence Length (AA)	Molecular Weight (Da)
1551	172459

Protein Name
Serine/threonine-protein kinase MRCK gamma

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MERRLRALEQ	LARGEAGGCP	GLDGLLDLLL	ALHHELSSGP	LRRERSVAQF
60	70	80	90	100
LSWASPFVSK	VKELRLQRDD	FEILKVIGRG	AFGEVTVVRQ	RDTGQIFAMK
110	120	130	140	150
MLHKWEMLKR	AETACFREER	DVLVKGDSRW	VTTLHYAFQD	EEYLYLVMDY
160	170	180	190	200
YAGGDLLTLL	SRFEDRLPPE	LAQFYLAEMV	LAIHSLHQLG	YVHRDVKPDN
210	220	230	240	250
VLLDVNGHIR	LADFGSCLRL	NTNGMVDSSV	AVGTPDYISP	EILQAMEEGK
260	270	280	290	300
GHYGPQCDWW	SLGVCAYELL	FGETPFYAES	LVETYGKIMN	HEDHLQFPPD
310	320	330	340	350
VPDVPASAQD	LIRQLLCRQE	ERLGRGGLDD	FRNHPFFEGV	DWERLASSTA
360	370	380	390	400
PYIPELRGPM	DTSNFDVDDD	TLNHPGTLPP	PSHGAFSGHH	LPFVGFTYTS
410	420	430	440	450
GSHSPESSSE	AWAALERKLQ	CLEQEKVELS	RKHQEALHAP	TDHRELEQLR
460	470	480	490	500
KEVQTLRDRL	PEMLRDKASL	SQTDGPPAGS	PGQDSDLRQE	LDRLHRELAE
510	520	530	540	550
GRAGLQAQEQ	ELCRAQGQQE	ELLQRLQEAQ	EREAATASQT	RALSSQLEEA
560	570	580	590	600
RAAQRELEAQ	VSSLSRQVTQ	LQGQWEQRLE	ESSQAKTIHT	ASETNGMGPP
610	620	630	640	650
EGGPQEAQLR	KEVAALREQL	EQAHSHRPSG	KEEALCQLQE	ENRRLSREQE
660	670	680	690	700
RLEAELAQEQ	ESKQRLEGER	RETESNWEAQ	LADILSWVND	EKVSRGYLQA
710	720	730	740	750
LATKMAEELE	SLRNVGTQTL	PARPLDHQWK	ARRLQKMEAS	ARLELQSALE
760	770	780	790	800
AEIRAKQGLQ	ERLTQVQEAQ	LQAERRLQEA	EKQSQALQQE	LAMLREELRA
810	820	830	840	850
RGPVDTKPSN	SLIPFLSFRS	SEKDSAKDPG	ISGEATRHGG	EPDLRPEGRR
860	870	880	890	900
SLRMGAVFPR	APTANTASTE	GLPAKPGSHT	LRPRSFPSPT	KCLRCTSLML
910	920	930	940	950
GLGRQGLGCD	ACGYFCHTTC	APQAPPCPVP	PDLLRTALGV	HPETGTGTAY
960	970	980	990	1000
EGFLSVPRPS	GVRRGWQRVF	AALSDSRLLL	FDAPDLRLSP	PSGALLQVLD
1010	1020	1030	1040	1050
LRDPQFSATP	VLASDVIHAQ	SRDLPRIFRV	TTSQLAVPPT	TCTVLLLAES
1060	1070	1080	1090	1100
EGERERWLQV	LGELQRLLLD	ARPRPRPVYT	LKEAYDNGLP	LLPHTLCAAI
1110	1120	1130	1140	1150
LDQDRLALGT	EEGLFVIHLR	SNDIFQVGEC	RRVQQLTLSP	SAGLLVVLCG
1160	1170	1180	1190	1200
RGPSVRLFAL	AELENIEVAG	AKIPESRGCQ	VLAAGSILQA	RTPVLCVAVK
1210	1220	1230	1240	1250
RQVLCYQLGP	GPGPWQRRIR	ELQAPATVQS	LGLLGDRLCV	GAAGGFALYP
1260	1270	1280	1290	1300
LLNEAAPLAL	GAGLVPEELP	PSRGGLGEAL	GAVELSLSEF	LLLFTTAGIY
1310	1320	1330	1340	1350
VDGAGRKSRG	HELLWPAAPM	GWGYAAPYLT	VFSENSIDVF	DVRRAEWVQT
1360	1370	1380	1390	1400
VPLKKVRPLN	PEGSLFLYGT	EKVRLTYLRN	QLAEKDEFDI	PDLTDNSRRQ
1410	1420	1430	1440	1450
LFRTKSKRRF	FFRVSEEQQK	QQRREMLKDP	FVRSKLISPP	TNFNHLVHVG
1460	1470	1480	1490	1500
PANGRPGARD	KSPAPEEKGR	VARGSGPQRP	HSFSEALRRP	ASMGSEGLGG
1510	1520	1530	1540	1550
DADPMKRKPW	TSLSSESVSC	PQGSLSPATS	LMQVSERPRS	LPLSPELESS
1551
P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-5992 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDC42BPG
Peptide Sequence: LLLFDAPDLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 978
Peptide End: 987

CPTAC ID: non-CPTAC-5992
Peptide Molecular Mass: 1,171.6601
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	4	3.8	4.3	4.8	4.1	5.3	6.2	5.6	6.8	15	15	15
y7 (1+)	4.6	3.8	4.7	5.6	5.5	5.5	7.2	6.7	7.2	15	15	15
y6 (1+)	4.4	4.3	4.8	5.5	5.4	5.8	7	6.9	7.5	15	15	15
sum	4.2	3.7	4.4	5.1	4.6	5.4	6.6	5.9	7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5991 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDC42BPG
Peptide Sequence: VFAALSDSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 969
Peptide End: 977

CPTAC ID: non-CPTAC-5991
Peptide Molecular Mass: 964.4978
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend