RIOK3, CPTAC-6001 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIOK3	SUDD

Sequence Length (AA)	Molecular Weight (Da)
519	59093

Protein Name
Serine/threonine-protein kinase RIO3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDLVGVASPE	PGTAAAWGPS	KCPWAIPQNT	ISCSLADVMS	EQLAKELQLE
60	70	80	90	100
EEAAVFPEVA	VAEGPFITGE	NIDTSSDLML	AQMLQMEYDR	EYDAQLRREE
110	120	130	140	150
KKFNGDSKVS	ISFENYRKVH	PYEDSDSSED	EVDWQDTRDD	PYRPAKPVPT
160	170	180	190	200
PKKGFIGKGK	DITTKHDEVV	CGRKNTARME	NFAPEFQVGD	GIGMDLKLSN
210	220	230	240	250
HVFNALKQHA	YSEERRSARL	HEKKEHSTAE	KAVDPKTRLL	MYKMVNSGML
260	270	280	290	300
ETITGCISTG	KESVVFHAYG	GSMEDEKEDS	KVIPTECAIK	VFKTTLNEFK
310	320	330	340	350
NRDKYIKDDF	RFKDRFSKLN	PRKIIRMWAE	KEMHNLARMQ	RAGIPCPTVV
360	370	380	390	400
LLKKHILVMS	FIGHDQVPAP	KLKEVKLNSE	EMKEAYYQTL	HLMRQLYHEC
410	420	430	440	450
TLVHADLSEY	NMLWHAGKVW	LIDVSQSVEP	THPHGLEFLF	RDCRNVSQFF
460	470	480	490	500
QKGGVKEALS	ERELFNAVSG	LNITADNEAD	FLAEIEALEK	MNEDHVQKNG
510	519
RKAASFLKDD	GDPPLLYDE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6001 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIOK3
Peptide Sequence: VSISFENYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 109
Peptide End: 117

CPTAC ID: non-CPTAC-6001
Peptide Molecular Mass: 1,113.5455
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	6.4	3.5	2	8.7	4.6	4.6	10.8	5.8	5	15	15	15
y6 (1+)	5.3	2.7	3	9.5	3.5	5.5	10.9	4.4	6.3	15	15	15
y7 (1+)	6.4	6.4	4.3	10.1	7.1	5.5	12	9.6	7	15	15	15
sum	4	2.8	2.4	6.5	3.2	4.4	7.6	4.3	5	15	15	15

RIOK3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6001 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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RIOK3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6001 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments