STK38L, CPTAC-6003 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK38L	KIAA0965, NDR2

Sequence Length (AA)	Molecular Weight (Da)
464	54003

Protein Name
Serine/threonine-protein kinase 38-like

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAMTAGTTTT	FPMSNHTRER	VTVAKLTLEN	FYSNLILQHE	ERETRQKKLE
60	70	80	90	100
VAMEEEGLAD	EEKKLRRSQH	ARKETEFLRL	KRTRLGLDDF	ESLKVIGRGA
110	120	130	140	150
FGEVRLVQKK	DTGHIYAMKI	LRKSDMLEKE	QVAHIRAERD	ILVEADGAWV
160	170	180	190	200
VKMFYSFQDK	RNLYLIMEFL	PGGDMMTLLM	KKDTLTEEET	QFYISETVLA
210	220	230	240	250
IDAIHQLGFI	HRDIKPDNLL	LDAKGHVKLS	DFGLCTGLKK	AHRTEFYRNL
260	270	280	290	300
THNPPSDFSF	QNMNSKRKAE	TWKKNRRQLA	YSTVGTPDYI	APEVFMQTGY
310	320	330	340	350
NKLCDWWSLG	VIMYEMLIGY	PPFCSETPQE	TYRKVMNWKE	TLVFPPEVPI
360	370	380	390	400
SEKAKDLILR	FCIDSENRIG	NSGVEEIKGH	PFFEGVDWEH	IRERPAAIPI
410	420	430	440	450
EIKSIDDTSN	FDDFPESDIL	QPVPNTTEPD	YKSKDWVFLN	YTYKRFEGLT
460	464
QRGSIPTYMK	AGKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6003 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK38L
Peptide Sequence: DIKPDNLLLDAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 213
Peptide End: 224

CPTAC ID: non-CPTAC-6003
Peptide Molecular Mass: 1,353.7504
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	11.6	4.8	3.4	10.7	5.6	4.7	15.8	7.4	5.8	15	15	15
y4 (1+)	7.4	3.4	4.2	8.8	5.3	6.6	11.5	6.3	7.8	15	15	15
y9 (1+)	4.5	2.5	2.5	9.3	4.4	4.2	10.3	5.1	4.9	15	15	15
sum	3.9	2.3	2.5	7.1	4.4	4.5	8.1	5	5.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6002 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK38L
Peptide Sequence: LGLDDFESLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 85
Peptide End: 94

CPTAC ID: non-CPTAC-6002
Peptide Molecular Mass: 1,135.5761
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend