TESK1, CPTAC-6005 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TESK1	N/A

Sequence Length (AA)	Molecular Weight (Da)
626	67684

Protein Name
Dual specificity testis-specific protein kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGERPPLRG	PGPGPGEVPG	EGPPGPGGTG	GGPGRGRPSS	YRALRSAVSS
60	70	80	90	100
LARVDDFHCA	EKIGAGFFSE	VYKVRHRQSG	QVMVLKMNKL	PSNRGNTLRE
110	120	130	140	150
VQLMNRLRHP	NILRFMGVCV	HQGQLHALTE	YMNGGTLEQL	LSSPEPLSWP
160	170	180	190	200
VRLHLALDIA	RGLRYLHSKG	VFHRDLTSKN	CLVRREDRGF	TAVVGDFGLA
210	220	230	240	250
EKIPVYREGA	RKEPLAVVGS	PYWMAPEVLR	GELYDEKADV	FAFGIVLCEL
260	270	280	290	300
IARVPADPDY	LPRTEDFGLD	VPAFRTLVGD	DCPLPFLLLA	IHCCNLEPST
310	320	330	340	350
RAPFTEITQH	LEWILEQLPE	PAPLTRTALT	HNQGSVARGG	PSATLPRPDP
360	370	380	390	400
RLSRSRSDLF	LPPSPESPPN	WGDNLTRVNP	FSLREDLRGG	KIKLLDTPSK
410	420	430	440	450
PVLPLVPPSP	FPSTQLPLVT	TPETLVQPGT	PARRCRSLPS	SPELPRRMET
460	470	480	490	500
ALPGPGPPAV	GPSAEEKMEC	EGSSPEPEPP	GPAPQLPLAV	ATDNFISTCS
510	520	530	540	550
SASQPWSPRS	GPVLNNNPPA	VVVNSPQGWA	GEPWNRAQHS	LPRAAALERT
560	570	580	590	600
EPSPPPSAPR	EPDEGLPCPG	CCLGPFSFGF	LSMCPRPTPA	VARYRNLNCE
610	620	626
AGSLLCHRGH	HAKPPTPSLQ	LPGARS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-6005 Collapse assay details

Data source: Panorama

Official Gene Symbol: TESK1
Peptide Sequence: TEDFGLDVPAFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 264
Peptide End: 275

CPTAC ID: non-CPTAC-6005
Peptide Molecular Mass: 1,365.6565
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	4.2	2.3	1.8	6.6	3.5	3	7.8	4.2	3.5	15	15	15
y5 (1+)	4.2	1.7	2.1	6.9	3.8	3.6	8.1	4.2	4.2	15	15	15
y4 (1+)	4.6	2	2	6.3	2.5	2.9	7.8	3.2	3.5	15	15	15
sum	4.2	1.9	1.9	6.5	3.2	3	7.7	3.7	3.6	15	15	15

TESK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6005 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TESK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6005 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments