ADCK1, CPTAC-6012 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADCK1	N/A

Sequence Length (AA)	Molecular Weight (Da)
530	60577

Protein Name
AarF domain-containing protein kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MARKALKLAS	WTSMALAASG	IYFYSNKYLD	PNDFGAVRVG	RAVATTAVIS
60	70	80	90	100
YDYLTSLKSV	PYGSEEYLQL	RSKSWPVFLQ	VHLRSARRLC	ELCCANRGTF
110	120	130	140	150
IKVGQHLGAL	DYLLPEEYTS	TLKVLHSQAP	QSSMQEIRQV	IREDLGKEIH
160	170	180	190	200
DLFQSFDDTP	LGTASLAQVH	KAVLHDGRTV	AVKVQHPKVR	AQSSKDILLM
210	220	230	240	250
EVLVLAVKQL	FPEFEFMWLV	DEAKKNLPLE	LDFLNEGRNA	EKVSQMLRHF
260	270	280	290	300
DFLKVPRIHW	DLSTERVLLM	EFVDGGQVND	RDYMERNKID	VNEISRHLGK
310	320	330	340	350
MYSEMIFVNG	FVHCDPHPGN	VLVRKHPGTG	KAEIVLLDHG	LYQMLTEEFR
360	370	380	390	400
LNYCHLWQSL	IWTDMKRVKE	YSQRLGAGDL	YPLFACMLTA	RSWDSVNRGI
410	420	430	440	450
SQAPVTATED	LEIRNNAANY	LPQISHLLNH	VPRQMLLILK	TNDLLRGIEA
460	470	480	490	500
ALGTRASASS	FLNMSRCCIR	ALAEHKKKNT	CSFFRRTQIS	FSEAFNLWQI
510	520	530
NLHELILRVK	GLKLADRVLA	LICWLFPAPL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6013 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADCK1
Peptide Sequence: GIEAALGTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 447
Peptide End: 455

CPTAC ID: non-CPTAC-6013
Peptide Molecular Mass: 886.4872
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	2.5	0.8	0.9	4	3	3	4.7	3.1	3.1	15	15	15
y6 (1+)	2.3	1.9	0.9	2.6	2.8	2.3	3.5	3.4	2.5	15	15	15
y3 (1+)	3.2	1.4	1.1	3.1	1.9	1.8	4.5	2.4	2.1	15	15	15
sum	2.3	0.9	0.8	2.7	1.9	1.9	3.5	2.1	2.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-6012 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADCK1
Peptide Sequence: YLDPNDFGAVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 28
Peptide End: 38

CPTAC ID: non-CPTAC-6012
Peptide Molecular Mass: 1,265.6041
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend