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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

DSTYK

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DSTYK KIAA0472, RIP5, RIPK5, SGK496
Sequence Length (AA) Molecular Weight (Da)
929 105206
Protein Name
Dual serine/threonine and tyrosine protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGDGVPWGS EPVSGPGPGG GGMIRELCRG FGRYRRYLGR LRQNLRETQK
60 70 80 90 100
FFRDIKCSHN HTCLSSLTGG GGAERGPAGD VAETGLQAGQ LSCISFPPKE
110 120 130 140 150
EKYLQQIVDC LPCILILGQD CNVKCQLLNL LLGVQVLPTT KLGSEESCKL
160 170 180 190 200
RRLRFTYGTQ TRVSLALPGQ YELVHTLVAH QGNWETIPEE DLEVQENNED
210 220 230 240 250
AAHVLAELEV TMHHALLQEV DVVVAPCQGL RPTVDVLGDL VNDFLPVITY
260 270 280 290 300
ALHKDELSER DEQELQEIRK YFSFPVFFFK VPKLGSEIID SSTRRMESER
310 320 330 340 350
SPLYRQLIDL GYLSSSHWNC GAPGQDTKAQ SMLVEQSEKL RHLSTFSHQV
360 370 380 390 400
LQTRLVDAAK ALNLVHCHCL DIFINQAFDM QRDLQITPKR LEYTRKKENE
410 420 430 440 450
LYESLMNIAN RKQEEMKDMI VETLNTMKEE LLDDATNMEF KDVIVPENGE
460 470 480 490 500
PVGTREIKCC IRQIQELIIS RLNQAVANKL ISSVDYLRES FVGTLERCLQ
510 520 530 540 550
SLEKSQDVSV HITSNYLKQI LNAAYHVEVT FHSGSSVTRM LWEQIKQIIQ
560 570 580 590 600
RITWVSPPAI TLEWKRKVAQ EAIESLSASK LAKSICSQFR TRLNSSHEAF
610 620 630 640 650
AASLRQLEAG HSGRLEKTED LWLRVRKDHA PRLARLSLES CSLQDVLLHR
660 670 680 690 700
KPKLGQELGR GQYGVVYLCD NWGGHFPCAL KSVVPPDEKH WNDLALEFHY
710 720 730 740 750
MRSLPKHERL VDLHGSVIDY NYGGGSSIAV LLIMERLHRD LYTGLKAGLT
760 770 780 790 800
LETRLQIALD VVEGIRFLHS QGLVHRDIKL KNVLLDKQNR AKITDLGFCK
810 820 830 840 850
PEAMMSGSIV GTPIHMAPEL FTGKYDNSVD VYAFGILFWY ICSGSVKLPE
860 870 880 890 900
AFERCASKDH LWNNVRRGAR PERLPVFDEE CWQLMEACWD GDPLKRPLLG
910 920 929
IVQPMLQGIM NRLCKSNSEQ PNRGLDDST

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6022 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSTYK
Peptide Sequence
DEQELQEIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
261
Peptide End
269
CPTAC ID
non-CPTAC-6022
Peptide Molecular Mass
1,158.5517
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 2.2 1.4 1.9 4.6 2.8 3.4 5.1 3.1 3.9 15 15 15
y6 (1+) 2.7 1.4 1.9 5.2 3.2 2.9 5.9 3.5 3.5 15 15 15
y4 (1+) 3.3 0.9 2 4.5 2.9 3.3 5.6 3 3.9 15 15 15
sum 2.1 1.1 1.8 4.5 2.9 3.1 5 3.1 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6023 Collapse assay details

Data source: Panorama

Official Gene Symbol
DSTYK
Peptide Sequence
TEDLWLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
618
Peptide End
624
CPTAC ID
non-CPTAC-6023
Peptide Molecular Mass
931.4763
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.7 3.7 4.1 5.2 3.9 4.3 7 5.4 5.9 15 15 15
y4 (1+) 6.5 3.7 2.9 16.3 5.2 4.6 17.5 6.4 5.4 15 15 15
y3 (1+) 5.6 5 4.9 8 9.5 6.7 9.8 10.7 8.3 15 15 15
sum 4.1 3.8 3.5 5.9 3.6 4.6 7.2 5.2 5.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments