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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

HIPK3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
HIPK3 DYRK6, FIST3, PKY
Sequence Length (AA) Molecular Weight (Da)
1215 133743
Protein Name
Homeodomain-interacting protein kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASQVLVYPP YVYQTQSSAF CSVKKLKVEP SSCVFQERNY PRTYVNGRNF
60 70 80 90 100
GNSHPPTKGS AFQTKIPFNR PRGHNFSLQT SAVVLKNTAG ATKVIAAQAQ
110 120 130 140 150
QAHVQAPQIG AWRNRLHFLE GPQRCGLKRK SEELDNHSSA MQIVDELSIL
160 170 180 190 200
PAMLQTNMGN PVTVVTATTG SKQNCTTGEG DYQLVQHEVL CSMKNTYEVL
210 220 230 240 250
DFLGRGTFGQ VVKCWKRGTN EIVAIKILKN HPSYARQGQI EVSILARLST
260 270 280 290 300
ENADEYNFVR AYECFQHRNH TCLVFEMLEQ NLYDFLKQNK FSPLPLKVIR
310 320 330 340 350
PILQQVATAL KKLKSLGLIH ADLKPENIML VDPVRQPYRV KVIDFGSASH
360 370 380 390 400
VSKTVCSTYL QSRYYRAPEI ILGLPFCEAI DMWSLGCVIA ELFLGWPLYP
410 420 430 440 450
GALEYDQIRY ISQTQGLPGE QLLNVGTKST RFFCKETDMS HSGWRLKTLE
460 470 480 490 500
EHEAETGMKS KEARKYIFNS LDDVAHVNTV MDLEGSDLLA EKADRREFVS
510 520 530 540 550
LLKKMLLIDA DLRITPAETL NHPFVNMKHL LDFPHSNHVK SCFHIMDICK
560 570 580 590 600
SHLNSCDTNN HNKTSLLRPV ASSSTATLTA NFTKIGTLRS QALTTSAHSV
610 620 630 640 650
VHHGIPLQAG TAQFGCGDAF QQTLIICPPA IQGIPATHGK PTSYSIRVDN
660 670 680 690 700
TVPLVTQAPA VQPLQIRPGV LSQTWSGRTQ QMLVPAWQQV TPLAPATTTL
710 720 730 740 750
TSESVAGSHR LGDWGKMISC SNHYNSVMPQ PLLTNQITLS APQPVSVGIA
760 770 780 790 800
HVVWPQPATT KKNKQCQNRG ILVKLMEWEP GREEINAFSW SNSLQNTNIP
810 820 830 840 850
HSAFISPKII NGKDVEEVSC IETQDNQNSE GEARNCCETS IRQDSDSSVS
860 870 880 890 900
DKQRQTIIIA DSPSPAVSVI TISSDTDEEE TSQRHSLREC KGSLDCEACQ
910 920 930 940 950
STLNIDRMCS LSSPDSTLST SSSGQSSPSP CKRPNSMSDE EQESSCDTVD
960 970 980 990 1000
GSPTSDSSGH DSPFAESTFV EDTHENTELV SSADTETKPA VCSVVVPPVE
1010 1020 1030 1040 1050
LENGLNADEH MANTDSICQP LIKGRSAPGR LNQPSAVGTR QQKLTSAFQQ
1060 1070 1080 1090 1100
QHLNFSQVQH FGSGHQEWNG NFGHRRQQAY IPTSVTSNPF TLSHGSPNHT
1110 1120 1130 1140 1150
AVHAHLAGNT HLGGQPTLLP YPSSATLSSA APVAHLLASP CTSRPMLQHP
1160 1170 1180 1190 1200
TYNISHPSGI VHQVPVGLNP RLLPSPTIHQ TQYKPIFPPH SYIAASPAYT
1210 1215
GFPLSPTKLS QYPYM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6026 Collapse assay details

Data source: Panorama

Official Gene Symbol
HIPK3
Peptide Sequence
NTYEVLDFLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
195
Peptide End
205
CPTAC ID
non-CPTAC-6026
Peptide Molecular Mass
1,325.6616
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.3 3.6 3.3 5.8 4 3.6 8.6 5.4 4.9 15 15 15
y4 (1+) 6.6 4.2 2.7 5.4 4.2 2.6 8.5 5.9 3.7 15 15 15
y7 (1+) 7.3 4.8 2.8 6.6 4.6 3.1 9.8 6.6 4.2 15 15 15
sum 6.6 4.1 2.9 5.7 4.1 3 8.7 5.8 4.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-6027 Collapse assay details

Data source: Panorama

Official Gene Symbol
HIPK3
Peptide Sequence
YISQTQGLPGEQLLNVGTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
410
Peptide End
428
CPTAC ID
non-CPTAC-6027
Peptide Molecular Mass
2,045.0793
Species
Human
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 4 2.5 2 4.6 3.2 2.3 6.1 4.1 3 15 15 15
y13 (1+) 4.4 2 3 7.3 3.6 2.9 8.5 4.1 4.2 15 15 15
y5 (1+) 4.8 3.2 2.8 4.1 3.7 2.8 6.3 4.9 4 15 15 15
sum 3.6 2.5 2.1 4.3 3.2 2.4 5.6 4.1 3.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments