CAMKK1, CPTAC-6029 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CAMKK1	CAMKKA

Sequence Length (AA)	Molecular Weight (Da)
505	55735

Protein Name
Calcium/calmodulin-dependent protein kinase kinase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEGGPAVCCQ	DPRAELVERV	AAIDVTHLEE	ADGGPEPTRN	GVDPPPRARA
60	70	80	90	100
ASVIPGSTSR	LLPARPSLSA	RKLSLQERPA	GSYLEAQAGP	YATGPASHIS
110	120	130	140	150
PRAWRRPTIE	SHHVAISDAE	DCVQLNQYKL	QSEIGKGAYG	VVRLAYNESE
160	170	180	190	200
DRHYAMKVLS	KKKLLKQYGF	PRRPPPRGSQ	AAQGGPAKQL	LPLERVYQEI
210	220	230	240	250
AILKKLDHVN	VVKLIEVLDD	PAEDNLYLVF	DLLRKGPVME	VPCDKPFSEE
260	270	280	290	300
QARLYLRDVI	LGLEYLHCQK	IVHRDIKPSN	LLLGDDGHVK	IADFGVSNQF
310	320	330	340	350
EGNDAQLSST	AGTPAFMAPE	AISDSGQSFS	GKALDVWATG	VTLYCFVYGK
360	370	380	390	400
CPFIDDFILA	LHRKIKNEPV	VFPEEPEISE	ELKDLILKML	DKNPETRIGV
410	420	430	440	450
PDIKLHPWVT	KNGEEPLPSE	EEHCSVVEVT	EEEVKNSVRL	IPSWTTVILV
460	470	480	490	500
KSMLRKRSFG	NPFEPQARRE	ERSMSAPGNL	LVKEGFGEGG	KSPELPGVQE
505
DEAAS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6029 Collapse assay details

Data source: Panorama

Official Gene Symbol: CAMKK1
Peptide Sequence: SFGNPFEPQAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 458
Peptide End: 468

CPTAC ID: non-CPTAC-6029
Peptide Molecular Mass: 1,248.5887
Species: Human
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	2.2	1.2	1	2	2	3.2	3	2.3	3.4	15	15	15
y4 (1+)	2	1.9	1.1	2.5	2.2	1.6	3.2	2.9	1.9	15	15	15
y5 (1+)	4.1	2.3	2.1	3	2.8	2.2	5.1	3.6	3	15	15	15
sum	2	1.5	0.9	1.9	1.9	2.1	2.8	2.4	2.3	15	15	15

CAMKK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6029 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CAMKK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6029 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments