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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
STAT5A STAT5
Sequence Length (AA) Molecular Weight (Da)
794 90647
Protein Name
Signal transducer and activator of transcription 5A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN
60 70 80 90 100
PQDRAQATQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQKTYDR
110 120 130 140 150
CPLELVRCIR HILYNEQRLV REANNCSSPA GILVDAMSQK HLQINQTFEE
160 170 180 190 200
LRLVTQDTEN ELKKLQQTQE YFIIQYQESL RIQAQFAQLA QLSPQERLSR
210 220 230 240 250
ETALQQKQVS LEAWLQREAQ TLQQYRVELA EKHQKTLQLL RKQQTIILDD
260 270 280 290 300
ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC
310 320 330 340 350
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA
360 370 380 390 400
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC
410 420 430 440 450
CVMEYHQATG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV
460 470 480 490 500
GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD
510 520 530 540 550
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNN SSSHLEDYSG
560 570 580 590 600
LSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK
610 620 630 640 650
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPERNL WNLKPFTTRD
660 670 680 690 700
FSIRSLADRL GDLSYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK
710 720 730 740 750
QVVPEFVNAS ADAGGSSATY MDQAPSPAVC PQAPYNMYPQ NPDHVLDQDG
760 770 780 790 794
EFDLDETMDV ARHVEELLRR PMDSLDSRLS PPAGLFTSAR GSLS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6252 Collapse assay details

Data source: Panorama

Official Gene Symbol
STAT5A
Peptide Modified Sequence
AVDGY[+79.966331]VKPQIK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
690
Peptide End
700
CPTAC ID
non-CPTAC-6252
Peptide Molecular Mass
1,296.6479
Species
Human
Assay Type
Enrichment PRM
Enrichment Method
immunoaffinity enrichment
Matrix
SKBR3 cells
Submitting Laboratory
Cell Signaling Technology
Submitting Lab PI
Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Q Exactive (Thermo)
Internal Standard
Heavy isotope-labeled peptides
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
EASY-nLC II (Thermo)
Column Packing
self-packed
Column Dimensions
ID 100um, length 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6 5.4 10.6 4.7 6.8 11.6 7.6 8.7 15.7 15 15 15
y8 (1+) 6.3 5 13.4 4.6 6.9 14.1 7.8 8.5 19.5 15 15 15
y9 (1+) 4.5 4.6 13.5 3.7 6.5 13.2 5.8 8 18.9 15 15 15
b5 (1+) 7.9 4.3 9.7 8.6 6.1 10.8 11.7 7.5 14.5 15 15 15
y9 (2+) 3.8 4.8 9.1 3.5 6.2 9.9 5.2 7.8 13.4 15 15 15
y8 (2+) 5.1 4.4 10 3.4 6.2 10.4 6.1 7.6 14.4 15 15 15
sum 4.3 4.6 10.7 3.1 6.2 11.3 5.3 7.7 15.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1270 Collapse assay details

Data source: Panorama

Official Gene Symbol
STAT5A
Peptide Sequence
ATIISEQQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
371
Peptide End
380
CPTAC ID
CPTAC-1270
Peptide Molecular Mass
1,087.5873
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 32 12.9 10.8 52.2 14.3 13.4 61.2 19.3 17.2 15 15 15
y6 (1+) 16.1 6 6.9 26.6 9.8 8.4 31.1 11.5 10.9 15 15 15
y7 (1+) 16.4 6.2 8.6 22.6 10.8 6.3 27.9 12.5 10.7 15 15 15
sum 8.1 5.8 6.9 19.6 9.9 7 21.2 11.5 9.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1728 Collapse assay details

Data source: Panorama

Official Gene Symbol
STAT5A
Peptide Modified Sequence
AVDGY[+80.0]VKPQIK
Modification Type
Phospho (Y)
Protein - Site of Modification
694
Peptide - Site of Modification
5
Peptide Start
690
Peptide End
700
CPTAC ID
CPTAC-1728
Peptide Molecular Mass
1,296.6479
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
immunoaffinity enrichment
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 19.1 9 3.9 20.5 9.3 5.7 28 12.9 6.9 15 15 15
y9 (2+) 9.5 6.6 6 11.3 7.6 4.2 14.8 10.1 7.3 15 15 15
y6 (1+) 17.6 5.9 7.4 26.9 5.6 9 32.1 8.1 11.7 15 15 15
sum 8.9 4.3 3.4 10.3 5.3 3.1 13.6 6.8 4.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1271 Collapse assay details

Data source: Panorama

Official Gene Symbol
STAT5A
Peptide Sequence
HILYNEQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
111
Peptide End
118
CPTAC ID
CPTAC-1271
Peptide Molecular Mass
1,071.5461
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 27.8 9.5 19.9 34.1 14.6 19.1 44 17.4 27.6 15 15 15
y6 (1+) 7.2 4.6 6.5 13.5 7 8.4 15.3 8.4 10.6 15 15 15
y7 (1+) 14 6.7 6 19.1 8.5 6.5 23.7 10.8 8.8 15 15 15
sum 7.9 4.2 6.4 13.6 7 7.8 15.7 8.2 10.1 15 15 15


Additional Resources and Comments