STAT5A, CPTAC-6252 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STAT5A	STAT5

Sequence Length (AA)	Molecular Weight (Da)
794	90647

Protein Name
Signal transducer and activator of transcription 5A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGWIQAQQL	QGDALRQMQV	LYGQHFPIEV	RHYLAQWIES	QPWDAIDLDN
60	70	80	90	100
PQDRAQATQL	LEGLVQELQK	KAEHQVGEDG	FLLKIKLGHY	ATQLQKTYDR
110	120	130	140	150
CPLELVRCIR	HILYNEQRLV	REANNCSSPA	GILVDAMSQK	HLQINQTFEE
160	170	180	190	200
LRLVTQDTEN	ELKKLQQTQE	YFIIQYQESL	RIQAQFAQLA	QLSPQERLSR
210	220	230	240	250
ETALQQKQVS	LEAWLQREAQ	TLQQYRVELA	EKHQKTLQLL	RKQQTIILDD
260	270	280	290	300
ELIQWKRRQQ	LAGNGGPPEG	SLDVLQSWCE	KLAEIIWQNR	QQIRRAEHLC
310	320	330	340	350
QQLPIPGPVE	EMLAEVNATI	TDIISALVTS	TFIIEKQPPQ	VLKTQTKFAA
360	370	380	390	400
TVRLLVGGKL	NVHMNPPQVK	ATIISEQQAK	SLLKNENTRN	ECSGEILNNC
410	420	430	440	450
CVMEYHQATG	TLSAHFRNMS	LKRIKRADRR	GAESVTEEKF	TVLFESQFSV
460	470	480	490	500
GSNELVFQVK	TLSLPVVVIV	HGSQDHNATA	TVLWDNAFAE	PGRVPFAVPD
510	520	530	540	550
KVLWPQLCEA	LNMKFKAEVQ	SNRGLTKENL	VFLAQKLFNN	SSSHLEDYSG
560	570	580	590	600
LSVSWSQFNR	ENLPGWNYTF	WQWFDGVMEV	LKKHHKPHWN	DGAILGFVNK
610	620	630	640	650
QQAHDLLINK	PDGTFLLRFS	DSEIGGITIA	WKFDSPERNL	WNLKPFTTRD
660	670	680	690	700
FSIRSLADRL	GDLSYLIYVF	PDRPKDEVFS	KYYTPVLAKA	VDGYVKPQIK
710	720	730	740	750
QVVPEFVNAS	ADAGGSSATY	MDQAPSPAVC	PQAPYNMYPQ	NPDHVLDQDG
760	770	780	790	794
EFDLDETMDV	ARHVEELLRR	PMDSLDSRLS	PPAGLFTSAR	GSLS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for non-CPTAC-6252 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT5A
Peptide Modified Sequence: AVDGY[+79.966331]VKPQIK
Modification Type: Phospho (Y)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 8
Peptide Start: 690
Peptide End: 700

CPTAC ID: non-CPTAC-6252
Peptide Molecular Mass: 1,296.6479
Species: Human
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: SKBR3 cells
Submitting Laboratory: Cell Signaling Technology
Submitting Lab PI: Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Q Exactive (Thermo)
Internal Standard: Heavy isotope-labeled peptides
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N V

LC: EASY-nLC II (Thermo)
Column Packing: self-packed
Column Dimensions: ID 100um, length 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	6	5.4	10.6	4.7	6.8	11.6	7.6	8.7	15.7	15	15	15
y8 (1+)	6.3	5	13.4	4.6	6.9	14.1	7.8	8.5	19.5	15	15	15
y9 (1+)	4.5	4.6	13.5	3.7	6.5	13.2	5.8	8	18.9	15	15	15
b5 (1+)	7.9	4.3	9.7	8.6	6.1	10.8	11.7	7.5	14.5	15	15	15
y9 (2+)	3.8	4.8	9.1	3.5	6.2	9.9	5.2	7.8	13.4	15	15	15
y8 (2+)	5.1	4.4	10	3.4	6.2	10.4	6.1	7.6	14.4	15	15	15
sum	4.3	4.6	10.7	3.1	6.2	11.3	5.3	7.7	15.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1270 Collapse assay details

Data source: Panorama

Official Gene Symbol: STAT5A
Peptide Sequence: ATIISEQQAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 371
Peptide End: 380

CPTAC ID: CPTAC-1270
Peptide Molecular Mass: 1,087.5873
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu