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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ANKRD27

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ANKRD27 N/A
Sequence Length (AA) Molecular Weight (Da)
1050 116984
Protein Name
Ankyrin repeat domain-containing protein 27
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST
60 70 80 90 100
CQFESYILIP VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF
110 120 130 140 150
YNEKEESFSI LCIAHPLEKR ESSEEPLAPS DPFSLKTIED VREFLGRHSE
160 170 180 190 200
RFDRNIASFH RTFRECERKS LRHHIDSANA LYTKCLQQLL RDSHLKMLAK
210 220 230 240 250
QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN KITRSLQDLQ
260 270 280 290 300
QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ
310 320 330 340 350
RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSLAKDELGY
360 370 380 390 400
CLTSFEAAIE YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC
410 420 430 440 450
LFKHIASGNQ KEVERLLSQE DHDKDTVQKM CHPLCFCDDC EKLVSGRLND
460 470 480 490 500
PSVVTPFSRD DRGHTPLHVA AVCGQASLID LLVSKGAMVN ATDYHGATPL
510 520 530 540 550
HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG HEDCVKALVY
560 570 580 590 600
YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET
610 620 630 640 650
PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS
660 670 680 690 700
FSSMSASSRQ EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE
710 720 730 740 750
DTVSAADPEF CHPLCQCPKC APAQKRLAKV PASGLGVNVT SQDGSSPLHV
760 770 780 790 800
AALHGRADLI PLLLKHGANA GARNADQAVP LHLACQQGHF QVVKCLLDSN
810 820 830 840 850
AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN NKGNTALHEA
860 870 880 890 900
VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV
910 920 930 940 950
ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG
960 970 980 990 1000
KTSREIMARD RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS
1010 1020 1030 1040 1050
DWPERPGLTQ TGPGHRRMLR RHTVEDAVVS QGPEAAGPLS TPQEVSASRS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1198 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANKRD27
Peptide Sequence
LNDPSVVTPFSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
448
Peptide End
459
CPTAC ID
CPTAC-1198
Peptide Molecular Mass
1,330.6881
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 22.5 6 5.7 18.6 9.6 8.2 29.2 11.3 10 15 15 15
y6 (1+) 19.8 8.5 5.7 19.8 8.2 7.5 28 11.8 9.4 15 15 15
y9 (1+) 14.1 7.2 5.9 17.1 9.3 7.7 22.2 11.8 9.7 15 15 15
sum 8 5.9 3.9 10.4 6.9 6 13.1 9.1 7.2 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y9 (1+) 3.33 3.56 5.59 3.52 3.66 4.88
y6 (1+) 3.3 3.3 4.91 3.5 3.61 4.52
y5 (1+) 3.14 2.98 4 3.48 3.73 4.95
sum 3.28 3.32 4.92 3.49 3.65 4.75

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y9 (1+) 41.5 40.1 57.7
y6 (1+) 46.5 46.7 65.9
y5 (1+) 43.4 42.5 60.7
sum 41.3 41.1 58.3

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1175 Collapse assay details

Data source: Panorama

Official Gene Symbol
ANKRD27
Peptide Sequence
LYDLPDEPFTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
927
Peptide End
937
CPTAC ID
CPTAC-1175
Peptide Molecular Mass
1,364.6612
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 18.4 15.8 23.4 20.3 21 22.4 27.4 26.3 32.4 15 15 15
y7 (1+) 9.1 2.6 4.1 9.2 7.4 6.7 12.9 7.8 7.9 15 15 15
y9 (1+) 14.1 5.6 11.3 14.2 7.5 11.6 20 9.4 16.2 15 15 15
sum 7.6 2 4.2 7.9 6.7 5.8 11 7 7.2 15 15 15

Selectivity

Data source: Panorama

view larger image
Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y9 (1+) 11.71 15.32 22.85 17.88 18.75 20.38
y7 (1+) 11.55 12.67 21.83 17.53 19.16 24.2
y5 (1+) 17.66 14.55 20.98 17.23 22.66 35.95
sum 11.72 13.12 21.92 17.52 19.27 23.84

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y5 (1+) 35.1 16.4 10.3 6.7 15
y7 (1+) 7.4 8.5 1.9 7.4 8.2
y9 (1+) 11.7 9.1 9.2 7.3 16
sum 6 6.3 1.6 6.4 3
Fragment ion / Transition all_intra_CV all_inter_CV
y5 (1+) 16.6 16
y7 (1+) 7 6.3
y9 (1+) 10.4 11.4
sum 4.9 5.2

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
y9 (1+) 42 91 100.2
y7 (1+) 40.4 78.1 87.9
y5 (1+) 50.9 78.3 93.4
sum 37.1 78.1 86.5

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments