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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C3AR1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C3AR1 AZ3B, C3R1, HNFAG09
Sequence Length (AA) Molecular Weight (Da)
482 53864
Protein Name
C3a anaphylatoxin chemotactic receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASFSAETNS TDLLSQPWNE PPVILSMVIL SLTFLLGLPG NGLVLWVAGL
60 70 80 90 100
KMQRTVNTIW FLHLTLADLL CCLSLPFSLA HLALQGQWPY GRFLCKLIPS
110 120 130 140 150
IIVLNMFASV FLLTAISLDR CLVVFKPIWC QNHRNVGMAC SICGCIWVVA
160 170 180 190 200
FVMCIPVFVY REIFTTDNHN RCGYKFGLSS SLDYPDFYGD PLENRSLENI
210 220 230 240 250
VQPPGEMNDR LDPSSFQTND HPWTVPTVFQ PQTFQRPSAD SLPRGSARLT
260 270 280 290 300
SQNLYSNVFK PADVVSPKIP SGFPIEDHET SPLDNSDAFL STHLKLFPSA
310 320 330 340 350
SSNSFYESEL PQGFQDYYNL GQFTDDDQVP TPLVAITITR LVVGFLLPSV
360 370 380 390 400
IMIACYSFIV FRMQRGRFAK SQSKTFRVAV VVVAVFLVCW TPYHIFGVLS
410 420 430 440 450
LLTDPETPLG KTLMSWDHVC IALASANSCF NPFLYALLGK DFRKKARQSI
460 470 480 482
QGILEAAFSE ELTRSTHCPS NNVISERNST TV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1177 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3AR1
Peptide Modified Sequence
STHC[+57.0]PSNNVISER
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
468
Peptide - Site of Modification
4
Peptide Start
465
Peptide End
477
CPTAC ID
CPTAC-1177
Peptide Molecular Mass
1,499.6787
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 28.3 12.3 8.1 29 11.4 9.5 40.5 16.8 12.5 15 15 15
y4 (1+) 17.1 9.8 8.3 16.4 11.5 7.9 23.7 15.1 11.5 15 15 15
y5 (1+) 56 30.2 21.5 55.8 30.2 21 79.1 42.7 30.1 15 15 15
sum 19.1 7.8 6.3 16.1 9.4 6.5 25 12.2 9.1 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y4 (1+) 3.69 4.23 5.29 4.6 4.55 5.07
y3 (1+) 4.64 5.43 6.63 4.24 4.91 4.75
y5 (1+) 4.41 4.64 5.13 3.72 3.56 3.8
sum 3.92 4.44 5.53 4.43 4.53 4.87

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y5 (1+) 22.9 22 9.5 47.8 7
y4 (1+) 6.5 6 3.6 11.5 3.6
y3 (1+) 14 10.9 2.6 9.4 9.8
sum 6.4 5.9 3.1 7.3 4.9
Fragment ion / Transition all_intra_CV all_inter_CV
y5 (1+) 21.9 27.3
y4 (1+) 6.2 8.7
y3 (1+) 9.6 11.1
sum 5.6 5.9

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y4 (1+) 39 45 59.5
y5 (1+) 78.3 72.6 106.8
y3 (1+) 47.9 47.4 67.4
sum 43.1 43.6 61.3

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments