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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C3AR1 AZ3B, C3R1, HNFAG09
Sequence Length (AA) Molecular Weight (Da)
482 53864
Protein Name
C3a anaphylatoxin chemotactic receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASFSAETNS TDLLSQPWNE PPVILSMVIL SLTFLLGLPG NGLVLWVAGL
60 70 80 90 100
KMQRTVNTIW FLHLTLADLL CCLSLPFSLA HLALQGQWPY GRFLCKLIPS
110 120 130 140 150
IIVLNMFASV FLLTAISLDR CLVVFKPIWC QNHRNVGMAC SICGCIWVVA
160 170 180 190 200
FVMCIPVFVY REIFTTDNHN RCGYKFGLSS SLDYPDFYGD PLENRSLENI
210 220 230 240 250
VQPPGEMNDR LDPSSFQTND HPWTVPTVFQ PQTFQRPSAD SLPRGSARLT
260 270 280 290 300
SQNLYSNVFK PADVVSPKIP SGFPIEDHET SPLDNSDAFL STHLKLFPSA
310 320 330 340 350
SSNSFYESEL PQGFQDYYNL GQFTDDDQVP TPLVAITITR LVVGFLLPSV
360 370 380 390 400
IMIACYSFIV FRMQRGRFAK SQSKTFRVAV VVVAVFLVCW TPYHIFGVLS
410 420 430 440 450
LLTDPETPLG KTLMSWDHVC IALASANSCF NPFLYALLGK DFRKKARQSI
460 470 480 482
QGILEAAFSE ELTRSTHCPS NNVISERNST TV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1177 Collapse assay details

Data source: Panorama

Official Gene Symbol
C3AR1
Peptide Modified Sequence
STHC[+57.0]PSNNVISER
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
468
Peptide - Site of Modification
4
Peptide Start
465
Peptide End
477
CPTAC ID
CPTAC-1177
Peptide Molecular Mass
1,499.6787
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK
CPTAC-1169:
MELK.
DLKPENLLFDEYHK
CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR
CPTAC-1172:
PTPRC.
LFLAEFQSIPR
CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK
CPTAC-1175:
ANKRD27.
LYDLPDEPFTR
CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER
CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R
CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK
CPTAC-1181:
CYBB.
LLGSALALAR
CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK
CPTAC-1184:
GPR20.
YLAIVRPEGSR
CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR
CPTAC-1187:
TOP2A.
TLAVSGLGVVGR
CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR
CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK
CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R
CPTAC-1193:
PES1.
FLLHEPIVNK
CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR
CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R
CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR
CPTAC-1199:
CD48.
LQVLDPVPKPVIK
CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK
CPTAC-1202:
COL11A1.
GEVGQIGPR
CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR
CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR
CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK
CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR
CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR
CPTAC-1211:
MCM10.
IGGETLLPR
CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR
CPTAC-1214:
SFTPA1.
NPEENEAIASFVK
CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK
CPTAC-1217:
ANXA11.
TPVLFDIYEIK
CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 28.3 12.3 8.1 29 11.4 9.5 40.5 16.8 12.5 15 15 15
y4 (1+) 17.1 9.8 8.3 16.4 11.5 7.9 23.7 15.1 11.5 15 15 15
y5 (1+) 56 30.2 21.5 55.8 30.2 21 79.1 42.7 30.1 15 15 15
sum 19.1 7.8 6.3 16.1 9.4 6.5 25 12.2 9.1 15 15 15

Additional Resources and Comments