TARS, CPTAC-1195 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TARS	N/A

Sequence Length (AA)	Molecular Weight (Da)
723	83435

Protein Name
Threonine--tRNA ligase, cytoplasmic

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MFEEKASSPS	GKMGGEEKPI	GAGEEKQKEG	GKKKNKEGSG	DGGRAELNPW
60	70	80	90	100
PEYIYTRLEM	YNILKAEHDS	ILAEKAEKDS	KPIKVTLPDG	KQVDAESWKT
110	120	130	140	150
TPYQIACGIS	QGLADNTVIA	KVNNVVWDLD	RPLEEDCTLE	LLKFEDEEAQ
160	170	180	190	200
AVYWHSSAHI	MGEAMERVYG	GCLCYGPPIE	NGFYYDMYLE	EGGVSSNDFS
210	220	230	240	250
SLEALCKKII	KEKQAFERLE	VKKETLLAMF	KYNKFKCRIL	NEKVNTPTTT
260	270	280	290	300
VYRCGPLIDL	CRGPHVRHTG	KIKALKIHKN	SSTYWEGKAD	METLQRIYGI
310	320	330	340	350
SFPDPKMLKE	WEKFQEEAKN	RDHRKIGRDQ	ELYFFHELSP	GSCFFLPKGA
360	370	380	390	400
YIYNALIEFI	RSEYRKRGFQ	EVVTPNIFNS	RLWMTSGHWQ	HYSENMFSFE
410	420	430	440	450
VEKELFALKP	MNCPGHCLMF	DHRPRSWREL	PLRLADFGVL	HRNELSGALT
460	470	480	490	500
GLTRVRRFQQ	DDAHIFCAME	QIEDEIKGCL	DFLRTVYSVF	GFSFKLNLST
510	520	530	540	550
RPEKFLGDIE	VWDQAEKQLE	NSLNEFGEKW	ELNSGDGAFY	GPKIDIQIKD
560	570	580	590	600
AIGRYHQCAT	IQLDFQLPIR	FNLTYVSHDG	DDKKRPVIVH	RAILGSVERM
610	620	630	640	650
IAILTENYGG	KWPFWLSPRQ	VMVVPVGPTC	DEYAQKVRQQ	FHDAKFMADI
660	670	680	690	700
DLDPGCTLNK	KIRNAQLAQY	NFILVVGEKE	KISGTVNIRT	RDNKVHGERT
710	720	723
ISETIERLQQ	LKEFRSKQAE	EEF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-278 Collapse assay details

Data source: Panorama

Official Gene Symbol: TARS
Peptide Sequence: GAYIYNALIEFIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 349
Peptide End: 361

CPTAC ID: CPTAC-278
Peptide Molecular Mass: 1,541.8242
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168: FCER1G. AAITSYEK	CPTAC-1169: MELK. DLKPENLLFDEYHK	CPTAC-1170: PEPD. VPLALFALNR
CPTAC-1171: PES1. LAALSASLAR	CPTAC-1172: PTPRC. LFLAEFQSIPR	CPTAC-1173: SYK. NYLGGFALSVAHGR
CPTAC-1174: SYK. LIATTAHEK	CPTAC-1175: ANKRD27. LYDLPDEPFTR	CPTAC-1176: ASGR2. ADHDALLFHLK
CPTAC-1177: C3AR1. STHC[+57.0]PSNNVISER	CPTAC-1178: CD79A. SHGGIYVC[+57.0]R	CPTAC-1179: CEACAM1. TIIVTELSPVVAKPQIK
CPTAC-1180: COL3A1. GPVGPSGPPGK	CPTAC-1181: CYBB. LLGSALALAR	CPTAC-1182: EVI2B. STLLADDLEIK
CPTAC-1183: FCGR2B. VTFFQNGK	CPTAC-1184: GPR20. YLAIVRPEGSR	CPTAC-1185: SIX6. AIVAFHGGNYR
CPTAC-1186: THBS2. GTLLALEGPGLSQR	CPTAC-1187: TOP2A. TLAVSGLGVVGR	CPTAC-1188: TSPAN14. TLISDIEAVK
CPTAC-1189: TLE1. FTIPESLDR	CPTAC-1190: SLC7A9. LGSYVQNIFTAAK	CPTAC-1191: SLC7A9. VLSYISVR
CPTAC-1192: PEPD. FEVNNTILHPEIVEC[+57.0]R	CPTAC-1193: PES1. FLLHEPIVNK	CPTAC-1194: TARS. AELNPWPEYIYTR
CPTAC-1195: TARS. GFQEVVTPNIFNSR	CPTAC-1196: STARD3NL. VLILAYAVC[+57.0]R	CPTAC-1197: STARD3NL. LLIVQDASER
CPTAC-1198: ANKRD27. LNDPSVVTPFSR	CPTAC-1199: CD48. LQVLDPVPKPVIK	CPTAC-1200: CDK1. LESEEEGVPSTAIR
CPTAC-1201: COL11A1. GFDGLPGLPGDK	CPTAC-1202: COL11A1. GEVGQIGPR	CPTAC-1203: CYBB. IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204: DLGAP5. DVNIPTLEGR	CPTAC-1205: DLGAP5. IAQPHHGVPYFR	CPTAC-1206: ETS1. YENDYPSVILR
CPTAC-1207: EVI2B. STPGFILDTTSNK	CPTAC-1208: LYL1. DQAAALAAGPTPPGPR	CPTAC-1209: LYL1. SQPAPPADPDGSPGGAARPIK
CPTAC-1210: MCM10. GTNLIIQETR	CPTAC-1211: MCM10. IGGETLLPR	CPTAC-1212: OSGIN1. AVDDPGLVFNQLPK
CPTAC-1213: PIK3CD. GELLNPTGTVR	CPTAC-1214: SFTPA1. NPEENEAIASFVK	CPTAC-1215: ZNF507. TKPDPLIHVIQK
CPTAC-1216: ZNF592. AAPLIVEVFNK	CPTAC-1217: ANXA11. TPVLFDIYEIK	CPTAC-1218: ANXA11. DAQELYAAGENR

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	12.9	10.5	10.7	29	23.4	26.1	31.7	25.6	28.2	25	15	15
y8 (1+)	13.3	10.6	9.8	27.2	24.2	27.4	30.3	26.4	29.1	25	15	15
y9 (1+)	9.2	6.1	9.8	27.3	22.8	26.9	28.8	23.6	28.6	25	15	15
sum	9.3	7.7	9.6	26.9	23.1	26.7	28.5	24.3	28.4	25	15	15

	Slope of Curve Fit for cell line
fragment_ion	1	2	3	4	5	6
y8 (1+)	4.81	12.02	13.12	23.71	20.28	27.6
y9 (1+)	5.23	13.1	20.18	19.61	25.56	21.15
y7 (1+)	5.31	13.44	14.93	20.46	23.46	25.02
sum	5.24	13.16	16.8	20.2	23.85	23.08

Fragment ion / Transition	intra_CV	inter_CV	total_CV
y7 (1+)	37.1	93.6	100.7
y8 (1+)	38.1	90.7	98.4
y9 (1+)	40.9	94.6	103.1
sum	38.8	93.7	101.4

	Slope of Curve Fit for cell line
fragment_ion	1	2	3	4	5	6
y9 (1+)	2.06	2.27	4.3	3.73	3.57	4.33
y7 (1+)	1.98	2.14	4.04	3.51	3.01	5.36
y8 (1+)	2.32	2.18	4.01	3.39	3.11	4.68
sum	2.11	2.18	4.1	3.51	3.2	4.68

Fragment ion / Transition	control_intra_CV	actual_temp_intra_CV	frozen_intra_CV	FTx1_intra_CV	FTx2_intra_CV
y7 (1+)	19.2	15.9	3	10.4	8.3
y9 (1+)	5.8	8.4	2.2	15.9	5.9
y8 (1+)	16.5	17.2	2.7	12.4	11
sum	9	8.1	1.3	12.3	6.7

Fragment ion / Transition	intra_CV	inter_CV	total_CV
y7 (1+)	37.4	81.6	89.8
y9 (1+)	37.3	80.4	88.6
y8 (1+)	38.8	82	90.7
sum	37.7	81.2	89.5

Fragment ion / Transition	all_intra_CV	all_inter_CV
y7 (1+)	12.1	14.1
y9 (1+)	7.8	9.5
y8 (1+)	12.8	9.2
sum	7.6	6.9

Search form

TARS

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-278 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-279 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-1194 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Selectivity

Stability

Endogenous

Additional Resources and Comments

Comments

Assay Details for CPTAC-1195 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Selectivity

Stability

Endogenous

Additional Resources and Comments

Comments