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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PEPD PRD
Sequence Length (AA) Molecular Weight (Da)
493 54548
Protein Name
Xaa-Pro dipeptidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAATGPSFW LGNETLKVPL ALFALNRQRL CERLRKNPAV QAGSIVVLQG
60 70 80 90 100
GEETQRYCTD TGVLFRQESF FHWAFGVTEP GCYGVIDVDT GKSTLFVPRL
110 120 130 140 150
PASHATWMGK IHSKEHFKEK YAVDDVQYVD EIASVLTSQK PSVLLTLRGV
160 170 180 190 200
NTDSGSVCRE ASFDGISKFE VNNTILHPEI VECRVFKTDM ELEVLRYTNK
210 220 230 240 250
ISSEAHREVM KAVKVGMKEY ELESLFEHYC YSRGGMRHSS YTCICGSGEN
260 270 280 290 300
SAVLHYGHAG APNDRTIQNG DMCLFDMGGE YYCFASDITC SFPANGKFTA
310 320 330 340 350
DQKAVYEAVL RSSRAVMGAM KPGVWWPDMH RLADRIHLEE LAHMGILSGS
360 370 380 390 400
VDAMVQAHLG AVFMPHGLGH FLGIDVHDVG GYPEGVERID EPGLRSLRTA
410 420 430 440 450
RHLQPGMVLT VEPGIYFIDH LLDEALADPA RASFLNREVL QRFRGFGGVR
460 470 480 490 493
IEEDVVVTDS GIELLTCVPR TVEEIEACMA GCDKAFTPFS GPK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1192 Collapse assay details

Data source: Panorama

Official Gene Symbol
PEPD
Peptide Modified Sequence
FEVNNTILHPEIVEC[+57.0]R
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
183
Peptide - Site of Modification
15
Peptide Start
169
Peptide End
184
CPTAC ID
CPTAC-1192
Peptide Molecular Mass
1,968.9727
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK
CPTAC-1169:
MELK.
DLKPENLLFDEYHK
CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR
CPTAC-1172:
PTPRC.
LFLAEFQSIPR
CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK
CPTAC-1175:
ANKRD27.
LYDLPDEPFTR
CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER
CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R
CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK
CPTAC-1181:
CYBB.
LLGSALALAR
CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK
CPTAC-1184:
GPR20.
YLAIVRPEGSR
CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR
CPTAC-1187:
TOP2A.
TLAVSGLGVVGR
CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR
CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK
CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R
CPTAC-1193:
PES1.
FLLHEPIVNK
CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR
CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R
CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR
CPTAC-1199:
CD48.
LQVLDPVPKPVIK
CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK
CPTAC-1202:
COL11A1.
GEVGQIGPR
CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR
CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR
CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK
CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR
CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR
CPTAC-1211:
MCM10.
IGGETLLPR
CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR
CPTAC-1214:
SFTPA1.
NPEENEAIASFVK
CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK
CPTAC-1217:
ANXA11.
TPVLFDIYEIK
CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 38.3 33.2 27.9 44.9 22.1 30.6 59 39.9 41.4 15 15 15
y7 (1+) 28.3 12.6 12.1 26.1 13.8 20.2 38.5 18.7 23.5 15 15 15
y8 (1+) 51.8 19.6 23.6 77.5 24.8 23.6 93.2 31.6 33.4 15 15 15
sum 16.2 10.1 13 14.6 12.8 18.5 21.8 16.3 22.6 15 15 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y7 (1+) 0.64 0.56 1.42 1.37 1.19 1.72
y8 (1+) 0.46 0.52 2.88 0.87 1.42 1.33
y5 (1+) 0.41 0.75 2.19 1.12 1.42 2.54
sum 0.58 0.56 1.46 1.19 1.23 1.64

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y8 (1+) 34.7 19.6 13 37.6 23.1
y7 (1+) 22.4 16.8 12.7 15.9 20.1
y5 (1+) 149.6 35.3 17.4 35.9 45.8
sum 19.4 14.9 11.3 14.6 14.2
Fragment ion / Transition all_intra_CV all_inter_CV
y8 (1+) 24.6 33.3
y7 (1+) 17.5 18.7
y5 (1+) 53.2 85.1
sum 14.9 15.1

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y5 (1+) 35.6 83.1 90.4
y7 (1+) 44.9 93.2 103.5
y8 (1+) 40.7 90.6 99.3
sum 37.6 87.8 95.5

Additional Resources and Comments


Assay Details for CPTAC-1170 Collapse assay details

Data source: Panorama

Official Gene Symbol
PEPD
Peptide Sequence
VPLALFALNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
18
Peptide End
27
CPTAC ID
CPTAC-1170
Peptide Molecular Mass
1,112.6706
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK
CPTAC-1169:
MELK.
DLKPENLLFDEYHK
CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR
CPTAC-1172:
PTPRC.
LFLAEFQSIPR
CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK
CPTAC-1175:
ANKRD27.
LYDLPDEPFTR
CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER
CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R
CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK
CPTAC-1181:
CYBB.
LLGSALALAR
CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK
CPTAC-1184:
GPR20.
YLAIVRPEGSR
CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR
CPTAC-1187:
TOP2A.
TLAVSGLGVVGR
CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR
CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK
CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R
CPTAC-1193:
PES1.
FLLHEPIVNK
CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR
CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R
CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR
CPTAC-1199:
CD48.
LQVLDPVPKPVIK
CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK
CPTAC-1202:
COL11A1.
GEVGQIGPR
CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR
CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR
CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK
CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR
CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR
CPTAC-1211:
MCM10.
IGGETLLPR
CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR
CPTAC-1214:
SFTPA1.
NPEENEAIASFVK
CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK
CPTAC-1217:
ANXA11.
TPVLFDIYEIK
CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.7 7.1 5.7 10.3 10.8 6.6 12.9 12.9 8.7 15 15 15
y6 (1+) 13.7 6.4 6.8 14.3 8.5 8.1 19.8 10.6 10.6 15 15 15
y7 (1+) 10.1 4.2 4.1 10.1 7.9 5.7 14.3 8.9 7 15 15 15
sum 6.5 4.7 3.2 7.5 8.2 5.1 9.9 9.5 6 15 15 15

Selectivity

Data source: Panorama

Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y6 (1+) 3.55 3.72 7.02 5.81 5.24 6.61
y7 (1+) 3.33 3.52 7.17 5.71 5.54 6.94
y5 (1+) 3.23 3.56 7.49 5.36 5.2 6.49
sum 3.35 3.58 7.22 5.62 5.35 6.69

Stability

Data source: Panorama

Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
Fragment ion / Transition all_intra_CV all_inter_CV

Endogenous

Data source: Panorama

Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 38.7 44.5 59
y6 (1+) 40.3 45.9 61.1
y5 (1+) 38.6 43.7 58.3
sum 39 44.6 59.2

Additional Resources and Comments