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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ZNF592

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ZNF592 KIAA0211
Sequence Length (AA) Molecular Weight (Da)
1267 137528
Protein Name
Zinc finger protein 592
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGDMKTPDFD DLLAAFDIPD PTSLDAKEAI QTPSEENESP LKPPGICMDE
60 70 80 90 100
SVSLSHSGSA PDVPAVSVIV KNTSRQESFE AEKDHITPSL LHNGFRGSDL
110 120 130 140 150
PPDPHNCGKF DSTFMNGDSA RSFPGKLEPP KSEPLPTFNQ FSPISSPEPE
160 170 180 190 200
DPIKDNGFGI KPKHSDSYFP PPLGCGAVGG PVLEALAKFP VPELHMFDHF
210 220 230 240 250
CKKEPKPEPL PLGSQQEHEQ SGQNTVEPHK DPDATRFFGE ALEFNSHPSN
260 270 280 290 300
SIGESKGLAR ELGTCSSVPP RQRLKPAHSK LSSCVAALVA LQAKRVASVT
310 320 330 340 350
KEDQPGHTKD LSGPTKESSK GSPKMPKSPK SPRSPLEATR KSIKPSDSPR
360 370 380 390 400
SICSDSSSKG SPSVAASSPP AIPKVRIKTI KTSSGEIKRT VTRILPDPDD
410 420 430 440 450
PSKSPVGSPL GSAIAEAPSE MPGDEVPVEE HFPEAGTNSG SPQGARKGDE
460 470 480 490 500
SMTKASDSSS PSCSSGPRVP KGAAPGSQTG KKQQSTALQA STLAPANLLP
510 520 530 540 550
KAVHLANLNL VPHSVAASVT AKSSVQRRSQ PQLTQMSVPL VHQVKKAAPL
560 570 580 590 600
IVEVFNKVLH SSNPVPLYAP NLSPPADSRI HVPASGYCCL ECGDAFALEK
610 620 630 640 650
SLSQHYGRRS VHIEVLCTLC SKTLLFFNKC SLLRHARDHK SKGLVMQCSQ
660 670 680 690 700
LLVKPISADQ MFVSAPVNST APAAPAPSSS PKHGLTSGSA SPPPPALPLY
710 720 730 740 750
PDPVRLIRYS IKCLECHKQM RDYMVLAAHF QRTTEETEGL TCQVCQMLLP
760 770 780 790 800
NQCSFCAHQR IHAHKSPYCC PECGVLCRSA YFQTHVKENC LHYARKVGYR
810 820 830 840 850
CIHCGVVHLT LALLKSHIQE RHCQVFHKCA FCPMAFKTAS STADHSATQH
860 870 880 890 900
PTQPHRPSQL IYKCSCEMVF NKKRHIQQHF YQNVSKTQVG VFKCPECPLL
910 920 930 940 950
FVQKPELMQH VKSTHGVPRN VDELSSLQSS ADTSSSRPGS RVPTEPPATS
960 970 980 990 1000
VAARSSSLPS GRWGRPEAHR RVEARPRLRN TGWTCQECQE WVPDRESYVS
1010 1020 1030 1040 1050
HMKKSHGRTL KRYPCRQCEQ SFHTPNSLRK HIRNNHDTVK KFYTCGYCTE
1060 1070 1080 1090 1100
DSPSFPRPSL LESHISLMHG IRNPDLSQTS KVKPPGGHSP QVNHLKRPVS
1110 1120 1130 1140 1150
GVGDAPGTSN GATVSSTKRH KSLFQCAKCS FATDSGLEFQ SHIPQHQVDS
1160 1170 1180 1190 1200
STAQCLLCGL CYTSASSLSR HLFIVHKVRD QEEEEEEEAA AAEMAVEVAE
1210 1220 1230 1240 1250
PEEGSGEEVP METRENGLEE CAGEPLSADP EARRLLGPAP EDDGGHNDHS
1260 1267
QPQASQDQDS HTLSPQV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1216 Collapse assay details

Data source: Panorama

Official Gene Symbol
ZNF592
Peptide Sequence
AAPLIVEVFNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
547
Peptide End
557
CPTAC ID
CPTAC-1216
Peptide Molecular Mass
1,199.6914
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM

CPTAC-1168:
FCER1G.
AAITSYEK 		CPTAC-1169:
MELK.
DLKPENLLFDEYHK 		CPTAC-1170:
PEPD.
VPLALFALNR
CPTAC-1171:
PES1.
LAALSASLAR 		CPTAC-1172:
PTPRC.
LFLAEFQSIPR 		CPTAC-1173:
SYK.
NYLGGFALSVAHGR
CPTAC-1174:
SYK.
LIATTAHEK 		CPTAC-1175:
ANKRD27.
LYDLPDEPFTR 		CPTAC-1176:
ASGR2.
ADHDALLFHLK
CPTAC-1177:
C3AR1.
STHC[+57.0]PSNNVISER 		CPTAC-1178:
CD79A.
SHGGIYVC[+57.0]R 		CPTAC-1179:
CEACAM1.
TIIVTELSPVVAKPQIK
CPTAC-1180:
COL3A1.
GPVGPSGPPGK 		CPTAC-1181:
CYBB.
LLGSALALAR 		CPTAC-1182:
EVI2B.
STLLADDLEIK
CPTAC-1183:
FCGR2B.
VTFFQNGK 		CPTAC-1184:
GPR20.
YLAIVRPEGSR 		CPTAC-1185:
SIX6.
AIVAFHGGNYR
CPTAC-1186:
THBS2.
GTLLALEGPGLSQR 		CPTAC-1187:
TOP2A.
TLAVSGLGVVGR 		CPTAC-1188:
TSPAN14.
TLISDIEAVK
CPTAC-1189:
TLE1.
FTIPESLDR 		CPTAC-1190:
SLC7A9.
LGSYVQNIFTAAK 		CPTAC-1191:
SLC7A9.
VLSYISVR
CPTAC-1192:
PEPD.
FEVNNTILHPEIVEC[+57.0]R 		CPTAC-1193:
PES1.
FLLHEPIVNK 		CPTAC-1194:
TARS.
AELNPWPEYIYTR
CPTAC-1195:
TARS.
GFQEVVTPNIFNSR 		CPTAC-1196:
STARD3NL.
VLILAYAVC[+57.0]R 		CPTAC-1197:
STARD3NL.
LLIVQDASER
CPTAC-1198:
ANKRD27.
LNDPSVVTPFSR 		CPTAC-1199:
CD48.
LQVLDPVPKPVIK 		CPTAC-1200:
CDK1.
LESEEEGVPSTAIR
CPTAC-1201:
COL11A1.
GFDGLPGLPGDK 		CPTAC-1202:
COL11A1.
GEVGQIGPR 		CPTAC-1203:
CYBB.
IGVFLC[+57.0]GPEALAETLSK
CPTAC-1204:
DLGAP5.
DVNIPTLEGR 		CPTAC-1205:
DLGAP5.
IAQPHHGVPYFR 		CPTAC-1206:
ETS1.
YENDYPSVILR
CPTAC-1207:
EVI2B.
STPGFILDTTSNK 		CPTAC-1208:
LYL1.
DQAAALAAGPTPPGPR 		CPTAC-1209:
LYL1.
SQPAPPADPDGSPGGAARPIK
CPTAC-1210:
MCM10.
GTNLIIQETR 		CPTAC-1211:
MCM10.
IGGETLLPR 		CPTAC-1212:
OSGIN1.
AVDDPGLVFNQLPK
CPTAC-1213:
PIK3CD.
GELLNPTGTVR 		CPTAC-1214:
SFTPA1.
NPEENEAIASFVK 		CPTAC-1215:
ZNF507.
TKPDPLIHVIQK
CPTAC-1216:
ZNF592.
AAPLIVEVFNK 		CPTAC-1217:
ANXA11.
TPVLFDIYEIK 		CPTAC-1218:
ANXA11.
DAQELYAAGENR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 29.9 15.4 10 36.2 17.3 12.8 47 23.2 16.2 15 15 15
y6 (1+) 13.2 11 7 11.4 11.1 8.1 17.4 15.6 10.7 15 15 15
y7 (1+) 10.8 10.4 11.5 19.6 12.3 9.4 22.4 16.1 14.9 15 15 15
sum 8.5 5 5.7 7.8 7.3 4.2 11.5 8.8 7.1 15 15 15

Selectivity

Data source: Panorama

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Slope of Curve Fit for cell line
fragment_ion 1 2 3 4 5 6
y7 (1+) 3.98 4.15 7.6 4.64 5.1 6.19
y5 (1+) 4.99 6.58 8.97 6.96 6.93 9.52
y6 (1+) 4.17 4.11 6.69 5.21 4.86 6.36
sum 4.16 4.37 7.31 5.17 5.17 6.55

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y5 (1+) 28.5 23 21.5 18.9 17.3
y6 (1+) 8.4 15 1.2 1 12.6
y7 (1+) 9.9 11.2 4.1 8.4 16.3
sum 8.2 10.7 4.2 5.5 14.5
Fragment ion / Transition all_intra_CV all_inter_CV
y5 (1+) 22 32.8
y6 (1+) 8.9 10.8
y7 (1+) 10.2 11.5
sum 9 11.4

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 38.7 40.7 56.2
y6 (1+) 39 42 57.3
y5 (1+) 46.2 50 68.1
sum 36.9 38.8 53.5

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
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