Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT1 PKB,RAC
Sequence Length (AA) Molecular Weight (Da)
480 55686
Protein Name
RAC-alpha serine/threonine-protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA
60 70 80 90 100
PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT
110 120 130 140 150
TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF
160 170 180 190 200
EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR
210 220 230 240 250
VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA
260 270 280 290 300
RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
310 320 330 340 350
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY
360 370 380 390 400
NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK
410 420 430 440 450
EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT
460 470 480
PPDQDDSMEC VDSERRPHFP QFSYSASGTA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-783 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1
Peptide Sequence
FFAGIVWQHVYEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
407
Peptide End
419
CPTAC ID
CPTAC-783
Peptide Molecular Mass
1,622.8245
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 16.3 11.1 8 16.7 13.6 7.8 23.3 17.6 11.2 15 15 15
y5 (1+) 22 9.4 7.1 27.1 11.8 8.5 34.9 15.1 11.1 15 15 15
y6 (1+) 22.9 10.8 11.8 24.8 12.9 9 33.8 16.8 14.8 15 15 15
y7 (1+) 12.8 7.2 7.6 15.2 7.3 6.5 19.9 10.3 10 15 15 15
y8 (1+) 15.5 11.6 9.5 22.3 13.1 11 27.2 17.5 14.5 15 15 15
b3 (1+) 44.5 41.7 16.3 30.3 48.7 18.4 53.8 64.1 24.6 15 15 15
sum 7.7 6.6 4.9 9.6 7.9 4.9 12.3 10.3 6.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-784 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT1
Peptide Sequence
QEEEEMDFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
113
Peptide End
121
CPTAC ID
CPTAC-784
Peptide Molecular Mass
1,211.4765
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 14.1 12.2 9.1 14.1 6.5 10.7 19.9 13.8 14 15 15 15
y5 (1+) 16.4 7.1 8.2 19 10.8 10.7 25.1 12.9 13.5 15 15 15
y6 (1+) 15.1 9.1 7.3 16.2 8.2 8.8 22.1 12.2 11.4 15 15 15
y7 (1+) 14.6 10 7.8 15.6 9.8 8.1 21.4 14 11.2 15 15 15
sum 10.1 4.1 4.5 10.2 5.2 6 14.4 6.6 7.5 15 15 15

Additional Resources and Comments