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GSK3B

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GSK3B N/A
Sequence Length (AA) Molecular Weight (Da)
420 46744
Protein Name
Glycogen synthase kinase-3 beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR
60 70 80 90 100
PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI
110 120 130 140 150
MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK
160 170 180 190 200
QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD
210 220 230 240 250
FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL
260 270 280 290 300
LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP
310 320 330 340 350
WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL
360 370 380 390 400
PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA
410 420
NTGDRGQTNN AASASASNST

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-804 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+80.0]FAESC[+57.0]KPVQQPSAFGSMK
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
9, 14
Peptide - Site of Modification
3, 8
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-804
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 25.6 12.5 8.5 33.1 17.8 10.8 41.8 21.8 13.7 15 15 15
y4 (1+) 28.8 15.5 9 19.8 15.7 13.3 34.9 22.1 16.1 15 15 15
y5 (1+) 21.1 11 10.5 18.8 9.3 10.6 28.3 14.4 14.9 15 15 15
y6 (1+) 23.2 12.1 6 21.6 15.9 8.9 31.7 20 10.7 15 15 15
y7 (1+) 24.9 7.7 18.6 26.9 8.4 15.7 36.7 11.4 24.3 15 15 15
y8 (1+) 8.9 6.6 2.9 9 7 5.6 12.7 9.6 6.3 15 15 15
y12 (1+) 9.9 9.1 9.2 13.6 8.1 10.9 16.8 12.2 14.3 15 15 15
sum 6.5 4.2 1.8 7 4.9 3.6 9.6 6.5 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
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