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Overview Data source: UniProt

Official Gene Symbol Other Aliases
GSK3B N/A
Sequence Length (AA) Molecular Weight (Da)
420 46744
Protein Name
Glycogen synthase kinase-3 beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR
60 70 80 90 100
PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI
110 120 130 140 150
MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK
160 170 180 190 200
QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD
210 220 230 240 250
FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL
260 270 280 290 300
LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP
310 320 330 340 350
WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL
360 370 380 390 400
PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA
410 420
NTGDRGQTNN AASASASNST

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5749 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Sequence
LLEYTPTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
320
Peptide End
328
CPTAC ID
CPTAC-5749
Peptide Molecular Mass
1,062.5710
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 15.1 8.7 4.5 23.5 23 10.9 27.9 24.6 11.8 15 15 15
y6 (1+) 25.2 8.9 4.4 31.5 20.1 11.6 40.3 22 12.4 15 15 15
y7 (1+) 28.5 7.4 3.9 18.6 23.1 11 34 24.3 11.7 15 15 15
sum 18.8 7.3 3.9 21.1 22.1 11.1 28.3 23.3 11.8 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5750 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TPPEAIALC[+57.021464]SR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
309
Peptide End
319
CPTAC ID
CPTAC-5750
Peptide Molecular Mass
1,213.6125
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 21.3 12.3 5.6 23.1 27 15.2 31.4 29.7 16.2 15 15 15
y5 (1+) 43.1 12.8 6.4 44.4 26.8 12.7 61.9 29.7 14.2 15 15 15
sum 24 10.9 4.5 23.9 26.4 13.9 33.9 28.6 14.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5790 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTSFAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-5790
Peptide Molecular Mass
2,287.0613
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y15 (2+) 12.1 4.2 9 16.7 16 12.4 20.6 16.5 15.3 16 15 17
y8 (1+) 14.1 4.1 9.2 19.5 15 12.4 24.1 15.6 15.4 16 15 17
sum 13 3.6 9.1 18.1 15.3 12.4 22.3 15.7 15.4 16 15 17


Additional Resources and Comments


Assay Details for CPTAC-5791 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+79.966331]FAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-5791
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.5 9.7 13.4 17.8 19.2 19.7 19.7 21.5 23.8 16 15 16
y15 (2+) 15.2 10.6 10.6 23.9 17.9 14.9 28.3 20.8 18.3 16 15 16
sum 9.1 9.9 11.7 19 18.6 16.8 21.1 21.1 20.5 16 15 16


Additional Resources and Comments


Assay Details for CPTAC-5751 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Sequence
VIGNGSFGVVYQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
61
Peptide End
74
CPTAC ID
CPTAC-5751
Peptide Molecular Mass
1,437.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 29.9 10.1 7.7 23.8 27.8 13.9 38.2 29.6 15.9 15 15 15
y5 (1+) 26.5 15.6 5.7 24.4 32.5 13.7 36 36.1 14.8 15 15 15
y7 (1+) 24.6 11.1 6.5 22.3 27.4 14.3 33.2 29.6 15.7 15 15 15
sum 24.2 11.5 6.5 20.5 29.1 13.8 31.7 31.3 15.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5556 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Sequence
LLEYTPTAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
320
Peptide End
328
CPTAC ID
non-CPTAC-5556
Peptide Molecular Mass
1,062.5710
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 16.1 5.9 4 23.8 23.3 10.5 28.7 24 11.2 14 14 14
y5 (1+) 23.8 7.5 4.1 26.7 23.9 11.6 35.8 25 12.3 14 14 14
y6 (1+) 21.2 6.3 3.3 29.3 20.5 11 36.2 21.4 11.5 14 14 14
y7 (1+) 21.4 4.1 3.2 20.7 23.6 11 29.8 24 11.5 14 14 14
sum 18.2 4.1 3.2 22.8 22.7 10.9 29.2 23.1 11.4 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5403 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Sequence
QTLPVIYVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
151
Peptide End
159
CPTAC ID
non-CPTAC-5403
Peptide Molecular Mass
1,059.6328
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.2 2.8 2.6 6.1 3 3.7 7.4 4.1 4.5 15 15 15
y7 (1+) 9.4 6.3 3.8 6.3 7.2 4.1 11.3 9.6 5.6 15 15 15
y3 (1+) 9 4.1 3.4 11.5 4.5 3.2 14.6 6.1 4.7 15 15 15
y5 (1+) 18.1 6.8 5.3 20.9 6.4 4.2 27.6 9.3 6.8 15 15 15
sum 4.1 2 2.1 6.2 2.7 2.9 7.4 3.4 3.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5404 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TPPEAIALC[+57.021464]SR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
318
Peptide - Site of Modification
9
Peptide Start
309
Peptide End
319
CPTAC ID
non-CPTAC-5404
Peptide Molecular Mass
1,213.6125
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 2.4 1.7 1.8 3.3 2.1 1.9 4.1 2.7 2.6 15 15 15
y5 (1+) 6 2.5 1.4 5.5 3.5 3.4 8.1 4.3 3.7 15 15 15
y6 (1+) 9.1 2.4 2.3 9.9 2.9 4.6 13.4 3.8 5.1 15 15 15
y7 (1+) 5.7 2.2 2.7 6 2.5 4.4 8.3 3.3 5.2 15 15 15
y8 (1+) 8.2 3 3.5 7.5 4 3.3 11.1 5 4.8 15 15 15
sum 2.2 1.2 1.4 2.5 1.6 2.7 3.3 2 3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5401 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTSFAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
15
Peptide - Site of Modification
8
Peptide Start
7
Peptide End
27
CPTAC ID
non-CPTAC-5401
Peptide Molecular Mass
2,287.0613
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16 (2+) 7.5 4.9 3.3 9.6 5.7 4.9 12.2 7.5 5.9 15 15 15
y17 (2+) 11 5.1 4.2 11.4 5.9 4.5 15.8 7.8 6.2 15 15 15
y19 (2+) 10.4 4.1 3 12.8 7.3 3.7 16.5 8.4 4.8 15 15 15
y8 (1+) 7 2.6 2.9 8.1 6.3 3.4 10.7 6.8 4.5 15 15 15
y12 (1+) 20 10.5 5.6 16.6 6.7 6.6 26 12.5 8.7 15 15 15
sum 3.3 2.1 1.8 5.1 3.8 2.9 6.1 4.3 3.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5705 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+79.966331]FAESC[+57.021464]KPVQQPSAFGSMK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
7
Peptide End
27
CPTAC ID
non-CPTAC-5705
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N K
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 12.7 4.9 5.7 13 6.8 7.5 18.2 8.4 9.4 15 15 15
y8 (1+) 3.4 3.7 2.3 4.5 4 3.8 5.6 5.4 4.4 15 15 15
y17 (2+) 8.6 10.5 5.4 10.5 11.6 6.1 13.6 15.6 8.1 15 15 15
y16 (2+) 5.9 5.2 4.4 6.8 8.1 6.4 9 9.6 7.8 15 15 15
y15 (2+) 10.6 7.9 4.9 10.7 8.3 5.3 15.1 11.5 7.2 15 15 15
sum 3.6 2.8 1.8 4 3.2 2.4 5.4 4.3 3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5554 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Sequence
VIGNGSFGVVYQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
61
Peptide End
74
CPTAC ID
non-CPTAC-5554
Peptide Molecular Mass
1,437.7616
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Immuno-MRM
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 28.4 9.3 7.3 22.9 28.5 13.2 36.5 30 15.1 14 14 14
y5 (1+) 29 12.7 5.9 25.8 33.4 13.5 38.8 35.7 14.7 14 14 14
y7 (1+) 26.5 9.6 5.1 23.5 28.8 12.6 35.4 30.4 13.6 14 14 14
y8 (1+) 29.1 10.8 3.8 30.6 33.8 13.9 42.2 35.5 14.4 14 14 14
y12 (1+) 23.7 8.2 6.9 30.9 27.3 14.2 38.9 28.5 15.8 14 14 14
sum 25.6 9.3 5.8 24.5 29.8 13.3 35.4 31.2 14.5 14 14 14


Additional Resources and Comments


Assay Details for CPTAC-804 Collapse assay details

Data source: Panorama

Official Gene Symbol
GSK3B
Peptide Modified Sequence
TTS[+80.0]FAESC[+57.0]KPVQQPSAFGSMK
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
9, 14
Peptide - Site of Modification
3, 8
Peptide Start
7
Peptide End
27
CPTAC ID
CPTAC-804
Peptide Molecular Mass
2,367.0276
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 25.6 12.5 8.5 33.1 17.8 10.8 41.8 21.8 13.7 15 15 15
y4 (1+) 28.8 15.5 9 19.8 15.7 13.3 34.9 22.1 16.1 15 15 15
y5 (1+) 21.1 11 10.5 18.8 9.3 10.6 28.3 14.4 14.9 15 15 15
y6 (1+) 23.2 12.1 6 21.6 15.9 8.9 31.7 20 10.7 15 15 15
y7 (1+) 24.9 7.7 18.6 26.9 8.4 15.7 36.7 11.4 24.3 15 15 15
y8 (1+) 8.9 6.6 2.9 9 7 5.6 12.7 9.6 6.3 15 15 15
y12 (1+) 9.9 9.1 9.2 13.6 8.1 10.9 16.8 12.2 14.3 15 15 15
sum 6.5 4.2 1.8 7 4.9 3.6 9.6 6.5 4 15 15 15


Additional Resources and Comments