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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK17 PCTAIRE2, PCTK2
Sequence Length (AA) Molecular Weight (Da)
523 59582
Protein Name
Cyclin-dependent kinase 17
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKKFKRRLSL TLRGSQTIDE SLSELAEQMT IEENSSKDNE PIVKNGRPPT
60 70 80 90 100
SHSMHSFLHQ YTGSFKKPPL RRPHSVIGGS LGSFMAMPRN GSRLDIVHEN
110 120 130 140 150
LKMGSDGESD QASGTSSDEV QSPTGVCLRN RIHRRISMED LNKRLSLPAD
160 170 180 190 200
IRIPDGYLEK LQINSPPFDQ PMSRRSRRAS LSEIGFGKME TYIKLEKLGE
210 220 230 240 250
GTYATVYKGR SKLTENLVAL KEIRLEHEEG APCTAIREVS LLKDLKHANI
260 270 280 290 300
VTLHDIVHTD KSLTLVFEYL DKDLKQYMDD CGNIMSMHNV KLFLYQILRG
310 320 330 340 350
LAYCHRRKVL HRDLKPQNLL INEKGELKLA DFGLARAKSV PTKTYSNEVV
360 370 380 390 400
TLWYRPPDVL LGSSEYSTQI DMWGVGCIFF EMASGRPLFP GSTVEDELHL
410 420 430 440 450
IFRLLGTPSQ ETWPGISSNE EFKNYNFPKY KPQPLINHAP RLDSEGIELI
460 470 480 490 500
TKFLQYESKK RVSAEEAMKH VYFRSLGPRI HALPESVSIF SLKEIQLQKD
510 520 523
PGFRNSSYPE TGHGKNRRQS MLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5667 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK17
Peptide Sequence
ASLSEIGFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
179
Peptide End
188
CPTAC ID
non-CPTAC-5667
Peptide Molecular Mass
1,007.5288
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 2.5 2 5 3 2.1 6 3.9 2.9 7.8 15 15 15
y5 (1+) 3.2 2.7 5.2 4.3 3.6 5.6 5.4 4.5 7.6 15 15 15
y4 (1+) 2.6 3 4.9 3.1 2.9 5.1 4 4.2 7.1 15 15 15
sum 2.3 1.9 2.5 2.9 2.1 4.4 3.7 2.8 5.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2937 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK17
Peptide Sequence
FLQYESK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
453
Peptide End
459
CPTAC ID
non-CPTAC-2937
Peptide Molecular Mass
913.4545
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 6.3 3.2 3.1 6.1 4.5 3.3 8.8 5.5 4.5 15 15 15
y5 (1+) 5 3.9 3 6.5 4.7 3.2 8.2 6.1 4.4 15 15 15
y3 (1+) 4.7 4.5 2.4 5.2 4.2 3.5 7 6.2 4.2 15 15 15
sum 3.2 3.5 2.5 3.9 3.9 2.8 5 5.2 3.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2938 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK17
Peptide Sequence
IHALPESVSIFSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
480
Peptide End
493
CPTAC ID
non-CPTAC-2938
Peptide Molecular Mass
1,539.8661
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.8 8.7 12.1 13.8 13.2 12.7 17.5 15.8 17.5 15 15 15
b3 (1+) 9.5 9.2 12.1 8.9 14 12 13 16.8 17 15 15 15
b4 (1+) 10.4 9.7 11.7 12.7 14.1 12.4 16.4 17.1 17 15 15 15
sum 9.9 9.1 11.9 11.3 13.7 12.3 15 16.4 17.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5666 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK17
Peptide Sequence
LDSEGIELITK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
442
Peptide End
452
CPTAC ID
non-CPTAC-5666
Peptide Molecular Mass
1,216.6551
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.3 2.2 5.4 6 2.9 5.6 6.8 3.6 7.8 15 15 15
y9 (1+) 4.3 3.8 7.5 6 4.1 8 7.4 5.6 11 15 15 15
y5 (1+) 3.9 3.6 11.3 5.9 4.4 14.2 7.1 5.7 18.1 15 15 15
sum 3.2 2.2 3.8 5.4 2.7 4 6.3 3.5 5.5 15 15 15


Additional Resources and Comments