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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK10 N/A
Sequence Length (AA) Molecular Weight (Da)
360 41038
Protein Name
Cyclin-dependent kinase 10
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEPDLECEQ IRLKCIRKEG FFTVPPEHRL GRCRSVKEFE KLNRIGEGTY
60 70 80 90 100
GIVYRARDTQ TDEIVALKKV RMDKEKDGIP ISSLREITLL LRLRHPNIVE
110 120 130 140 150
LKEVVVGNHL ESIFLVMGYC EQDLASLLEN MPTPFSEAQV KCIVLQVLRG
160 170 180 190 200
LQYLHRNFII HRDLKVSNLL MTDKGCVKTA DFGLARAYGV PVKPMTPKVV
210 220 230 240 250
TLWYRAPELL LGTTTQTTSI DMWAVGCILA ELLAHRPLLP GTSEIHQIDL
260 270 280 290 300
IVQLLGTPSE NIWPGFSKLP LVGQYSLRKQ PYNNLKHKFP WLSEAGLRLL
310 320 330 340 350
HFLFMYDPKK RATAGDCLES SYFKEKPLPC EPELMPTFPH HRNKRAAPAT
360
SEGQSKRCKP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5662 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK10
Peptide Sequence
DGIPISSLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
77
Peptide End
85
CPTAC ID
non-CPTAC-5662
Peptide Molecular Mass
956.5291
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 2.5 2.1 3.1 2.5 2.7 2.9 3.5 3.4 4.2 15 15 15
y5 (1+) 2.4 1.9 3.2 3.7 3.6 6.1 4.4 4.1 6.9 15 15 15
y4 (1+) 2.5 1.4 6.2 3.7 2.1 5.5 4.5 2.5 8.3 15 15 15
sum 2 1.3 2.9 2.5 2.3 2.7 3.2 2.6 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5663 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK10
Peptide Sequence
DTQTDEIVALK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
58
Peptide End
68
CPTAC ID
non-CPTAC-5663
Peptide Molecular Mass
1,231.6296
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 3.4 2.2 2.6 6.2 3.5 4.7 7.1 4.1 5.4 15 15 15
y7 (1+) 3.2 4.4 4.4 3.1 4.1 5.1 4.5 6 6.7 15 15 15
y4 (1+) 4 2.9 5.4 4.6 4.4 6.2 6.1 5.3 8.2 15 15 15
sum 3 2.3 2.9 4.6 3.1 3.3 5.5 3.9 4.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2943 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK10
Peptide Sequence
FPWLSEAGLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
289
Peptide End
298
CPTAC ID
non-CPTAC-2943
Peptide Molecular Mass
1,174.6135
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3.8 6.6 8 9.8 9.7 10 10.5 11.7 12.8 15 15 15
y7 (1+) 4.8 6.4 8 9.6 10.2 10.8 10.7 12 13.4 15 15 15
y8 (1+) 3.1 6.7 8.5 10.5 10.1 10.6 10.9 12.1 13.6 15 15 15
sum 3.7 6.5 8.1 9.9 10 10.5 10.6 11.9 13.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2944 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK10
Peptide Sequence
TADFGLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
186
CPTAC ID
non-CPTAC-2944
Peptide Molecular Mass
849.4345
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.7 4.7 3.3 7.2 3.4 3.6 9.8 5.8 4.9 15 15 15
y5 (1+) 7.2 3.7 3.4 6.4 2.7 3.6 9.6 4.6 5 15 15 15
y4 (1+) 8.3 3.1 3.1 8.4 2.6 3.2 11.8 4 4.5 15 15 15
sum 7 3.6 3.1 6.7 2.6 3.2 9.7 4.4 4.5 15 15 15


Additional Resources and Comments