CLK4, CPTAC-2952 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CLK4	N/A

Sequence Length (AA)	Molecular Weight (Da)
481	57492

Protein Name
Dual specificity protein kinase CLK4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRHSKRTHCP	DWDSRESWGH	ESYRGSHKRK	RRSHSSTQEN	RHCKPHHQFK
60	70	80	90	100
ESDCHYLEAR	SLNERDYRDR	RYVDEYRNDY	CEGYVPRHYH	RDIESGYRIH
110	120	130	140	150
CSKSSVRSRR	SSPKRKRNRH	CSSHQSRSKS	HRRKRSRSIE	DDEEGHLICQ
160	170	180	190	200
SGDVLRARYE	IVDTLGEGAF	GKVVECIDHG	MDGMHVAVKI	VKNVGRYREA
210	220	230	240	250
ARSEIQVLEH	LNSTDPNSVF	RCVQMLEWFD	HHGHVCIVFE	LLGLSTYDFI
260	270	280	290	300
KENSFLPFQI	DHIRQMAYQI	CQSINFLHHN	KLTHTDLKPE	NILFVKSDYV
310	320	330	340	350
VKYNSKMKRD	ERTLKNTDIK	VVDFGSATYD	DEHHSTLVST	RHYRAPEVIL
360	370	380	390	400
ALGWSQPCDV	WSIGCILIEY	YLGFTVFQTH	DSKEHLAMME	RILGPIPQHM
410	420	430	440	450
IQKTRKRKYF	HHNQLDWDEH	SSAGRYVRRR	CKPLKEFMLC	HDEEHEKLFD
460	470	480	481
LVRRMLEYDP	TQRITLDEAL	QHPFFDLLKK	K

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5638 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK4
Peptide Sequence: DIESGYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 92
Peptide End: 98

CPTAC ID: non-CPTAC-5638
Peptide Molecular Mass: 838.3821
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	1.2	1.7	9	1.5	2.5	9.7	1.9	3	13.2	15	15	15
y4 (1+)	1.3	1.5	4.1	2.8	2.5	4	3.1	2.9	5.7	15	15	15
y5 (1+)	1.2	1.5	4.3	4.3	4.2	3.3	4.5	4.5	5.4	15	15	15
sum	0.7	1.1	2.8	3.3	3.1	2.2	3.4	3.3	3.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5639 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK4
Peptide Sequence: ESWGHESYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 16
Peptide End: 24

CPTAC ID: non-CPTAC-5639
Peptide Molecular Mass: 1,149.4839
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend