DYRK1B, CPTAC-2984 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
DYRK1B	MIRK

Sequence Length (AA)	Molecular Weight (Da)
629	69198

Protein Name
Dual specificity tyrosine-phosphorylation-regulated kinase 1B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAVPPGHGPF	SGFPGPQEHT	QVLPDVRLLP	RRLPLAFRDA	TSAPLRKLSV
60	70	80	90	100
DLIKTYKHIN	EVYYAKKKRR	AQQAPPQDSS	NKKEKKVLNH	GYDDDNHDYI
110	120	130	140	150
VRSGERWLER	YEIDSLIGKG	SFGQVVKAYD	HQTQELVAIK	IIKNKKAFLN
160	170	180	190	200
QAQIELRLLE	LMNQHDTEMK	YYIVHLKRHF	MFRNHLCLVF	ELLSYNLYDL
210	220	230	240	250
LRNTHFRGVS	LNLTRKLAQQ	LCTALLFLAT	PELSIIHCDL	KPENILLCNP
260	270	280	290	300
KRSAIKIVDF	GSSCQLGQRI	YQYIQSRFYR	SPEVLLGTPY	DLAIDMWSLG
310	320	330	340	350
CILVEMHTGE	PLFSGSNEVD	QMNRIVEVLG	IPPAAMLDQA	PKARKYFERL
360	370	380	390	400
PGGGWTLRRT	KELRKDYQGP	GTRRLQEVLG	VQTGGPGGRR	AGEPGHSPAD
410	420	430	440	450
YLRFQDLVLR	MLEYEPAARI	SPLGALQHGF	FRRTADEATN	TGPAGSSAST
460	470	480	490	500
SPAPLDTCPS	SSTASSISSS	GGSSGSSSDN	RTYRYSNRYC	GGPGPPITDC
510	520	530	540	550
EMNSPQVPPS	QPLRPWAGGD	VPHKTHQAPA	SASSLPGTGA	QLPPQPRYLG
560	570	580	590	600
RPPSPTSPPP	PELMDVSLVG	GPADCSPPHP	APAPQHPAAS	ALRTRMTGGR
610	620	629
PPLPPPDDPA	TLGPHLGLRG	VPQSTAASS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2984 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK1B
Peptide Sequence: ISPLGALQHGFFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 420
Peptide End: 432

CPTAC ID: non-CPTAC-2984
Peptide Molecular Mass: 1,441.7830
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	8.1	7.2	5.7	11.2	10.9	6.2	13.8	13.1	8.4	15	15	15
y9 (1+)	8.4	6.6	6.4	13.1	11.6	7.2	15.6	13.3	9.6	15	15	15
y4 (1+)	7.9	5.5	6	10.3	9.3	5.9	13	10.8	8.4	15	15	15
sum	8	6.4	5.8	11.2	10.4	6.1	13.8	12.2	8.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5630 Collapse assay details

Data source: Panorama

Official Gene Symbol: DYRK1B
Peptide Sequence: LQEVLGVQTGGPGGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 375
Peptide End: 389

CPTAC ID: non-CPTAC-5630
Peptide Molecular Mass: 1,466.7841
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend