PHKG2, CPTAC-3114 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PHKG2	N/A

Sequence Length (AA)	Molecular Weight (Da)
406	46442

Protein Name
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTLDVGPEDE	LPDWAAAKEF	YQKYDPKDVI	GRGVSSVVRR	CVHRATGHEF
60	70	80	90	100
AVKIMEVTAE	RLSPEQLEEV	REATRRETHI	LRQVAGHPHI	ITLIDSYESS
110	120	130	140	150
SFMFLVFDLM	RKGELFDYLT	EKVALSEKET	RSIMRSLLEA	VSFLHANNIV
160	170	180	190	200
HRDLKPENIL	LDDNMQIRLS	DFGFSCHLEP	GEKLRELCGT	PGYLAPEILK
210	220	230	240	250
CSMDETHPGY	GKEVDLWACG	VILFTLLAGS	PPFWHRRQIL	MLRMIMEGQY
260	270	280	290	300
QFSSPEWDDR	SSTVKDLISR	LLQVDPEARL	TAEQALQHPF	FERCEGSQPW
310	320	330	340	350
NLTPRQRFRV	AVWTVLAAGR	VALSTHRVRP	LTKNALLRDP	YALRSVRHLI
360	370	380	390	400
DNCAFRLYGH	WVKKGEQQNR	AALFQHRPPG	PFPIMGPEEE	GDSAAITEDE
406
AVLVLG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3114 Collapse assay details

Data source: Panorama

Official Gene Symbol: PHKG2
Peptide Modified Sequence: ELC[+57.0]GTPGYLAPEILK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 189
Peptide - Site of Modification: 3
Peptide Start: 186
Peptide End: 200

CPTAC ID: non-CPTAC-3114
Peptide Molecular Mass: 1,659.8542
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	5.4	7.2	6.3	5.2	10.1	10.9	7.5	12.4	12.6	15	15	15
y6 (1+)	5.1	6.1	6.6	5.7	7.8	9.4	7.6	9.9	11.5	15	15	15
y5 (1+)	4.4	6.4	5.6	4.6	8.8	9	6.4	10.9	10.6	15	15	15
sum	4.5	6.7	6	4.9	9.3	10	6.7	11.5	11.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5687 Collapse assay details

Data source: Panorama

Official Gene Symbol: PHKG2
Peptide Sequence: LLQVDPEAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 271
Peptide End: 279

CPTAC ID: non-CPTAC-5687
Peptide Molecular Mass: 1,039.5662
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend