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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP3K14

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K14 NIK
Sequence Length (AA) Molecular Weight (Da)
947 104042
Protein Name
Mitogen-activated protein kinase kinase kinase 14
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAVMEMACPG APGSAVGQQK ELPKAKEKTP PLGKKQSSVY KLEAVEKSPV
60 70 80 90 100
FCGKWEILND VITKGTAKEG SEAGPAAISI IAQAECENSQ EFSPTFSERI
110 120 130 140 150
FIAGSKQYSQ SESLDQIPNN VAHATEGKMA RVCWKGKRRS KARKKRKKKS
160 170 180 190 200
SKSLAHAGVA LAKPLPRTPE QESCTIPVQE DESPLGAPYV RNTPQFTKPL
210 220 230 240 250
KEPGLGQLCF KQLGEGLRPA LPRSELHKLI SPLQCLNHVW KLHHPQDGGP
260 270 280 290 300
LPLPTHPFPY SRLPHPFPFH PLQPWKPHPL ESFLGKLACV DSQKPLPDPH
310 320 330 340 350
LSKLACVDSP KPLPGPHLEP SCLSRGAHEK FSVEEYLVHA LQGSVSSGQA
360 370 380 390 400
HSLTSLAKTW AARGSRSREP SPKTEDNEGV LLTEKLKPVD YEYREEVHWA
410 420 430 440 450
THQLRLGRGS FGEVHRMEDK QTGFQCAVKK VRLEVFRAEE LMACAGLTSP
460 470 480 490 500
RIVPLYGAVR EGPWVNIFME LLEGGSLGQL VKEQGCLPED RALYYLGQAL
510 520 530 540 550
EGLEYLHSRR ILHGDVKADN VLLSSDGSHA ALCDFGHAVC LQPDGLGKSL
560 570 580 590 600
LTGDYIPGTE THMAPEVVLG RSCDAKVDVW SSCCMMLHML NGCHPWTQFF
610 620 630 640 650
RGPLCLKIAS EPPPVREIPP SCAPLTAQAI QEGLRKEPIH RVSAAELGGK
660 670 680 690 700
VNRALQQVGG LKSPWRGEYK EPRHPPPNQA NYHQTLHAQP RELSPRAPGP
710 720 730 740 750
RPAEETTGRA PKLQPPLPPE PPEPNKSPPL TLSKEESGMW EPLPLSSLEP
760 770 780 790 800
APARNPSSPE RKATVPEQEL QQLEIELFLN SLSQPFSLEE QEQILSCLSI
810 820 830 840 850
DSLSLSDDSE KNPSKASQSS RDTLSSGVHS WSSQAEARSS SWNMVLARGR
860 870 880 890 900
PTDTPSYFNG VKVQIQSLNG EHLHIREFHR VKVGDIATGI SSQIPAAAFS
910 920 930 940 947
LVTKDGQPVR YDMEVPDSGI DLQCTLAPDG SFAWSWRVKH GQLENRP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5641 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K14
Peptide Sequence
TEDNEGVLLTEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
374
Peptide End
385
CPTAC ID
non-CPTAC-5641
Peptide Molecular Mass
1,346.6565
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 1.8 1.7 4 3.9 2.4 4.2 4.3 2.9 5.8 15 15 15
y5 (1+) 2.1 2.3 4.5 3.1 2.1 5.1 3.7 3.1 6.8 15 15 15
y7 (1+) 1.8 1.2 3.7 2.7 1.5 4.5 3.2 1.9 5.8 15 15 15
sum 1.5 1.5 2.2 2.7 1.7 2.6 3.1 2.3 3.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments