LMTK3, CPTAC-5642 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
LMTK3	KIAA1883, TYKLM3

Sequence Length (AA)	Molecular Weight (Da)
1460	153661

Protein Name
Serine/threonine-protein kinase LMTK3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPAPGALILL	AAVSASGCLA	SPAHPDGFAL	GRAPLAPPYA	VVLISCSGLL
60	70	80	90	100
AFIFLLLTCL	CCKRGDVGFK	EFENPEGEDC	SGEYTPPAEE	TSSSQSLPDV
110	120	130	140	150
YILPLAEVSL	PMPAPQPSHS	DMTTPLGLSR	QHLSYLQEIG	SGWFGKVILG
160	170	180	190	200
EIFSDYTPAQ	VVVKELRASA	GPLEQRKFIS	EAQPYRSLQH	PNVLQCLGLC
210	220	230	240	250
VETLPFLLIM	EFCQLGDLKR	YLRAQRPPEG	LSPELPPRDL	RTLQRMGLEI
260	270	280	290	300
ARGLAHLHSH	NYVHSDLALR	NCLLTSDLTV	RIGDYGLAHS	NYKEDYYLTP
310	320	330	340	350
ERLWIPLRWA	APELLGELHG	TFMVVDQSRE	SNIWSLGVTL	WELFEFGAQP
360	370	380	390	400
YRHLSDEEVL	AFVVRQQHVK	LARPRLKLPY	ADYWYDILQS	CWRPPAQRPS
410	420	430	440	450
ASDLQLQLTY	LLSERPPRPP	PPPPPPRDGP	FPWPWPPAHS	APRPGTLSSP
460	470	480	490	500
FPLLDGFPGA	DPDDVLTVTE	SSRGLNLECL	WEKARRGAGR	GGGAPAWQPA
510	520	530	540	550
SAPPAPHANP	SNPFYEALST	PSVLPVISAR	SPSVSSEYYI	RLEEHGSPPE
560	570	580	590	600
PLFPNDWDPL	DPGVPAPQAP	QAPSEVPQLV	SETWASPLFP	APRPFPAQSS
610	620	630	640	650
ASGSFLLSGW	DPEGRGAGET	LAGDPAEVLG	ERGTAPWVEE	EEEEEEGSSP
660	670	680	690	700
GEDSSSLGGG	PSRRGPLPCP	LCSREGACSC	LPLERGDAVA	GWGGHPALGC
710	720	730	740	750
PHPPEDDSSL	RAERGSLADL	PMAPPASAPP	EFLDPLMGAA	APQYPGRGPP
760	770	780	790	800
PAPPPPPPPP	RAPADPAASP	DPPSAVASPG	SGLSSPGPKP	GDSGYETETP
810	820	830	840	850
FSPEGAFPGG	GAAEEEGVPR	PRAPPEPPDP	GAPRPPPDPG	PLPLPGPREK
860	870	880	890	900
PTFVVQVSTE	QLLMSLREDV	TRNLLGEKGA	TARETGPRKA	GRGPGNREKV
910	920	930	940	950
PGLNRDPTVL	GNGKQAPSLS	LPVNGVTVLE	NGDQRAPGIE	EKAAENGALG
960	970	980	990	1000
SPEREEKVLE	NGELTPPRRE	EKALENGELR	SPEAGEKVLV	NGGLTPPKSE
1010	1020	1030	1040	1050
DKVSENGGLR	FPRNTERPPE	TGPWRAPGPW	EKTPESWGPA	PTIGEPAPET
1060	1070	1080	1090	1100
SLERAPAPSA	VVSSRNGGET	APGPLGPAPK	NGTLEPGTER	RAPETGGAPR
1110	1120	1130	1140	1150
APGAGRLDLG	SGGRAPVGTG	TAPGGGPGSG	VDAKAGWVDN	TRPQPPPPPL
1160	1170	1180	1190	1200
PPPPEAQPRR	LEPAPPRARP	EVAPEGEPGA	PDSRAGGDTA	LSGDGDPPKP
1210	1220	1230	1240	1250
ERKGPEMPRL	FLDLGPPQGN	SEQIKARLSR	LSLALPPLTL	TPFPGPGPRR
1260	1270	1280	1290	1300
PPWEGADAGA	AGGEAGGAGA	PGPAEEDGED	EDEDEEEDEE	AAAPGAAAGP
1310	1320	1330	1340	1350
RGPGRARAAP	VPVVVSSADA	DAARPLRGLL	KSPRGADEPE	DSELERKRKM
1360	1370	1380	1390	1400
VSFHGDVTVY	LFDQETPTNE	LSVQAPPEGD	TDPSTPPAPP	TPPHPATPGD
1410	1420	1430	1440	1450
GFPSNDSGFG	GSFEWAEDFP	LLPPPGPPLC	FSRFSVSPAL	ETPGPPARAP
1460
DARPAGPVEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-5643 Collapse assay details

Data source: Panorama

Official Gene Symbol: LMTK3
Peptide Sequence: GAGETLAGDPAEVLGER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 616
Peptide End: 632

CPTAC ID: non-CPTAC-5643
Peptide Molecular Mass: 1,640.8006
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	1.7	1.9	3.6	2.2	2.1	5.2	2.8	2.8	6.3	15	15	15
y10 (1+)	2.3	3.3	4.6	3.5	3.3	5.9	4.2	4.7	7.5	15	15	15
y11 (1+)	2.3	2.8	4.6	3.3	2.4	7.3	4	3.7	8.6	15	15	15
sum	1.8	2	3.3	2.3	1.7	5.2	2.9	2.6	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5642 Collapse assay details

Data source: Panorama

Official Gene Symbol: LMTK3
Peptide Sequence: LFLDLGPPQGNSEQIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1210
Peptide End: 1225

CPTAC ID: non-CPTAC-5642
Peptide Molecular Mass: 1,754.9203
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend