PRAG1, CPTAC-5651 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRAG1	SGK223

Sequence Length (AA)	Molecular Weight (Da)
1406	149624

Protein Name
Inactive tyrosine-protein kinase PRAG1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MHQTLCLNPE	SLKMSACSDF	VEHIWKPGSC	KNCFCLRSDH	QLVAGPPQPR
60	70	80	90	100
AGSLPPPPRL	PPRPENCRLE	DEGVNSSPYS	KPTIAVKPTM	MSSEASDVWT
110	120	130	140	150
EANLSAEVSQ	VIWRRAPGKL	PLPKQEDAPV	VYLGSFRGVQ	KPAGPSTSPD
160	170	180	190	200
GNSRCPPAYT	MVGLHNLEPR	GERNIAFHPV	SFPEEKAVHK	EKPSFPYQDR
210	220	230	240	250
PSTQESFRQK	LAAFAGTTSG	CHQGPGPLRE	SLPSEDDSDQ	RCSPSGDSEG
260	270	280	290	300
GEYCSILDCC	PGSPVAKAAS	QTAGSRGRHG	GRDCSPTCWE	QGKCSGPAEQ
310	320	330	340	350
EKRGPSFPKE	CCSQGPTAHP	SCLGPKKLSL	TSEAAISSDG	LSCGSGSGSG
360	370	380	390	400
SGASSPFVPH	LESDYCSLMK	EPAPEKQQDP	GCPGVTPSRC	LGLTGEPQPP
410	420	430	440	450
AHPREATQPE	PIYAESTKRK	KAAPVPSKSQ	AKIEHAAAAQ	GQGQVCTGNA
460	470	480	490	500
WAQKAASGWG	RDSPDPTPQV	SATITVMAAH	PEEDHRTIYL	SSPDSAVGVQ
510	520	530	540	550
WPRGPVSQNS	EVGEEETSAG	QGLSSRESHA	HSASESKPKE	RPAIPPKLSK
560	570	580	590	600
SSPVGSPVSP	SAGGPPVSPL	ADLSDGSSGG	SSIGPQPPSQ	GPADPAPSCR
610	620	630	640	650
TNGVAISDPS	RCPQPAASSA	SEQRRPRFQA	GTWSRQCRIE	EEEEVEQELL
660	670	680	690	700
SHSWGRETKN	GPTDHSNSTT	WHRLHPTDGS	SGQNSKVGTG	MSKSASFAFE
710	720	730	740	750
FPKDRSGIET	FSPPPPPPKS	RHLLKMNKSS	SDLEKVSQGS	AESLSPSFRG
760	770	780	790	800
VHVSFTTGST	DSLASDSRTC	SDGGPSSELA	HSPTNSGKKL	FAPVPFPSGS
810	820	830	840	850
TEDVSPSGPQ	QPPPLPQKKI	VSRAASSPDG	FFWTQGSPKP	GTASPKLNLS
860	870	880	890	900
HSETNVHDES	HFSYSLSPGN	RHHPVFSSSD	PLEKAFKGSG	HWLPAAGLAG
910	920	930	940	950
NRGGCGSPGL	QCKGAPSASS	SQLSVSSQAS	TGSTQLQLHG	LLSNISSKEG
960	970	980	990	1000
TYAKLGGLYT	QSLARLVAKC	EDLFMGGQKK	ELHFNENNWS	LFKLTCNKPC
1010	1020	1030	1040	1050
CDSGDAIYYC	ATCSEDPGST	YAVKICKAPE	PKTVSYCSPS	VPVHFNIQQD
1060	1070	1080	1090	1100
CGHFVASVPS	SMLSSPDAPK	DPVPALPTHP	PAQEQDCVVV	ITREVPHQTA
1110	1120	1130	1140	1150
SDFVRDSAAS	HQAEPEAYER	RVCFLLLQLC	NGLEHLKEHG	IIHRDLCLEN
1160	1170	1180	1190	1200
LLLVHCTLQA	GPGPAPAPAP	APAPAAAAPP	CSSAAPPAGG	TLSPAAGPAS
1210	1220	1230	1240	1250
PEGPREKQLP	RLIISNFLKA	KQKPGGTPNL	QQKKSQARLA	PEIVSASQYR
1260	1270	1280	1290	1300
KFDEFQTGIL	IYELLHQPNP	FEVRAQLRER	DYRQEDLPPL	PALSLYSPGL
1310	1320	1330	1340	1350
QQLAHLLLEA	DPIKRIRIGE	AKRVLQCLLW	GPRRELVQQP	GTSEEALCGT
1360	1370	1380	1390	1400
LHNWIDMKRA	LMMMKFAEKA	VDRRRGVELE	DWLCCQYLAS	AEPGALLQSL
1406
KLLQLL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-5650 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRAG1
Peptide Sequence: LAPEIVSASQYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1239
Peptide End: 1250

CPTAC ID: non-CPTAC-5650
Peptide Molecular Mass: 1,332.7038
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	1.8	1.6	4.5	2	1.7	4	2.7	2.3	6	15	15	15
y7 (1+)	1	1.6	3.2	1.3	1.8	3.9	1.6	2.4	5	15	15	15
y6 (1+)	1.4	2.3	2	2.7	3.3	2.6	3	4	3.3	15	15	15
sum	0.9	1.5	2.5	1.7	2	2.7	1.9	2.5	3.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-5651 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRAG1
Peptide Sequence: LGGLYTQSLAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 955
Peptide End: 965

CPTAC ID: non-CPTAC-5651
Peptide Molecular Mass: 1,177.6455
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend