VRK2, CPTAC-5652 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
VRK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
508	58141

Protein Name
Serine/threonine-protein kinase VRK2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPPKRNEKYK	LPIPFPEGKV	LDDMEGNQWV	LGKKIGSGGF	GLIYLAFPTN
60	70	80	90	100
KPEKDARHVV	KVEYQENGPL	FSELKFYQRV	AKKDCIKKWI	ERKQLDYLGI
110	120	130	140	150
PLFYGSGLTE	FKGRSYRFMV	MERLGIDLQK	ISGQNGTFKK	STVLQLGIRM
160	170	180	190	200
LDVLEYIHEN	EYVHGDIKAA	NLLLGYKNPD	QVYLADYGLS	YRYCPNGNHK
210	220	230	240	250
QYQENPRKGH	NGTIEFTSLD	AHKGVALSRR	SDVEILGYCM	LRWLCGKLPW
260	270	280	290	300
EQNLKDPVAV	QTAKTNLLDE	LPQSVLKWAP	SGSSCCEIAQ	FLVCAHSLAY
310	320	330	340	350
DEKPNYQALK	KILNPHGIPL	GPLDFSTKGQ	SINVHTPNSQ	KVDSQKAATK
360	370	380	390	400
QVNKAHNRLI	EKKVHSERSA	ESCATWKVQK	EEKLIGLMNN	EAAQESTRRR
410	420	430	440	450
QKYQESQEPL	NEVNSFPQKI	SYTQFPNSFY	EPHQDFTSPD	IFKKSRSPSW
460	470	480	490	500
YKYTSTVSTG	ITDLESSTGL	WPTISQFTLS	EETNADVYYY	RIIIPVLLML
508
VFLALFFL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5652 Collapse assay details

Data source: Panorama

Official Gene Symbol: VRK2
Peptide Sequence: AANLLLGYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 169
Peptide End: 177

CPTAC ID: non-CPTAC-5652
Peptide Molecular Mass: 961.5597
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	3.1	2.9	6.9	2.9	2.9	7.1	4.2	4.1	9.9	15	15	15
y4 (1+)	2.9	2.9	7	3.8	4.7	8.1	4.8	5.5	10.7	15	15	15
y3 (1+)	2.6	3.5	3.8	2.9	4.9	4	3.9	6	5.5	15	15	15
sum	2.3	2.8	3.9	2.4	4.1	4.7	3.3	5	6.1	15	15	15

VRK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5652 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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VRK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5652 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments