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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

DGKH

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
DGKH N/A
Sequence Length (AA) Molecular Weight (Da)
1220 134866
Protein Name
Diacylglycerol kinase eta
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK
60 70 80 90 100
LIRKVSTSGQ IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS
110 120 130 140 150
KSLIFDEVDL SDASVAEAST KNANNSFTII TPFRRLMLCA ENRKEMEDWI
160 170 180 190 200
SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA CSHARPTFCN VCRESLSGVT
210 220 230 240 250
SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED EDGVAMPHQW
260 270 280 290 300
LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL
310 320 330 340 350
GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL
360 370 380 390 400
RRFKQLLNPA QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE
410 420 430 440 450
IDKLNLNKQC QLGVLPLGTG NDLARVLGWG GSYDDDTQLP QILEKLERAS
460 470 480 490 500
TKMLDRWSIM TYELKLPPKA SLLPGPPEAS EEFYMTIYED SVATHLTKIL
510 520 530 540 550
NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA DAVASKCSVL
560 570 580 590 600
NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG
610 620 630 640 650
DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA
660 670 680 690 700
NQSSDYDSTE TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV
710 720 730 740 750
PGPAVAASKE NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG
760 770 780 790 800
ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS
810 820 830 840 850
RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI
860 870 880 890 900
PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH
910 920 930 940 950
HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR
960 970 980 990 1000
AFESTLKSWE DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE
1010 1020 1030 1040 1050
ELITRICDAA TIHCLLEQEL AHAVNACSHA LNKANPRCPE SLTRDTATEI
1060 1070 1080 1090 1100
AINVKALYNE TESLLVGRVP LQLESPHEER VSNALHSVEV ELQKLTEIPW
1110 1120 1130 1140 1150
LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK QKTSSQPVQK
1160 1170 1180 1190 1200
WGTEEVAAWL DLLNLGEYKD IFIRHDIRGA ELLHLERRDL KDLGIPKVGH
1210 1220
VKRILQGIKE LGRSTPQSEV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5653 Collapse assay details

Data source: Panorama

Official Gene Symbol
DGKH
Peptide Sequence
GAELLHLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
1179
Peptide End
1187
CPTAC ID
non-CPTAC-5653
Peptide Molecular Mass
1,036.5665
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 1.5 1.6 6.1 1.9 2.1 5.5 2.4 2.6 8.2 15 15 15
y3 (1+) 2.4 2.7 11.4 4.5 2.6 15.3 5.1 3.7 19.1 15 15 15
y4 (1+) 1.5 1.7 4.4 2.3 2.6 5.5 2.7 3.1 7 15 15 15
sum 1.1 1.3 3.8 1.8 2 5.6 2.1 2.4 6.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments