STK32C, CPTAC-5654 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK32C	YANK3

Sequence Length (AA)	Molecular Weight (Da)
486	54994

Protein Name
Serine/threonine-protein kinase 32C

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRSGAERRGS	SAAASPGSPP	PGRARPAGSD	APSALPPPAA	GQPRARDSGD
60	70	80	90	100
VRSQPRPLFQ	WSKWKKRMGS	SMSAATARRP	VFDDKEDVNF	DHFQILRAIG
110	120	130	140	150
KGSFGKVCIV	QKRDTEKMYA	MKYMNKQQCI	ERDEVRNVFR	ELEILQEIEH
160	170	180	190	200
VFLVNLWYSF	QDEEDMFMVV	DLLLGGDLRY	HLQQNVQFSE	DTVRLYICEM
210	220	230	240	250
ALALDYLRGQ	HIIHRDVKPD	NILLDERGHA	HLTDFNIATI	IKDGERATAL
260	270	280	290	300
AGTKPYMAPE	IFHSFVNGGT	GYSFEVDWWS	VGVMAYELLR	GWRPYDIHSS
310	320	330	340	350
NAVESLVQLF	STVSVQYVPT	WSKEMVALLR	KLLTVNPEHR	LSSLQDVQAA
360	370	380	390	400
PALAGVLWDH	LSEKRVEPGF	VPNKGRLHCD	PTFELEEMIL	ESRPLHKKKK
410	420	430	440	450
RLAKNKSRDN	SRDSSQSEND	YLQDCLDAIQ	QDFVIFNREK	LKRSQDLPRE
460	470	480	486
PLPAPESRDA	AEPVEDEAER	SALPMCGPIC	PSAGSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5654 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK32C
Peptide Sequence: DAAEPVEDEAER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 459
Peptide End: 470

CPTAC ID: non-CPTAC-5654
Peptide Molecular Mass: 1,329.5685
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	0.7	0.6	1.9	1.4	2.1	1.6	1.6	2.2	2.5	15	15	15
y6 (1+)	1.1	0.8	1.7	1.5	1.2	2.5	1.9	1.4	3	15	15	15
y4 (1+)	1.3	1.2	3	2.5	1.3	3.3	2.8	1.8	4.5	15	15	15
sum	0.6	0.6	1.6	0.8	1.5	1.7	1	1.6	2.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5655 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK32C
Peptide Sequence: EPLPAPESR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 450
Peptide End: 458

CPTAC ID: non-CPTAC-5655
Peptide Molecular Mass: 994.5084
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend