NRK, CPTAC-5656 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NRK	N/A

Sequence Length (AA)	Molecular Weight (Da)
1582	178479

Protein Name
Nik-related protein kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGPGGWRDR	EVTDLGHLPD	PTGIFSLDKT	IGLGTYGRIY	LGLHEKTGAF
60	70	80	90	100
TAVKVMNARK	TPLPEIGRRV	RVNKYQKSVG	WRYSDEEEDL	RTELNLLRKY
110	120	130	140	150
SFHKNIVSFY	GAFFKLSPPG	QRHQLWMVME	LCAAGSVTDV	VRMTSNQSLK
160	170	180	190	200
EDWIAYICRE	ILQGLAHLHA	HRVIHRDIKG	QNVLLTHNAE	VKLVDFGVSA
210	220	230	240	250
QVSRTNGRRN	SFIGTPYWMA	PEVIDCDEDP	RRSYDYRSDV	WSVGITAIEM
260	270	280	290	300
AEGAPPLCNL	QPLEALFVIL	RESAPTVKSS	GWSRKFHNFM	EKCTIKNFLF
310	320	330	340	350
RPTSANMLQH	PFVRDIKNER	HVVESLTRHL	TGIIKKRQKK	GIPLIFEREE
360	370	380	390	400
AIKEQYTVRR	FRGPSCTHEL	LRLPTSSRCR	PLRVLHGEPS	QPRWLPDREE
410	420	430	440	450
PQVQALQQLQ	GAARVFMPLQ	ALDSAPKPLK	GQAQAPQRLQ	GAARVFMPLQ
460	470	480	490	500
AQVKAKASKP	LQMQIKAPPR	LRRAARVLMP	LQAQVRAPRL	LQVQSQVSKK
510	520	530	540	550
QQAQTQTSEP	QDLDQVPEEF	QGQDQVPEQQ	RQGQAPEQQQ	RHNQVPEQEL
560	570	580	590	600
EQNQAPEQPE	VQEQAAEPAQ	AETEAEEPES	LRVNAQVFLP	LLSQDHHVLL
610	620	630	640	650
PLHLDTQVLI	PVEGQTEGSP	QAQAWTLEPP	QAIGSVQALI	EGLSRDLLRA
660	670	680	690	700
PNSNNSKPLG	PLQTLMENLS	SNRFYSQPEQ	AREKKSKVST	LRQALAKRLS
710	720	730	740	750
PKRFRAKSSW	RPEKLELSDL	EARRQRRQRR	WEDIFNQHEE	ELRQVDKDKE
760	770	780	790	800
DESSDNDEVF	HSIQAEVQIE	PLKPYISNPK	KIEVQERSPS	VPNNQDHAHH
810	820	830	840	850
VKFSSSVPQR	SLLEQAQKPI	DIRQRSSQNR	QNWLAASESS	SEEESPVTGR
860	870	880	890	900
RSQSSPPYST	IDQKLLVDIH	VPDGFKVGKI	SPPVYLTNEW	VGYNALSEIF
910	920	930	940	950
RNDWLTPAPV	IQPPEEDGDY	VELYDASADT	DGDDDDESND	TFEDTYDHAN
960	970	980	990	1000
GNDDLDNQVD	QANDVCKDHD	DDNNKFVDDV	NNNYYEAPSC	PRASYGRDGS
1010	1020	1030	1040	1050
CKQDGYDGSR	GKEEAYRGYG	SHTANRSHGG	SAASEDNAAI	GDQEEHAANI
1060	1070	1080	1090	1100
GSERRGSEGD	GGKGVVRTSE	ESGALGLNGE	ENCSETDGPG	LKRPASQDFE
1110	1120	1130	1140	1150
YLQEEPGGGN	EASNAIDSGA	APSAPDHESD	NKDISESSTQ	SDFSANHSSP
1160	1170	1180	1190	1200
SKGSGMSADA	NFASAILYAG	FVEVPEESPK	QPSEVNVNPL	YVSPACKKPL
1210	1220	1230	1240	1250
IHMYEKEFTS	EICCGSLWGV	NLLLGTRSNL	YLMDRSGKAD	ITKLIRRRPF
1260	1270	1280	1290	1300
RQIQVLEPLN	LLITISGHKN	RLRVYHLTWL	RNKILNNDPE	SKRRQEEMLK
1310	1320	1330	1340	1350
TEEACKAIDK	LTGCEHFSVL	QHEETTYIAI	ALKSSIHLYA	WAPKSFDEST
1360	1370	1380	1390	1400
AIKVCIDQSA	DSEGDYMSYQ	AYIRILAKIQ	AADPVNRFKR	PDELLHLLKL
1410	1420	1430	1440	1450
KVFPTLDHKP	VTVDLAIGSE	KRLKIFFSSA	DGYHLIDAES	EVMSDVTLPK
1460	1470	1480	1490	1500
NPLEIIIPQN	IIILPDCLGI	GMMLTFNAEA	LSVEANEQLF	KKILEMWKDI
1510	1520	1530	1540	1550
PSSIAFECTQ	RTTGWGQKAI	EVRSLQSRVL	ESELKRRSIK	KLRFLCTRGD
1560	1570	1580	1582
KLFFTSTLRN	HHSRVYFMTL	GKLEELQSNY	DV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5656 Collapse assay details

Data source: Panorama

Official Gene Symbol: NRK
Peptide Sequence: EEPQVQALQQLQGAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 399
Peptide End: 414

CPTAC ID: non-CPTAC-5656
Peptide Molecular Mass: 1,764.9119
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	5.7	2.3	8.2	6.3	3.4	8.4	8.5	4.1	11.7	15	15	15
y11 (1+)	4.7	2.1	5.9	5.4	5	6.9	7.2	5.4	9.1	15	15	15
y10 (1+)	4.5	2.5	5.3	5.5	5.3	7.1	7.1	5.9	8.9	15	15	15
sum	4.7	2.1	2.9	5.5	4.5	4.3	7.2	5	5.2	15	15	15

NRK

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5656 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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NRK

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-5656 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments