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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SCYL2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SCYL2 CVAK104, KIAA1360
Sequence Length (AA) Molecular Weight (Da)
929 103709
Protein Name
SCY1-like protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESMLNKLKS TVTKVTADVT SAVMGNPVTR EFDVGRHIAS GGNGLAWKIF
60 70 80 90 100
NGTKKSTKQE VAVFVFDKKL IDKYQKFEKD QIIDSLKRGV QQLTRLRHPR
110 120 130 140 150
LLTVQHPLEE SRDCLAFCTE PVFASLANVL GNWENLPSPI SPDIKDYKLY
160 170 180 190 200
DVETKYGLLQ VSEGLSFLHS SVKMVHGNIT PENIILNKSG AWKIMGFDFC
210 220 230 240 250
VSSTNPSEQE PKFPCKEWDP NLPSLCLPNP EYLAPEYILS VSCETASDMY
260 270 280 290 300
SLGTVMYAVF NKGKPIFEVN KQDIYKSFSR QLDQLSRLGS SSLTNIPEEV
310 320 330 340 350
REHVKLLLNV TPTVRPDADQ MTKIPFFDDV GAVTLQYFDT LFQRDNLQKS
360 370 380 390 400
QFFKGLPKVL PKLPKRVIVQ RILPCLTSEF VNPDMVPFVL PNVLLIAEEC
410 420 430 440 450
TKEEYVKLIL PELGPVFKQQ EPIQILLIFL QKMDLLLTKT PPDEIKNSVL
460 470 480 490 500
PMVYRALEAP SIQIQELCLN IIPTFANLID YPSMKNALIP RIKNACLQTS
510 520 530 540 550
SLAVRVNSLV CLGKILEYLD KWFVLDDILP FLQQIPSKEP AVLMGILGIY
560 570 580 590 600
KCTFTHKKLG ITKEQLAGKV LPHLIPLSIE NNLNLNQFNS FISVIKEMLN
610 620 630 640 650
RLESEHKTKL EQLHIMQEQQ KSLDIGNQMN VSEEMKVTNI GNQQIDKVFN
660 670 680 690 700
NIGADLLTGS ESENKEDGLQ NKHKRASLTL EEKQKLAKEQ EQAQKLKSQQ
710 720 730 740 750
PLKPQVHTPV ATVKQTKDLT DTLMDNMSSL TSLSVSTPKS SASSTFTSVP
760 770 780 790 800
SMGIGMMFST PTDNTKRNLT NGLNANMGFQ TSGFNMPVNT NQNFYSSPST
810 820 830 840 850
VGVTKMTLGT PPTLPNFNAL SVPPAGAKQT QQRPTDMSAL NNLFGPQKPK
860 870 880 890 900
VSMNQLSQQK PNQWLNQFVP PQGSPTMGSS VMGTQMNVIG QSAFGMQGNP
910 920 929
FFNPQNFAQP PTTMTNSSSA SNDLKDLFG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5657 Collapse assay details

Data source: Panorama

Official Gene Symbol
SCYL2
Peptide Sequence
LGSSSLTNIPEEVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
288
Peptide End
301
CPTAC ID
non-CPTAC-5657
Peptide Molecular Mass
1,500.7784
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 3.7 3.5 2.7 4.4 4.6 3.2 5.7 5.8 4.2 15 15 15
y7 (1+) 5 4.5 4.1 4.5 4.3 4.6 6.7 6.2 6.2 15 15 15
sum 4 3.5 1.7 4.6 4.4 2.7 6.1 5.6 3.2 15 15 15
y5 (1+) 4 3.4 1.9 5.3 4.7 2.8 6.6 5.8 3.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments