MAST2, CPTAC-5658 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAST2	KIAA0807, MAST205

Sequence Length (AA)	Molecular Weight (Da)
1798	196436

Protein Name
Microtubule-associated serine/threonine-protein kinase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKRSRCRDRP	QPPPPDRRED	GVQRAAELSQ	SLPPRRRAPP	GRQRLEERTG
60	70	80	90	100
PAGPEGKEQD	VVTGVSPLLF	RKLSNPDIFS	STGKVKLQRQ	LSQDDCKLWR
110	120	130	140	150
GNLASSLSGK	QLLPLSSSVH	SSVGQVTWQS	SGEASNLVRM	RNQSLGQSAP
160	170	180	190	200
SLTAGLKELS	LPRRGSFCRT	SNRKSLIVTS	STSPTLPRPH	SPLHGHTGNS
210	220	230	240	250
PLDSPRNFSP	NAPAHFSFVP	ARRTDGRRWS	LASLPSSGYG	TNTPSSTVSS
260	270	280	290	300
SCSSQEKLHQ	LPFQPTADEL	HFLTKHFSTE	SVPDEEGRQS	PAMRPRSRSL
310	320	330	340	350
SPGRSPVSFD	SEIIMMNHVY	KERFPKATAQ	MEERLAEFIS	SNTPDSVLPL
360	370	380	390	400
ADGALSFIHH	QVIEMARDCL	DKSRSGLITS	QYFYELQDNL	EKLLQDAHER
410	420	430	440	450
SESSEVAFVM	QLVKKLMIII	ARPARLLECL	EFDPEEFYHL	LEAAEGHAKE
460	470	480	490	500
GQGIKCDIPR	YIVSQLGLTR	DPLEEMAQLS	SCDSPDTPET	DDSIEGHGAS
510	520	530	540	550
LPSKKTPSEE	DFETIKLISN	GAYGAVFLVR	HKSTRQRFAM	KKINKQNLIL
560	570	580	590	600
RNQIQQAFVE	RDILTFAENP	FVVSMFCSFD	TKRHLCMVME	YVEGGDCATL
610	620	630	640	650
LKNIGALPVD	MVRLYFAETV	LALEYLHNYG	IVHRDLKPDN	LLITSMGHIK
660	670	680	690	700
LTDFGLSKIG	LMSLTTNLYE	GHIEKDAREF	LDKQVCGTPE	YIAPEVILRQ
710	720	730	740	750
GYGKPVDWWA	MGIILYEFLV	GCVPFFGDTP	EELFGQVISD	EIVWPEGDEA
760	770	780	790	800
LPPDAQDLTS	KLLHQNPLER	LGTGSAYEVK	QHPFFTGLDW	TGLLRQKAEF
810	820	830	840	850
IPQLESEDDT	SYFDTRSERY	HHMDSEDEEE	VSEDGCLEIR	QFSSCSPRFN
860	870	880	890	900
KVYSSMERLS	LLEERRTPPP	TKRSLSEEKE	DHSDGLAGLK	GRDRSWVIGS
910	920	930	940	950
PEILRKRLSV	SESSHTESDS	SPPMTVRRRC	SGLLDAPRFP	EGPEEASSTL
960	970	980	990	1000
RRQPQEGIWV	LTPPSGEGVS	GPVTEHSGEQ	RPKLDEEAVG	RSSGSSPAME
1010	1020	1030	1040	1050
TRGRGTSQLA	EGATAKAISD	LAVRRARHRL	LSGDSTEKRT	ARPVNKVIKS
1060	1070	1080	1090	1100
ASATALSLLI	PSEHHTCSPL	ASPMSPHSQS	SNPSSRDSSP	SRDFLPALGS
1110	1120	1130	1140	1150
MRPPIIIHRA	GKKYGFTLRA	IRVYMGDSDV	YTVHHMVWHV	EDGGPASEAG
1160	1170	1180	1190	1200
LRQGDLITHV	NGEPVHGLVH	TEVVELILKS	GNKVAISTTP	LENTSIKVGP
1210	1220	1230	1240	1250
ARKGSYKAKM	ARRSKRSRGK	DGQESRKRSS	LFRKITKQAS	LLHTSRSLSS
1260	1270	1280	1290	1300
LNRSLSSGES	GPGSPTHSHS	LSPRSPTQGY	RVTPDAVHSV	GGNSSQSSSP
1310	1320	1330	1340	1350
SSSVPSSPAG	SGHTRPSSLH	GLAPKLQRQY	RSPRRKSAGS	IPLSPLAHTP
1360	1370	1380	1390	1400
SPPPPTASPQ	RSPSPLSGHV	AQAFPTKLHL	SPPLGRQLSR	PKSAEPPRSP
1410	1420	1430	1440	1450
LLKRVQSAEK	LAAALAASEK	KLATSRKHSL	DLPHSELKKE	LPPREVSPLE
1460	1470	1480	1490	1500
VVGARSVLSG	KGALPGKGVL	QPAPSRALGT	LRQDRAERRE	SLQKQEAIRE
1510	1520	1530	1540	1550
VDSSEDDTEE	GPENSQGAQE	LSLAPHPEVS	QSVAPKGAGE	SGEEDPFPSR
1560	1570	1580	1590	1600
DPRSLGPMVP	SLLTGITLGP	PRMESPSGPH	RRLGSPQAIE	EAASSSSAGP
1610	1620	1630	1640	1650
NLGQSGATDP	IPPEGCWKAQ	HLHTQALTAL	SPSTSGLTPT	SSCSPPSSTS
1660	1670	1680	1690	1700
GKLSMWSWKS	LIEGPDRASP	SRKATMAGGL	ANLQDLENTT	PAQPKNLSPR
1710	1720	1730	1740	1750
EQGKTQPPSA	PRLAHPSYED	PSQGWLWESE	CAQAVKEDPA	LSITQVPDAS
1760	1770	1780	1790	1798
GDRRQDVPCR	GCPLTQKSEP	SLRRGQEPGG	HQKHRDLALV	PDELLKQT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-5658 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAST2
Peptide Sequence: EDPALSITQVPDASGDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1737
Peptide End: 1753

CPTAC ID: non-CPTAC-5658
Peptide Molecular Mass: 1,769.8432
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	0.9	2.1	5.2	2.2	2.1	5.4	2.4	3	7.5	15	15	15
y10 (1+)	1.5	1.4	4.1	2.4	2	6.6	2.8	2.4	7.8	15	15	15
y12 (1+)	1.8	1.4	3.9	3	1.7	5.1	3.5	2.2	6.4	15	15	15
sum	0.9	1	3.3	2.3	1.5	3.8	2.5	1.8	5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-5659 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAST2
Peptide Sequence: VAISTTPLENTSIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 1184
Peptide End: 1197

CPTAC ID: non-CPTAC-5659
Peptide Molecular Mass: 1,472.8086
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend